IgG antibody

Goat anti Human IgG (Fc):Alk. Phos.

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(Fc)

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5211-8104
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Goat anti Human IgG (Fc) antibody conjugated to Alkaline Phosphatase recognizes the Fc frafment of the human IgG heavy chain.

Immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, human IgG, human IgG (Fc) and human serum. No reaction was observed against human IgG F(ab')2.

Target Species
Human
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Preparation
Purified IgG prepared from monospecific antiserum by immunoaffinity chromatography
Buffer Solution
TRIS buffered saline
0.001M Magnesium Chloride
0.0001M Zinc Chloride
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
50% Glycerol
1% Bovine Serum Albumin
Immunogen
Human IgG Fc fragment.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5,000 1/20,000
Immunohistology - Frozen 1/200 1/1,000
Western Blotting 1/500 1/2,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

References for IgG antibody

  1. Zeman, D. et al. (2012) Detection of oligoclonal IgG kappa and IgG lambda bands in cerebrospinal fluid and serum with Hevylite™ antibodies. comparison with the free light chain oligoclonal pattern.
    Fluids Barriers CNS. 9: 5.

Immunoblotting

RRID
AB_619807
UniProt
P01860
P01857
P01859
P01861
Entrez Gene
IGHG1
IGHG2
IGHG3
IGHG4
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0005624 membrane fraction
GO:0006955 immune response
View more products with IGG specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products