IgG antibody
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Goat anti Human IgG (Fc) antibody conjugated to Alkaline Phosphatase recognizes the Fc frafment of the human IgG heavy chain.
Immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, human IgG, human IgG (Fc) and human serum. No reaction was observed against human IgG F(ab')2.
Immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, human IgG, human IgG (Fc) and human serum. No reaction was observed against human IgG F(ab')2.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Preparation
- Purified IgG prepared from monospecific antiserum by immunoaffinity chromatography
- Buffer Solution
- TRIS buffered saline
0.001M Magnesium Chloride
0.0001M Zinc Chloride - Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
50% Glycerol
1% Bovine Serum Albumin - Immunogen
- Human IgG Fc fragment.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01860
- P01857
- P01859
- P01861
- Entrez Gene
- IGHG1
- IGHG2
- IGHG3
- IGHG4
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0005624 membrane fraction
- GO:0006955 immune response
- Regulatory
- For research purposes only
Applications of IgG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/5,000 | 1/20,000 | |
| Immunohistology - Frozen | 1/200 | 1/1,000 | |
| Western Blotting | 1/500 | 1/2,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for IgG antibody
-
Zeman, D. et al. (2012) Detection of oligoclonal IgG kappa and IgG lambda bands in cerebrospinal fluid and serum with Hevylite™ antibodies. comparison with the free light chain oligoclonal pattern.
Fluids Barriers CNS. 9: 5.
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