IL-16 antibody

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Rabbit anti Human Interleukin-16

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP454G E FN WB 0.1 mg
Rabbit anti Human Interleukin-16 antibody recognizes human IL-16 (Interleukin-16), originally known as leucocyte chemoattractant factor (LCF), an immunomodulatory cytokine which induces the chemotaxis of CD4 expressing T cells, eosinophils and monocytes and is produced constitutively by T cells, eosinophils, dendritic cells, fibroblasts and neuronal cells.

The biologically active form of IL-16 is generated by enzymatic cleavage of the IL-16 precursor (pro-IL16), producing a peptide corresponding to the 121 amino acid C-Terminal portion. Further properties attributed to IL-16 include the induction of MHC class II and IL-2 receptor (IL-2R) expression, and several studies have indicated a role for IL-16 in inflammatory processes.

Product Details

Target Species
Human
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Antiserum Preparation
Antisera to human IL-16 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by antigen affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant human IL-16
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. For long term storage the addition of 0.09% Sodium Azide is recommended.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
Q14005 Related reagents
Entrez Gene
IL16 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006350 transcription
GO:0006955 immune response
GO:0044419 interspecies interaction between organisms
GO:0045449 regulation of transcription
Regulatory
For research purposes only

Applications of IL-16 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 ug/ml
Functional Assays 12 70ng/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
For functional studies do not add Azide.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for IL-16 antibody

  1. Cruikshank, W.W. et al. (1994) Molecular and functional analysis of a lymphocyte chemoattractant factor: association of biologic function with CD4 expression.
    Proc Natl Acad Sci U S A. 91 (11): 5109-13.
  2. Cruikshank, W.W. et al. (2000) Interleukin-16.
    J Leukoc Biol. 67 (6): 757-66.