IL-32 antibody

Rabbit anti Human Interleukin-32

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IL-32

Product Code Applications Pack Size List Price Your Price Qty
AHP1206
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SDS Safety Datasheet SDS
WB 0.1 mg loader
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Rabbit anti Human interleukin-32 antibody recognises IL-32, a ~26 kDa cytokine, thought to play a role in innate and adaptive immune responses. IL-32 is produced by mitogen-activated lymphocytes, interferon-gamma activated epithelial cells and IL-12, IL-18, IL-32-activated NK cells. It is expressed at elevated levels after the activation of T-cells by mitogens or the activation of NK cells by IL-2. IL-32 activates NF-kappa-B and p38 MAPK cytokine signal pathways. TNF-alpha, IL-1beta, IL-6 and 2 C-X-C chemokine family members, all involved in autoimmune and inflammatory diseases, including rheumatoid arthritis are induced by IL-32.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Antisera to human IL-32 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
A 16 amino acid peptide located near the centre of human IL-32.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 5 10ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1206 detects a band/s of approximately 26kDa in jurkat cell lysate.

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Further Reading

  1. Conti, P. (2007) Modulation of autoimmunity by the latest interleukins (with special emphasis on IL-32).
    Autoimmun Rev. 6:131-7.
  2. Chen, Q. (2006) The newest interleukins: recent additions to the ever-growing cytokine family.
    Vitam Horm. 74:207-28.
  3. Kim, S. (2005) Interleukin-32: a cytokine and inducer of TNFalpha.
    Immunity. 22:131-42.
  4. Cagnard, N. (2005) Interleukin-32, CCL2, PF4F1 and GFD10 are the only cytokine/chemokine genes differentially expressed by in vitro cultured rheumatoid and osteoarthritis fibroblast-like synoviocytes.
    Eur Cytokine Netw. 16:289-92.

RRID
AB_844624
UniProt
P24001
Entrez Gene
IL32
GO Terms
GO:0006952 defense response
GO:0007155 cell adhesion
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006955 immune response
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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