IRAK1 antibody
Rabbit anti Human IRAK1 antibody recognizes human Interleukin-1 receptor-associated kinase 1 (IRAK1) an ~80 kDa serine/threonine protein kinase designated IRAK containing a single protein kinase domain and a single death domain. IRAK is associated with IL-1 receptor subunits, and mediates a signalling cascade leading to NF-kB activation after IL-1 binding (Burns et al. 2000).Multiple isoforms of IRAK1 generated by alternative splicing have been described. Isoforms 1 and 2 are ubiquitously expressed (Jensen et al. 2001). Rabbit anti Human IRAK1 antibody recognizes isoform 1 but reactivity with other isoforms having partial deletions of the carboxy-terminal region has not been assessed.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human IRAK were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide - Immunogen
- Peptide from C terminus of human IRAK
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P51617
- Entrez Gene
- IRAK1
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0005515 protein binding
- GO:0001959 regulation of cytokine-mediated signaling pathway
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0005829 cytosol
- GO:0004704 NF-kappaB-inducing kinase activity
- GO:0004842 ubiquitin-protein ligase activity
- GO:0007178 transmembrane receptor protein serine/threonine kinase signaling pathway
- GO:0005524 ATP binding
- GO:0006916 anti-apoptosis
- GO:0007250 activation of NF-kappaB-inducing kinase activity
- GO:0007254 JNK cascade
- GO:0008063 Toll signaling pathway
- GO:0010008 endosome membrane
- GO:0016563 transcription activator activity
- GO:0031663 lipopolysaccharide-mediated signaling pathway
- GO:0032088 negative regulation of NF-kappaB transcription factor activity
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0042803 protein homodimerization activity
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0045087 innate immune response
- GO:0045323 interleukin-1 receptor complex
- GO:0046777 protein autophosphorylation
- GO:0046982 protein heterodimerization activity
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0051259 protein oligomerization
- GO:0051403 stress-activated MAPK cascade
- GO:0070498 interleukin-1-mediated signaling pathway
- GO:0070555 response to interleukin-1
- Regulatory
- For research purposes only
Applications of IRAK1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | |||
| Immunoprecipitation | 2-4ug/sample | ||
| Western Blotting | 1/1000 | 1/2000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
|
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Cao, Z. et al. (1996) IRAK: a kinase associated with the interleukin-1 receptor.
Science. 271 (5252): 1128-31.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
