Kappa Light Chain antibody

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Goat F(ab')2 anti Human Kappa Light Chain:FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
STAR101FB C F 1 mg
Goat F(ab')2 anti Human Kappa Light Chain antibody recognizes human kappa chains, and demonstrates <1% cross-reactivity with other human immunoglobulin classes or subclasses.

Product Details

Target Species
Human
Product Form
F(ab')2 fragments of purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Antiserum Preparation
Antisera to human kappa chains were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Human kappa chains.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01834 Related reagents
Entrez Gene
IGKC Related reagents
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
Regulatory
For research purposes only

Applications of Kappa Light Chain antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/200
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F