IRAK1 antibody

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Anti Human IRAK1 (C-Terminal) Antibody thumbnail image 1

Anti Human IRAK1 (C-Terminal) Antibody thumbnail image 2

Anti Human IRAK1 (C-Terminal) Antibody gallery image 1 — Western Blot analysis of THP-1 (THP) and HeLa (HL) cell lysates probed with Rabbit anti Human IRAK-1 (**AHP445**)

Anti Human IRAK1 (C-Terminal) Antibody gallery image 2 — Immunocytochemical staining of HeLa cells with Rabbit anti Human IRAK-1 (AHP445)

Rabbit anti Human IRAK1 (C-Terminal)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP445	C IP WB			0.1 mg

Rabbit anti Human IRAK1 antibody recognizes human Interleukin-1 receptor-associated kinase 1 (IRAK1) an ~80 kDa serine/threonine protein kinase designated IRAK containing a single protein kinase domain and a single death domain. IRAK is associated with IL-1 receptor subunits, and mediates a signalling cascade leading to NF-kB activation after IL-1 binding (Burns et al. 2000).Multiple isoforms of IRAK1 generated by alternative splicing have been described. Isoforms 1 and 2 are ubiquitously expressed (Jensen et al. 2001). Rabbit anti Human IRAK1 antibody recognizes isoform 1 but reactivity with other isoforms having partial deletions of the carboxy-terminal region has not been assessed.

Product Details

Target Species

Human

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to human IRAK were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.02%

Sodium Azide

Immunogen

Peptide from C terminus of human IRAK

Approx. Protein Concentrations

IgG concentration 0.5 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P51617 Related reagents
Entrez Gene: IRAK1 Related reagents
GO Terms: GO:0000187 activation of MAPK activity; GO:0005515 protein binding; GO:0001959 regulation of cytokine-mediated signaling pathway; GO:0002755 MyD88-dependent toll-like receptor signaling pathway; GO:0002756 MyD88-independent toll-like receptor signaling pathway; GO:0005829 cytosol; GO:0004704 NF-kappaB-inducing kinase activity; GO:0004842 ubiquitin-protein ligase activity; GO:0007178 transmembrane receptor protein serine/threonine kinase signaling pathway; GO:0005524 ATP binding; GO:0006916 anti-apoptosis; GO:0007250 activation of NF-kappaB-inducing kinase activity; GO:0007254 JNK cascade; GO:0008063 Toll signaling pathway; GO:0010008 endosome membrane; GO:0016563 transcription activator activity; GO:0031663 lipopolysaccharide-mediated signaling pathway; GO:0032088 negative regulation of NF-kappaB transcription factor activity; GO:0034130 toll-like receptor 1 signaling pathway; GO:0034134 toll-like receptor 2 signaling pathway; GO:0034138 toll-like receptor 3 signaling pathway; GO:0034142 toll-like receptor 4 signaling pathway; GO:0042803 protein homodimerization activity; GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade; GO:0045087 innate immune response; GO:0045323 interleukin-1 receptor complex; GO:0046777 protein autophosphorylation; GO:0046982 protein heterodimerization activity; GO:0048011 nerve growth factor receptor signaling pathway; GO:0051092 positive regulation of NF-kappaB transcription factor activity; GO:0051259 protein oligomerization; GO:0051403 stress-activated MAPK cascade; GO:0070498 interleukin-1-mediated signaling pathway; GO:0070555 response to interleukin-1
Regulatory: For research purposes only

Applications of IRAK1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Immunohistology - Frozen
Immunoprecipitation		2-4ug/sample
Western Blotting	1/1000	1/2000

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Sheep anti Rabbit IgG:Biotin	2AB02B	1 ml	C E P WB
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	1 mg	E WB
Goat anti Rabbit IgG (Fc):FITC	STAR121F	1 mg	F
Goat anti Rabbit IgG (Fc):HRP	STAR121P	1 mg	E WB
Goat anti Rabbit IgG (H/L):HRP	STAR124P	1 mg	C E WB
Sheep anti Rabbit IgG:FITC	STAR34B	1 mg	C F
Sheep anti Rabbit IgG:RPE	STAR35A	1 ml	F
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:DyLight®549	STAR36D549GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®649	STAR36D649GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	0.1 mg	F IF WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
TidyBlot™ Western Blot Detection Reagent:HRP	STAR209P	0.5 ml	WB*