IgG antibody
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Goat anti Human IgG (Fc):Alk. Phos.
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG
- Region
- (Fc)
Antibody Quality and Performance Guarantee
| Goat anti Human IgG (Fc) antibody conjugated to Alkaline Phosphatase recognizes the Fc frafment of the human IgG heavy chain. Immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, human IgG, human IgG (Fc) and human serum. No reaction was observed against human IgG F(ab')2. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Preparation
- Purified IgG prepared from monospecific antiserum by immunoaffinity chromatography
- Buffer Solution
- 0.05M TRIS Chloride
0.15M NaCl
0.001M MgCl2
0.0001M ZnCl2
50% (v/v) Glycerol; pH8.0 - Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Human IgG Fc fragment.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted.
This product should be stored undiluted.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/5,000 | 1/20,000 |
| Immunohistology - Frozen | |
1/200 | 1/1,000 |
| Western Blotting | |
1/500 | 1/2,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
References for IgG antibody
-
Zeman, D. et al. (2012) Detection of oligoclonal IgG kappa and IgG lambda bands in cerebrospinal fluid and serum with Hevylite™ antibodies. comparison with the free light chain oligoclonal pattern.
Fluids Barriers CNS. 9: 5.
- RRID
- AB_619807
- UniProt
- P01860
- P01857
- P01859
- P01861
- Entrez Gene
- IGHG1
- IGHG2
- IGHG3
- IGHG4
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0005624 membrane fraction
- GO:0006955 immune response
5211-8104
151212
156401
168360
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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