IRAK4 antibody
Rabbit anti Human IRAK4 (C-Terminal) antibody detects an epitope within the C-Terminal (CT) region of integrin-1 receptor-associated kinase 4 (IRAK4). IRAK4 is a 460 amino acid ~50 kDa, cytoplasmic protein and is an important mediator in the signal transduction of Toll-like receptor (TLR) and interleukin-1 (IL-1) receptor family members. IRAK4 functions in this signal transduction pathway by binding to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signalling cascades leading to transcriptional up-regulation and mRNA stabilization. IRAK4 is a key activator of TLR-mediated signal transductions and has crucial roles in innate immune responses. It is expressed by the liver, kidneys and skeletal muscle.
Two isoforms of IRAK4 produced by alternative splicing are predicted, Rabbit anti Human IRAK4 (C-Terminal) antibody is expected to recognize both isoforms which differ by an N-terminal deletion.
Mutations in the IRAK4 gene can lead to the development of Recurrent isolated invasive pneumococcal disease 1 (IPD1) characterized by multiple episodes of IPD occurring at least one month apart (Ku et al. 2007). IRAK4 mutation can also lead to the development of IRAK4 deficiency (IRAK4D) demonstrating extracellular pyogenic bacterial and fungal infections in otherwize healthy children (Picard et al. 2003).
Two isoforms of IRAK4 produced by alternative splicing are predicted, Rabbit anti Human IRAK4 (C-Terminal) antibody is expected to recognize both isoforms which differ by an N-terminal deletion.
Mutations in the IRAK4 gene can lead to the development of Recurrent isolated invasive pneumococcal disease 1 (IPD1) characterized by multiple episodes of IPD occurring at least one month apart (Ku et al. 2007). IRAK4 mutation can also lead to the development of IRAK4 deficiency (IRAK4D) demonstrating extracellular pyogenic bacterial and fungal infections in otherwize healthy children (Picard et al. 2003).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to IRAK4 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- A peptide corresponding to amino acids at the carboxy terminus of human IRAK4.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9NWZ3
- Entrez Gene
- IRAK4
- GO Terms
- GO:0005886 plasma membrane
- GO:0000287 magnesium ion binding
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0005829 cytosol
- GO:0004674 protein serine/threonine kinase activity
- GO:0005524 ATP binding
- GO:0006468 protein phosphorylation
- GO:0008063 Toll signaling pathway
- GO:0010008 endosome membrane
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0045087 innate immune response
- Regulatory
- For research purposes only
Applications of IRAK4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | 10ug/ml | ||
| Western Blotting | 1 | 2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1662 detects a band of approximately 50kDa in HeLa cell lysates.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Suzuki, N. et al. (2002) IRAK-4 as the central TIR signaling mediator in innate immunity.
Trends Immunol. 10: 503-506. -
Suzuki, N. and Saito, T. (2006) IRAK-4- a shared NF-kappaB activator in innate and acquired immunity.
Trends Immunol. 27: 566-572.
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