Kappa Light Chain antibody

Goat F(ab')2 anti Human Kappa Light Chain:FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Kappa Light Chain

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Goat F(ab')2 anti Human Kappa Light Chain antibody recognizes human kappa chains, and demonstrates <1% cross-reactivity with other human immunoglobulin classes or subclasses.

Target Species
Human
Product Form
F(ab')2 fragments of purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Antiserum Preparation
Antisera to human kappa chains were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Human kappa chains.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. Prior to reconstitution store at +4°C.
After reconstitution it is recommended to aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/200
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul.

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RRID
AB_999298
UniProt
P01834
Entrez Gene
IGKC
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
View more products with KAPPA LIGHT CHAIN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products