Kappa Light Chain antibody
Goat F(ab')2 anti Human Kappa Light Chain:FITC
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Kappa Light Chain
Goat F(ab')2 anti Human Kappa Light Chain antibody recognizes human kappa chains, and demonstrates <1% cross-reactivity with other human immunoglobulin classes or subclasses. |
- Target Species
- Human
- Product Form
- F(ab')2 fragments of purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Antiserum Preparation
- Antisera to human kappa chains were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Human kappa chains.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
After reconstitution it is recommended to aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/100 | 1/200 | |
Immunohistology - Frozen |
- Flow Cytometry
- Use 50ul of the suggested working dilution to label 106 cells in 100ul.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock |
- RRID
- AB_999298
- UniProt
- P01834
- Entrez Gene
- IGKC
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0006958 complement activation, classical pathway
- GO:0045087 innate immune response
STAR101FB
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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