MAP1LC3A/B antibody
Rabbit anti Human MAP1LC3A/B antibody used for the detection of LC3-I and LC3-II in human mammary epithelial whole cell lysates by western blotting.
Image caption:
D, Western blotting for LC3-I and LC3-II from HMEC-LXSN, HMEC-WT-1/WT-2, and HMEC-cyto lysates treated with ethanol control, 1 μM Tam, or 2 μM Tam for 48 hours. E, Western blot for LC3-II of whole cell lysates from HMECs expressing control or ERRγ overexpression vector, treated with control or 0.5, 1, or 2 μM Tam for 24 hours. F, Western blot for LC3 from whole cell lysates of HMEC-LXSN and HMEC-cyto cells expressing either shControl or shERRγ, treated with ethanol control or 1 μM Tam for 24 hours.
From: Girard BJ, Regan Anderson TM, Welch SL, Nicely J, Seewaldt VL, et al. (2015)
Cytoplasmic PELP1 and ERRgamma Protect Human Mammary Epithelial Cells from Tam-Induced Cell Death.
PLoS ONE 10(3): e0121206.
Rabbit anti Human MAP1LC3A/B (N-Terminal) antibody specifically recognizes an epitope within the N-Terminal (NT) region of both MAP1LC3A (Microtubule-associated proteins 1A/1B light chain 3A/LC3A) and MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B/LC3B), ubiquitin-like proteins and members of the MAP1LC3 family, which are widely used as reliable markers for the monitoring of autophagy.
LC3-I is the cytosolic form of LC3, which is converted into the active, membrane-bound form LC3-II, during the autophagy process. Tracking the level of conversion of LC3-I to LC3-II provides an indicator of autophagic activity, and levels of LC3-II in particular, correlate with the extent of autophagosome formation, due to its association with the autophagosome membrane.
Rabbit anti Human MAP1LC3A/B (N-Terminal) antibody recognizes both the LC3-I and LC3-II forms of MAP1LC3A and MAP1LC3B.
LC3-I is the cytosolic form of LC3, which is converted into the active, membrane-bound form LC3-II, during the autophagy process. Tracking the level of conversion of LC3-I to LC3-II provides an indicator of autophagic activity, and levels of LC3-II in particular, correlate with the extent of autophagosome formation, due to its association with the autophagosome membrane.
Rabbit anti Human MAP1LC3A/B (N-Terminal) antibody recognizes both the LC3-I and LC3-II forms of MAP1LC3A and MAP1LC3B.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human LC3A was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide sequence PSDRPFKQRRSFADC from the N-Terminal region of LC3A (NP_115903.1; NP_852610.1).
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9H492
- Q9GZQ8
- Q91VR7
- Q9CQV6
- Entrez Gene
- Map1lc3a
- Map1lc3b
- MAP1LC3B
- MAP1LC3A
- GO Terms
- GO:0000045 autophagic vacuole assembly
- GO:0005515 protein binding
- GO:0000421 autophagic vacuole membrane
- GO:0005874 microtubule
- GO:0012505 endomembrane system
- GO:0031410 cytoplasmic vesicle
- GO:0006914 autophagy
- GO:0005829 cytosol
- GO:0008429 phosphatidylethanolamine binding
- Regulatory
- For research purposes only
Applications of MAP1LC3A/B antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | |||
| Western Blotting | 1/1000 | 1/5000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP2167 detects a band of approximately 14-15kDa corresponding to LC3-II, and a band of approximately 17kDa corresponding to LC3-I, in HeLa cell lysates.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for MAP1LC3A/B antibody
-
Iwata, A. et al. (2005) HDAC6 and microtubules are required for autophagic degradation of aggregated huntingtin.
J Biol Chem. 280 (48): 40282-92. -
Riley, B.E. et al. (2010) Ubiquitin accumulation in autophagy-deficient mice is dependent on the Nrf2-mediated stress response pathway: a potential role for protein aggregation in autophagic substrate selection.
J Cell Biol. 191 (3): 537-52. -
Gjyshi, O. et al. (2015) Kaposi's Sarcoma-Associated Herpesvirus Induces Nrf2 Activation in Latently Infected Endothelial Cells through SQSTM1 Phosphorylation and Interaction with Polyubiquitinated Keap1.
J Virol. 89: 2268-86 -
Huang, L. et al. (2014) AKI after conditional and kidney-specific knockdown of stanniocalcin-1.
J Am Soc Nephrol. 25: 2303-15. -
Girard, B.J. et al. (2015) Cytoplasmic PELP1 and ERRgamma Protect Human Mammary Epithelial Cells from Tam-Induced Cell Death.
PLoS One. 10 (3): e0121206.
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