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MAP1LC3A/B antibody

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Anti Human MAP1LC3A/B (N-Terminal) Antibody thumbnail image 1 Anti Human MAP1LC3A/B (N-Terminal) Antibody thumbnail image 2 Anti Human MAP1LC3A/B (N-Terminal) Antibody thumbnail image 3 Anti Human MAP1LC3A/B (N-Terminal) Antibody thumbnail image 4
Anti Human MAP1LC3A/B (N-Terminal) Antibody gallery image 1
HeLa lysate probed with Rabbit anti Human MAP1LC3A/B (N-terminal) (AHP2167) followed by horseradish peroxidase conjugated Sheep anti Rabbit IgG antibody.
Anti Human MAP1LC3A/B (N-Terminal) Antibody gallery image 2
Western blotting analysis of LC3 expression on Atg5 knockout and wild type cells using Rabbit anti Human MAP1LC3A/B (AHP2167). Image courtesy of Dr. R. Kapito, Stanford University
Anti Human MAP1LC3A/B (N-Terminal) Antibody gallery image 3
Published customer image:
Rabbit anti Human MAP1LC3A/B antibody used for the detection of LC3-I and LC3-II in human mammary epithelial whole cell lysates by western blotting.
Image caption:
D, Western blotting for LC3-I and LC3-II from HMEC-LXSN, HMEC-WT-1/WT-2, and HMEC-cyto lysates treated with ethanol control, 1 μM Tam, or 2 μM Tam for 48 hours. E, Western blot for LC3-II of whole cell lysates from HMECs expressing control or ERRγ overexpression vector, treated with control or 0.5, 1, or 2 μM Tam for 24 hours. F, Western blot for LC3 from whole cell lysates of HMEC-LXSN and HMEC-cyto cells expressing either shControl or shERRγ, treated with ethanol control or 1 μM Tam for 24 hours.

From: Girard BJ, Regan Anderson TM, Welch SL, Nicely J, Seewaldt VL, et al. (2015)
Cytoplasmic PELP1 and ERRgamma Protect Human Mammary Epithelial Cells from Tam-Induced Cell Death.
PLoS ONE 10(3): e0121206.
Anti Human MAP1LC3A/B (N-Terminal) Antibody gallery image 4
HeLA cells were fixed with 3% PFA and permeabilized with 0.3% Triton X-100. Nuclei were stained with 1.5 µg/ml DAPI. Immunofluorescence was carried out using AHP2167, a Rabbit polyclonal anti-Human Microtubule associated protein 1LC3A/B (MAP1LC3A/B) at 1/25. Sheep anti-Rabbit DyLight®488 (STAR36D488GA) was then applied at 1/50. Image was visualized on the Bio-Rad ZOE™ Fluorescent Cell Imager at 60x magnification.

Rabbit anti Human MAP1LC3A/B (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2167T
Datasheet
IF WB
MSDS
25 µg loader

AHP2167
Datasheet
IF WB
MSDS
0.1 mg loader

Rabbit anti Human MAP1LC3A/B (N-Terminal) antibody specifically recognizes an epitope within the N-Terminal (NT) region of both MAP1LC3A (Microtubule-associated proteins 1A/1B light chain 3A/LC3A) and MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B/LC3B), ubiquitin-like proteins and members of the MAP1LC3 family, which are widely used as reliable markers for the monitoring of autophagy.

LC3-I is the cytosolic form of LC3, which is converted into the active, membrane-bound form LC3-II, during the autophagy process. Tracking the level of conversion of LC3-I to LC3-II provides an indicator of autophagic activity, and levels of LC3-II in particular, correlate with the extent of autophagosome formation, due to its association with the autophagosome membrane.

Rabbit anti Human MAP1LC3A/B (N-Terminal) antibody recognizes both the LC3-I and LC3-II forms of MAP1LC3A and MAP1LC3B.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human LC3A was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide sequence PSDRPFKQRRSFADC from the N-Terminal region of LC3A (NP_115903.1; NP_852610.1).
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
Q9H492
Q9GZQ8
Q91VR7
Q9CQV6
Entrez Gene
Map1lc3a
Map1lc3b
MAP1LC3B
MAP1LC3A
GO Terms
GO:0000045 autophagic vacuole assembly
GO:0005515 protein binding
GO:0000421 autophagic vacuole membrane
GO:0005874 microtubule
GO:0012505 endomembrane system
GO:0031410 cytoplasmic vesicle
GO:0006914 autophagy
GO:0005829 cytosol
GO:0008429 phosphatidylethanolamine binding
Regulatory
For research purposes only

Applications of MAP1LC3A/B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Western Blotting 1/100 1/250
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2167 detects a band of approximately 14-15kDa corresponding to LC3-II, and a band of approximately 17kDa corresponding to LC3-I, in HeLa cell lysates.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Application Based External Images

Immunofluorescence

Western Blotting

Product Specific References

References for MAP1LC3A/B antibody

  1. Iwata, A. et al. (2005) HDAC6 and microtubules are required for autophagic degradation of aggregated huntingtin.
    J Biol Chem. 280 (48): 40282-92.
  2. Riley, B.E. et al. (2010) Ubiquitin accumulation in autophagy-deficient mice is dependent on the Nrf2-mediated stress response pathway: a potential role for protein aggregation in autophagic substrate selection.
    J Cell Biol. 191 (3): 537-52.
  3. Gjyshi, O. et al. (2015) Kaposi's Sarcoma-Associated Herpesvirus Induces Nrf2 Activation in Latently Infected Endothelial Cells through SQSTM1 Phosphorylation and Interaction with Polyubiquitinated Keap1.
    J Virol. 89: 2268-86
  4. Huang, L. et al. (2014) AKI after conditional and kidney-specific knockdown of stanniocalcin-1.
    J Am Soc Nephrol. 25: 2303-15.
  5. Girard, B.J. et al. (2015) Cytoplasmic PELP1 and ERRgamma Protect Human Mammary Epithelial Cells from Tam-Induced Cell Death.
    PLoS One. 10 (3): e0121206.
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