MAP1LC3A/B antibody

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Anti Human MAP1LC3A/B (N-Terminal) Antibody thumbnail image 1

Anti Human MAP1LC3A/B (N-Terminal) Antibody thumbnail image 2

Anti Human MAP1LC3A/B (N-Terminal) Antibody thumbnail image 3

Anti Human MAP1LC3A/B (N-Terminal) Antibody thumbnail image 4

Anti Human MAP1LC3A/B (N-Terminal) Antibody gallery image 1 — HeLa lysate probed with Rabbit anti Human MAP1LC3A/B (N-terminal) (**AHP2167**) followed by horseradish peroxidase conjugated Sheep anti Rabbit IgG antibody.

Anti Human MAP1LC3A/B (N-Terminal) Antibody gallery image 2 — Western blotting analysis of LC3 expression on Atg5 knockout and wild type cells using Rabbit anti Human MAP1LC3A/B (**AHP2167**). Image courtesy of Dr. R. Kapito, Stanford University

Anti Human MAP1LC3A/B (N-Terminal) Antibody gallery image 3 — **Published customer image:**
Rabbit anti Human MAP1LC3A/B antibody used for the detection of LC3-I and LC3-II in human mammary epithelial whole cell lysates by western blotting.
**Image caption:**
D, Western blotting for LC3-I and LC3-II from HMEC-LXSN, HMEC-WT-1/WT-2, and HMEC-cyto lysates treated with ethanol control, 1 μM Tam, or 2 μM Tam for 48 hours. E, Western blot for LC3-II of whole cell lysates from HMECs expressing control or ERRγ overexpression vector, treated with control or 0.5, 1, or 2 μM Tam for 24 hours. F, Western blot for LC3 from whole cell lysates of HMEC-LXSN and HMEC-cyto cells expressing either shControl or shERRγ, treated with ethanol control or 1 μM Tam for 24 hours.

From: Girard BJ, Regan Anderson TM, Welch SL, Nicely J, Seewaldt VL, *et al*. (2015)
Cytoplasmic PELP1 and ERRgamma Protect Human Mammary Epithelial Cells from Tam-Induced Cell Death.
PLoS ONE 10(3): e0121206.

Anti Human MAP1LC3A/B (N-Terminal) Antibody gallery image 4 — HeLA cells were fixed with 3% PFA and permeabilized with 0.3% Triton X-100. Nuclei were stained with 1.5 µg/ml DAPI. Immunofluorescence was carried out using **AHP2167**, a Rabbit polyclonal anti-Human Microtubule associated protein 1LC3A/B (MAP1LC3A/B) at 1/25. Sheep anti-Rabbit DyLight^®488 (STAR36D488GA) was then applied at 1/50. Image was visualized on the Bio-Rad ZOE^™ Fluorescent Cell Imager at 60x magnification.

Rabbit anti Human MAP1LC3A/B (N-Terminal)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP2167T	IF WB			25 µg
AHP2167	IF WB			0.1 mg

Rabbit anti Human MAP1LC3A/B (N-Terminal) antibody specifically recognizes an epitope within the N-Terminal (NT) region of both MAP1LC3A (Microtubule-associated proteins 1A/1B light chain 3A/LC3A) and MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B/LC3B), ubiquitin-like proteins and members of the MAP1LC3 family, which are widely used as reliable markers for the monitoring of autophagy.

LC3-I is the cytosolic form of LC3, which is converted into the active, membrane-bound form LC3-II, during the autophagy process. Tracking the level of conversion of LC3-I to LC3-II provides an indicator of autophagic activity, and levels of LC3-II in particular, correlate with the extent of autophagosome formation, due to its association with the autophagosome membrane.

Rabbit anti Human MAP1LC3A/B (N-Terminal) antibody recognizes both the LC3-I and LC3-II forms of MAP1LC3A and MAP1LC3B.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antiserum to human LC3A was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

Synthetic peptide sequence PSDRPFKQRRSFADC from the N-Terminal region of LC3A (NP_115903.1; NP_852610.1).

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: Q9H492 Related reagents; Q9GZQ8 Related reagents; Q91VR7 Related reagents; Q9CQV6 Related reagents
Entrez Gene: Map1lc3a Related reagents; Map1lc3b Related reagents; MAP1LC3B Related reagents; MAP1LC3A Related reagents
GO Terms: GO:0000045 autophagic vacuole assembly; GO:0005515 protein binding; GO:0000421 autophagic vacuole membrane; GO:0005874 microtubule; GO:0012505 endomembrane system; GO:0031410 cytoplasmic vesicle; GO:0006914 autophagy; GO:0005829 cytosol; GO:0008429 phosphatidylethanolamine binding
Regulatory: For research purposes only

Applications of MAP1LC3A/B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunofluorescence
Western Blotting		1/100	1/250

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP2167 detects a band of approximately 14-15kDa corresponding to LC3-II, and a band of approximately 17kDa corresponding to LC3-I, in HeLa cell lysates.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Sheep anti Rabbit IgG:Biotin	2AB02B	1 ml	C E P WB
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	1 mg	E WB
Goat anti Rabbit IgG (Fc):FITC	STAR121F	1 mg	F
Goat anti Rabbit IgG (Fc):HRP	STAR121P	1 mg	E WB
Goat anti Rabbit IgG (H/L):HRP	STAR124P	1 mg	C E WB
Sheep anti Rabbit IgG:FITC	STAR34B	1 mg	C F
Sheep anti Rabbit IgG:RPE	STAR35A	1 ml	F
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:DyLight®549	STAR36D549GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®649	STAR36D649GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	0.1 mg	F IF WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	0.5 ml	WB*

Application Based External Images

Immunofluorescence

Western Blotting

Product Specific References

References for MAP1LC3A/B antibody

Iwata, A. et al. (2005) HDAC6 and microtubules are required for autophagic degradation of aggregated huntingtin.
J Biol Chem. 280 (48): 40282-92.
Riley, B.E. et al. (2010) Ubiquitin accumulation in autophagy-deficient mice is dependent on the Nrf2-mediated stress response pathway: a potential role for protein aggregation in autophagic substrate selection.
J Cell Biol. 191 (3): 537-52.
Gjyshi, O. et al. (2015) Kaposi's Sarcoma-Associated Herpesvirus Induces Nrf2 Activation in Latently Infected Endothelial Cells through SQSTM1 Phosphorylation and Interaction with Polyubiquitinated Keap1.
J Virol. 89: 2268-86
Huang, L. et al. (2014) AKI after conditional and kidney-specific knockdown of stanniocalcin-1.
J Am Soc Nephrol. 25: 2303-15.
Girard, B.J. et al. (2015) Cytoplasmic PELP1 and ERRgamma Protect Human Mammary Epithelial Cells from Tam-Induced Cell Death.
PLoS One. 10 (3): e0121206.

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