Leptin antibody



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P ResetRabbit anti Human Leptin
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Leptin
Rabbit anti Human Leptin antibody recognizes human leptin, a ~16 kDa major adipose-derived hormone and member of the leptin family, which plays a key role in appetite control and the regulation of energy expenditure. Secreted leptin associates with proteins within the bloodstream and is transported to the brain, where it inhibits or up-regulates the activity of neuropeptides such as neuropeptide Y (NPY) and alpha-melanocyte-stimulating hormone respectively. Leptin is a recognised marker of obesity, with a correlation between circulating levels of leptin and body fat mass, and defects in leptin production result in severe obesity. Regulators of leptin expression include insulin, cortisol, cAMP and thiazolidinediones. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antiserum to human leptin was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human leptin (PHP198)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | ![]() |
0.5 | 2.0ug/ml |
Immunohistology - Paraffin 1 | ![]() |
0.5ug/ml | |
Western Blotting | ![]() |
0.1 | 0.2ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
- ELISA
- This purified human leptin antibody may be used in an ELISA with recombinant human leptin (PHP198) as the standard.
- Western Blotting
- This purified human leptin antibody may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human leptin (PHP198) as the positive control.
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Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
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Description | Antigen Retrieval Buffer, pH8.0 | ||||||
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | Log in | |||
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Description | TidyBlot Western Blot Detection Reagent:HRP |
References for Leptin antibody
-
Erdoğan, M. et al. (2022) T he role of leptin in primary Sj&0uml;gren syndrome: a clinical and histopathological assessment study.
Turkish J Med Sci. 52: 524-7.
Further Reading
-
Paracchini, V. et al. (2005) Genetics of leptin and obesity: a HuGE review.
Am J Epidemiol. 162 (2): 101-14.
- Synonyms
- Obesity Factor
- RRID
- AB_2135946
- UniProt
- P41159
- Entrez Gene
- LEP
- GO Terms
- GO:0005615 extracellular space
- GO:0006112 energy reserve metabolic process
- GO:0008343 adult feeding behavior
- GO:0032099 negative regulation of appetite
AHP1616



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