IRAK1 antibody
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WB IC ResetRabbit anti Human IRAK1 (C-Terminal)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IRAK1
- Region
- (C-TERMINAL)
Antibody Quality and 
Unrivaled 
| Rabbit anti Human IRAK1 antibody recognizes human Interleukin-1 receptor-associated kinase 1 (IRAK1) an ~80 kDa serine/threonine protein kinase designated IRAK containing a single protein kinase domain and a single death domain. IRAK is associated with IL-1 receptor subunits, and mediates a signalling cascade leading to NF-kB activation after IL-1 binding (Burns et al. 2000).Multiple isoforms of IRAK1 generated by alternative splicing have been described. Isoforms 1 and 2 are ubiquitously expressed (Jensen et al. 2001). Rabbit anti Human IRAK1 antibody recognizes isoform 1 but reactivity with other isoforms having partial deletions of the carboxy-terminal region has not been assessed. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human IRAK were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide - Immunogen
- Peptide from C terminus of human IRAK
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen |  |
||
| Immunoprecipitation | |
2-4ug/sample | |
| Western Blotting | |
1/1000 | 1/2000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Further Reading
-
Cao, Z. et al. (1996) IRAK: a kinase associated with the interleukin-1 receptor.
Science. 271 (5252): 1128-31.
- RRID
- AB_322748
- UniProt
- P51617
- Entrez Gene
- IRAK1
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0005515 protein binding
- GO:0001959 regulation of cytokine-mediated signaling pathway
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0005829 cytosol
- GO:0004704 NF-kappaB-inducing kinase activity
- GO:0004842 ubiquitin-protein ligase activity
- GO:0007178 transmembrane receptor protein serine/threonine kinase signaling pathway
- View More GO Terms
- GO:0005524 ATP binding
- GO:0006916 anti-apoptosis
- GO:0007250 activation of NF-kappaB-inducing kinase activity
- GO:0007254 JNK cascade
- GO:0008063 Toll signaling pathway
- GO:0010008 endosome membrane
- GO:0016563 transcription activator activity
- GO:0031663 lipopolysaccharide-mediated signaling pathway
- GO:0032088 negative regulation of NF-kappaB transcription factor activity
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0042803 protein homodimerization activity
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0045087 innate immune response
- GO:0045323 interleukin-1 receptor complex
- GO:0046777 protein autophosphorylation
- GO:0046982 protein heterodimerization activity
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0051259 protein oligomerization
- GO:0051403 stress-activated MAPK cascade
- GO:0070498 interleukin-1-mediated signaling pathway
- GO:0070555 response to interleukin-1
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