Rabbit anti Human IRAK1 antibody
recognizes human Interleukin-1 receptor-associated kinase 1 (IRAK1) an ~80 kDa serine/threonine protein kinase designated IRAK containing a single protein kinase domain
and a single death domain
. IRAK is associated with IL-1 receptor subunits, and mediates a signalling cascade leading to NF-kB activation after IL-1 binding (Burns et al. 2000
).Multiple isoforms of IRAK1 generated by alternative splicing have been described. Isoforms 1 and 2 are ubiquitously expressed (Jensen et al. 2001
). Rabbit anti Human IRAK1 antibody recognizes isoform 1 but reactivity with other isoforms having partial deletions of the carboxy-terminal region has not been assessed.
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human IRAK were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Peptide from C terminus of human IRAK
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
activation of MAPK activity
regulation of cytokine-mediated signaling pathway
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
NF-kappaB-inducing kinase activity
ubiquitin-protein ligase activity
transmembrane receptor protein serine/threonine kinase signaling pathway
activation of NF-kappaB-inducing kinase activity
Toll signaling pathway
transcription activator activity
lipopolysaccharide-mediated signaling pathway
negative regulation of NF-kappaB transcription factor activity
toll-like receptor 1 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
protein homodimerization activity
positive regulation of I-kappaB kinase/NF-kappaB cascade
innate immune response
interleukin-1 receptor complex
protein heterodimerization activity
nerve growth factor receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
stress-activated MAPK cascade
interleukin-1-mediated signaling pathway
response to interleukin-1
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IRAK1 antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Cao, Z. et al. (1996) IRAK: a kinase associated with the interleukin-1 receptor.
Science. 271 (5252): 1128-31.