Leptin antibody

Rabbit anti Human Leptin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Leptin

Product Code Applications Pack Size List Price Your Price Qty
AHP1616
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E P * WB 0.1 mg loader
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Rabbit anti Human Leptin antibody recognizes human leptin, a ~16 kDa major adipose-derived hormone and member of the leptin family, which plays a key role in appetite control and the regulation of energy expenditure.

Secreted leptin associates with proteins within the bloodstream and is transported to the brain, where it inhibits or up-regulates the activity of neuropeptides such as neuropeptide Y (NPY) and alpha-melanocyte-stimulating hormone respectively. Leptin is a recognised marker of obesity, with a correlation between circulating levels of leptin and body fat mass, and defects in leptin production result in severe obesity. Regulators of leptin expression include insulin, cortisol, cAMP and thiazolidinediones.

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antiserum to human leptin was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant human leptin (PHP198)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
6 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Immunohistology - Paraffin 1 0.5ug/ml
Western Blotting 0.1 0.2ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This purified human leptin antibody may be used in an ELISA with recombinant human leptin (PHP198) as the standard.
Western Blotting
This purified human leptin antibody may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human leptin (PHP198) as the positive control.

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References for Leptin antibody

  1. Erdoğan, M. et al. (2022) T he role of leptin in primary Sj&0uml;gren syndrome: a clinical and histopathological assessment study.
    Turkish J Med Sci. 52: 524-7.

Further Reading

  1. Paracchini, V. et al. (2005) Genetics of leptin and obesity: a HuGE review.
    Am J Epidemiol. 162 (2): 101-14.

Synonyms
Obesity Factor
RRID
AB_2135946
UniProt
P41159
Entrez Gene
LEP
GO Terms
GO:0005615 extracellular space
GO:0006112 energy reserve metabolic process
GO:0008343 adult feeding behavior
GO:0032099 negative regulation of appetite
View more products with LEPTIN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products