MDC antibody
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Rabbit anti Human MDC antibody recognizes human MDC, also known as macrophage-derived cytokine, CCL22, CC chemokine STCP-1, Small-inducible cytokine A22 or Stimulated T-cell chemotactic protein 1. MDC is a 93 amino acid, ~10.6 kDa member of the CC chemokine family expressed by macrophages, monocyte-derived dendritic cells, epithelial cells, fibroblasts and highly expressed in the thymus.
MDC acts as a chemoattractant for monocytes, dendritic cells, activated T lymphocytes and NK cells, through binding to chemokine (C-C motif) receptor 4 (CCR4). CCR4 is selectively expressed by Th2 cells and several studies have revealed a link between MDC and another ligand of CCR4, known as activation-regulated chemokine (TARC/CCL17), with Th2-mediated inflammatory disorders such as atopic dermatitis and ulcerative colitis (Judge et al. 2001).
MDC acts as a chemoattractant for monocytes, dendritic cells, activated T lymphocytes and NK cells, through binding to chemokine (C-C motif) receptor 4 (CCR4). CCR4 is selectively expressed by Th2 cells and several studies have revealed a link between MDC and another ligand of CCR4, known as activation-regulated chemokine (TARC/CCL17), with Th2-mediated inflammatory disorders such as atopic dermatitis and ulcerative colitis (Judge et al. 2001).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
- Antiserum Preparation
- Antisera to human MDC were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human MDC.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O00626
- Entrez Gene
- CCL22
- GO Terms
- GO:0009615 response to virus
- GO:0007165 signal transduction
- GO:0005615 extracellular space
- GO:0006935 chemotaxis
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0007267 cell-cell signaling
- GO:0008009 chemokine activity
- Regulatory
- For research purposes only
Applications of MDC antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.5ug/ml | ||
| Functional Assays | 5.0 | 7.0ug/ml | |
| Western Blotting | 0.1 | 0.2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Imai, T. et al. (1998) Macrophage-derived chemokine is a functional ligand for the CC chemokine receptor 4.
J Biol Chem. 273 (3): 1764-8.
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