IRF7 antibody
Rabbit anti Human IRF7 antibody detects human interferon regulatory factor 7 (IRF7), a ~54 kDa transcriptional activator of type I interferons (IFNalpha/beta) in response to local and systemic viral infection. IRF7 is mostly expressed by plasmacytoid dendritic cells (pDC), which differentiate in response to IFNs and virus infection. This then stabilizes the IRF7 protein, in turn leading to increased IFN production.
Multiple IRF7 transcript variants have been identified, although their individual functions have not yet been established.
Multiple IRF7 transcript variants have been identified, although their individual functions have not yet been established.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human IRF7 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide - Immunogen
- 14 amino acid peptide sequence near the centre of human IRF7.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q92985
- Entrez Gene
- IRF7
- GO Terms
- GO:0005886 plasma membrane
- GO:0000122 negative regulation of transcription from RNA polymerase II promoter
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0003677 DNA binding
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003704 specific RNA polymerase II transcription factor activity
- GO:0009615 response to virus
- GO:0005829 cytosol
- GO:0005654 nucleoplasm
- GO:0008063 Toll signaling pathway
- GO:0010008 endosome membrane
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0044419 interspecies interaction between organisms
- GO:0045087 innate immune response
- GO:0060333 interferon-gamma-mediated signaling pathway
- GO:0060337 type I interferon-mediated signaling pathway
- Regulatory
- For research purposes only
Applications of IRF7 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 0.5 | 1.0ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1179 detects a band of approximately 51kDa in 293 whole cell lysate.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Ning, S. et al. (2005) Regulation of the transcriptional activity of the IRF7 promoter by a pathway independent of interferon signaling.
J Biol Chem. 280 (13): 12262-70. -
Prakash, A. & Levy, D.E. (2006) Regulation of IRF7 through cell type-specific protein stability.
Biochem Biophys Res Commun. 342 (1): 50-6.
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