IRAK4 antibody

100% Secure


Rabbit anti Human IRAK4 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1662 C WB 0.1 mg
Rabbit anti Human IRAK4 (C-Terminal) antibody detects an epitope within the C-Terminal (CT) region of integrin-1 receptor-associated kinase 4 (IRAK4). IRAK4 is a 460 amino acid ~50 kDa, cytoplasmic protein and is an important mediator in the signal transduction of Toll-like receptor (TLR) and interleukin-1 (IL-1) receptor family members. IRAK4 functions in this signal transduction pathway by binding to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signalling cascades leading to transcriptional up-regulation and mRNA stabilization. IRAK4 is a key activator of TLR-mediated signal transductions and has crucial roles in innate immune responses. It is expressed by the liver, kidneys and skeletal muscle.

Two isoforms of IRAK4 produced by alternative splicing are predicted, Rabbit anti Human IRAK4 (C-Terminal) antibody is expected to recognize both isoforms which differ by an N-terminal deletion.

Mutations in the IRAK4 gene can lead to the development of Recurrent isolated invasive pneumococcal disease 1 (IPD1) characterized by multiple episodes of IPD occurring at least one month apart (Ku et al. 2007). IRAK4 mutation can also lead to the development of IRAK4 deficiency (IRAK4D) demonstrating extracellular pyogenic bacterial and fungal infections in otherwize healthy children (Picard et al. 2003).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to IRAK4 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to amino acids at the carboxy terminus of human IRAK4.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9NWZ3 Related reagents
Entrez Gene
IRAK4 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0000287 magnesium ion binding
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
GO:0008063 Toll signaling pathway
GO:0010008 endosome membrane
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0045087 innate immune response
Regulatory
For research purposes only

Applications of IRAK4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 10ug/ml
Western Blotting 1 2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1662 detects a band of approximately 50kDa in HeLa cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Suzuki, N. et al. (2002) IRAK-4 as the central TIR signaling mediator in innate immunity.
    Trends Immunol. 10: 503-506.
  2. Suzuki, N. and Saito, T. (2006) IRAK-4- a shared NF-kappaB activator in innate and acquired immunity.
    Trends Immunol. 27: 566-572.