Leptin antibody
Rabbit anti Human Leptin antibody (AHP1616) used to label leptin in Human salviry glands by immunohistochemistry.
Image caption:
Examples for leptin staining patterns in minor salivary glands. a) Diffuse leptin staining (score 3) in acinar, ductal, and stromal areas in a minor salivary gland (× 400 magnification). b) Moderate (score 2) leptin staining in acinar and ductal areas in and diffuse leptin staining (score 3) in the stromal area in minor salivary gland (× 400 magnification). c) Diffuse leptin staining (score 3) in acinar and ductal minor areas and mild (score1) leptin staining in the stromal area in a salivary gland. d) No leptin staining in acinar and ductal areas and mild leptin staining (score 1) in the stromal area in minor salivary gland (× 200 magnification).
From: Erdoğan, M. et al.
The role of leptin in primary Sjögren syndrome: a clinical and histopathological assessment study
Turkish J Med Sci: 52: 524-7.
doi: 10.3906/sag-2108-295
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
P ResetRabbit anti Human Leptin
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Leptin
Antibody Quality and Performance Guarantee
| Rabbit anti Human Leptin antibody recognizes human leptin, a ~16 kDa major adipose-derived hormone and member of the leptin family, which plays a key role in appetite control and the regulation of energy expenditure. Secreted leptin associates with proteins within the bloodstream and is transported to the brain, where it inhibits or up-regulates the activity of neuropeptides such as neuropeptide Y (NPY) and alpha-melanocyte-stimulating hormone respectively. Leptin is a recognised marker of obesity, with a correlation between circulating levels of leptin and body fat mass, and defects in leptin production result in severe obesity. Regulators of leptin expression include insulin, cortisol, cAMP and thiazolidinediones. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antiserum to human leptin was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human leptin (PHP198)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0ug/ml |
| Immunohistology - Paraffin 1 | |
0.5ug/ml | |
| Western Blotting | |
0.1 | 0.2ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This purified human leptin antibody may be used in an ELISA with recombinant human leptin (PHP198) as the standard.
- Western Blotting
- This purified human leptin antibody may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human leptin (PHP198) as the positive control.
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| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | Log in | |||
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References for Leptin antibody
-
Erdoğan, M. et al. (2022) T he role of leptin in primary Sj&0uml;gren syndrome: a clinical and histopathological assessment study.
Turkish J Med Sci. 52: 524-7.
Further Reading
-
Paracchini, V. et al. (2005) Genetics of leptin and obesity: a HuGE review.
Am J Epidemiol. 162 (2): 101-14.
- Synonyms
- Obesity Factor
- RRID
- AB_2135946
- UniProt
- P41159
- Entrez Gene
- LEP
- GO Terms
- GO:0005615 extracellular space
- GO:0006112 energy reserve metabolic process
- GO:0008343 adult feeding behavior
- GO:0032099 negative regulation of appetite
AHP1616
149046
153848
154744
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