IL-8 antibody
Rabbit anti Human Interleukin-8
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IL-8
Antibody Quality and Performance Guarantee
| Rabbit anti Human Interleukin-8 antibody recognizes human IL-8 (Interleukin-8), a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8, secreted by endothelial cells and monocytes, which acts as a major chemoattractant for neutrophils and also T cells and basophils. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-Terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1(NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antisera to human IL-8 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human IL-8.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0ug/ml |
| Functional Assays | |
2.0 | 3.0ug/ml |
| Immunofluorescence | |
||
| Immunohistology - Paraffin 1 | |
0.25 | 1.0ug/ml |
| Western Blotting | |
0.1 | 0.2ug/ml |
- 1> This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
- ELISA
- Rabbit anti human IL-8 antibody (AHP781) may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with biotinylated human IL-8 antibody as the detection reagent .
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| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
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| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
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References for IL-8 antibody
-
Vazquez, G.R. et al. (2018) Adipocytes Promote Early Steps of Breast Cancer Cell Dissemination via Interleukin-8.
Front Immunol. 9: 1767. -
Fu, X. et al. (2023) High FOXA1 levels induce ER transcriptional reprogramming, a pro-metastatic secretome, and metastasis in endocrine-resistant breast cancer.
Cell Rep. 42 (8): 112821.
Further Reading
-
Mukaida, N. et al. (1989) Genomic structure of the human monocyte-derived neutrophil chemotactic factor IL-8.
J Immunol. 143 (4): 1366-71.
- Synonyms
- CXCL8
- RRID
- AB_2264854
- UniProt
- P10145
- Entrez Gene
- IL8
View more products with IL-8 specificity
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