Rabbit anti Human IRAK1 antibody
recognizes human Interleukin-1 receptor-associated kinase 1 (IRAK1) an ~80 kDa serine/threonine protein kinase designated IRAK containing a single protein kinase domain
and a single death domain
. IRAK is associated with IL-1 receptor subunits, and mediates a signalling cascade leading to NF-kB activation after IL-1 binding (Burns et al. 2000
).Multiple isoforms of IRAK1 generated by alternative splicing have been described. Isoforms 1 and 2 are ubiquitously expressed (Jensen et al. 2001
). Rabbit anti Human IRAK1 antibody recognizes isoform 1 but reactivity with other isoforms having partial deletions of the carboxy-terminal region has not been assessed.
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human IRAK were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Peptide from C terminus of human IRAK
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
activation of MAPK activity
regulation of cytokine-mediated signaling pathway
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
NF-kappaB-inducing kinase activity
ubiquitin-protein ligase activity
transmembrane receptor protein serine/threonine kinase signaling pathway
activation of NF-kappaB-inducing kinase activity
Toll signaling pathway
transcription activator activity
lipopolysaccharide-mediated signaling pathway
negative regulation of NF-kappaB transcription factor activity
toll-like receptor 1 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
protein homodimerization activity
positive regulation of I-kappaB kinase/NF-kappaB cascade
innate immune response
interleukin-1 receptor complex
protein heterodimerization activity
nerve growth factor receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
stress-activated MAPK cascade
interleukin-1-mediated signaling pathway
response to interleukin-1
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IRAK1 antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Cao, Z. et al. (1996) IRAK: a kinase associated with the interleukin-1 receptor.
Science. 271 (5252): 1128-31.
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