IRAK1 antibody

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Rabbit anti Human IRAK1 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP445 C IP WB 0.1 mg
Rabbit anti Human IRAK1 antibody recognizes human Interleukin-1 receptor-associated kinase 1 (IRAK1) an ~80 kDa serine/threonine protein kinase designated IRAK containing a single protein kinase domain and a single death domain. IRAK is associated with IL-1 receptor subunits, and mediates a signalling cascade leading to NF-kB activation after IL-1 binding (Burns et al. 2000).Multiple isoforms of IRAK1 generated by alternative splicing have been described. Isoforms 1 and 2 are ubiquitously expressed (Jensen et al. 2001). Rabbit anti Human IRAK1 antibody recognizes isoform 1 but reactivity with other isoforms having partial deletions of the carboxy-terminal region has not been assessed.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human IRAK were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
Peptide from C terminus of human IRAK
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P51617 Related reagents
Entrez Gene
IRAK1 Related reagents
GO Terms
GO:0000187 activation of MAPK activity
GO:0005515 protein binding
GO:0001959 regulation of cytokine-mediated signaling pathway
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005829 cytosol
GO:0004704 NF-kappaB-inducing kinase activity
GO:0004842 ubiquitin-protein ligase activity
GO:0007178 transmembrane receptor protein serine/threonine kinase signaling pathway
GO:0005524 ATP binding
GO:0006916 anti-apoptosis
GO:0007250 activation of NF-kappaB-inducing kinase activity
GO:0007254 JNK cascade
GO:0008063 Toll signaling pathway
GO:0010008 endosome membrane
GO:0016563 transcription activator activity
GO:0031663 lipopolysaccharide-mediated signaling pathway
GO:0032088 negative regulation of NF-kappaB transcription factor activity
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0042803 protein homodimerization activity
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0045087 innate immune response
GO:0045323 interleukin-1 receptor complex
GO:0046777 protein autophosphorylation
GO:0046982 protein heterodimerization activity
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051259 protein oligomerization
GO:0051403 stress-activated MAPK cascade
GO:0070498 interleukin-1-mediated signaling pathway
GO:0070555 response to interleukin-1
Regulatory
For research purposes only

Applications of IRAK1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunoprecipitation 2-4ug/sample
Western Blotting 1/1000 1/2000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Cao, Z. et al. (1996) IRAK: a kinase associated with the interleukin-1 receptor.
    Science. 271 (5252): 1128-31.