IL-1 Alpha antibody

Rabbit anti Human Interleukin-1 Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IL-1 Alpha

Product Code Applications Pack Size List Price Your Price Qty
AHP281G
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SDS Safety Datasheet SDS
E FN P * WB 0.1 mg loader
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loader
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Rabbit anti Human Interleukin-1 alpha antibody recognizes human IL-1 alpha (Interleukin-1 alpha), an 18kDa multi-functional pro-inflammatory cytokine which belongs to the IL-1 family of proteins, and is produced by a variety of cells including activated macrophages, monocytes and keratinocytes.

IL-1 alpha and IL-1 beta have been identified as two distinct IL-1 proteins which bind to the same type 1 Interleukin 1 receptor (IL1R1). Biological properties of IL-1 alpha include stimulation of thymocyte proliferation by induction of IL-2 release, B-cell proliferation and maturation, and fibroblast growth factor activity.

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1 ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Antiserum Preparation
Antisera to human IL-1 alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant human IL-1alpha.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml
Functional Assays 1.0 2.0ug/ml
Immunohistology - Paraffin 1
Western Blotting 0.1 0.2ug/ml
  1. 1 This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for IL-1 Alpha antibody

  1. Gubler, U. et al. (1986) Recombinant human interleukin 1 alpha: purification and biological characterization.
    J Immunol. 136 (7): 2492-7.
  2. Kilian, P.L. et al. (1986) Interleukin 1 alpha and interleukin 1 beta bind to the same receptor on T cells.
    J Immunol. 136 (12): 4509-14.
  3. Henry CM et al. (2016) Neutrophil-Derived Proteases Escalate Inflammation through Activation of IL-36 Family Cytokines.
    Cell Rep. 14 (4): 708-22.
  4. Frezza, V. et al. (2022) IL-1α and IL-36 Family Cytokines Can Undergo Processing and Activation by Diverse Allergen-Associated Proteases.
    Front Immunol. 13: 879029.

RRID
AB_2124195
UniProt
P01583
Entrez Gene
IL1A
GO Terms
GO:0001660 fever generation
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0005125 cytokine activity
GO:0005149 interleukin-1 receptor binding
GO:0005507 copper ion binding
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0006955 immune response
GO:0008283 cell proliferation
GO:0008285 negative regulation of cell proliferation
GO:0010575 positive regulation vascular endothelial growth factor production
GO:0019221 cytokine-mediated signaling pathway
GO:0034605 cellular response to heat
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0046688 response to copper ion
GO:0045766 positive regulation of angiogenesis
GO:0045840 positive regulation of mitosis
GO:0050715 positive regulation of cytokine secretion
GO:0051781 positive regulation of cell division
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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