IL-8 antibody

Rabbit anti Human Interleukin-8

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IL-8

Product Code Applications Pack Size List Price Your Price Qty
AHP781
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN IF P * WB 0.1 mg loader
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Search for Batch Specific Datasheets

Rabbit anti Human Interleukin-8 antibody recognizes human IL-8 (Interleukin-8), a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8, secreted by endothelial cells and monocytes, which acts as a major chemoattractant for neutrophils and also T cells and basophils.

IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-Terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1(NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C.

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to human IL-8 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant human IL-8 (PHP055)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Functional Assays 2.0 3.0ug/ml
Immunofluorescence
Immunohistology - Paraffin 1 0.25 1.0ug/ml
Western Blotting 0.1 0.2ug/ml
  1. 1 This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.

ELISA
This purified human IL-8 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a biotinylated human IL-8 antibody (AHP781B) as the detection reagent and recombinant human IL-8 (PHP055) as the standard.
Western Blotting
This purified human IL-8 antibody may used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human IL-8 (PHP055) as the positive control.

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References for IL-8 antibody

  1. Rodriguez, G.V. et al. (2018) Adipocytes Promote Early Steps of Breast Cancer Cell Dissemination via Interleukin-8
    Frontiers in Immunology. 9 [Epub ahead of print].

Further Reading

  1. Mukaida, N. et al. (1989) Genomic structure of the human monocyte-derived neutrophil chemotactic factor IL-8.
    J Immunol. 143 (4): 1366-71.

Synonyms
CXCL8
RRID
AB_2264854
UniProt
P10145
Entrez Gene
IL8
View more products with IL-8 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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