IRF7 antibody

100% Secure


Rabbit anti Human IRF7

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1179 WB 0.1 mg
Rabbit anti Human IRF7 antibody detects human interferon regulatory factor 7 (IRF7), a ~54 kDa transcriptional activator of type I interferons (IFNalpha/beta) in response to local and systemic viral infection. IRF7 is mostly expressed by plasmacytoid dendritic cells (pDC), which differentiate in response to IFNs and virus infection. This then stabilizes the IRF7 protein, in turn leading to increased IFN production.

Multiple IRF7 transcript variants have been identified, although their individual functions have not yet been established.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human IRF7 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
14 amino acid peptide sequence near the centre of human IRF7.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q92985 Related reagents
Entrez Gene
IRF7 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0003677 DNA binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003704 specific RNA polymerase II transcription factor activity
GO:0009615 response to virus
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0008063 Toll signaling pathway
GO:0010008 endosome membrane
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0044419 interspecies interaction between organisms
GO:0045087 innate immune response
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0060337 type I interferon-mediated signaling pathway
Regulatory
For research purposes only

Applications of IRF7 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.5 1.0ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1179 detects a band of approximately 51kDa in 293 whole cell lysate.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Ning, S. et al. (2005) Regulation of the transcriptional activity of the IRF7 promoter by a pathway independent of interferon signaling.
    J Biol Chem. 280 (13): 12262-70.
  2. Prakash, A. & Levy, D.E. (2006) Regulation of IRF7 through cell type-specific protein stability.
    Biochem Biophys Res Commun. 342 (1): 50-6.