IgG antibody
Horseradish peroxidase conjugated Goat anti Human IgG antibody (5172-2504) used as the detection reagent in a serodiagnostic EISA for human brucellosis.
Image caption:
A Dot plot result of ELISA assays (LPS vs. rOMP19 antigen). B ROC analysis of rOMP19 ELISA compared with Rose Bengal test
From: Golchin M, Mollayi S, Mohammadi E, Eskandarzade N.
Serodiagnosis of human brucellosis by an indirect ELISA test using recombinant outer membrane protein 19 kDa (rOMP19) as an antigen.
BMC Biotechnol. 2023 Oct 24;23(1):46.
doi: 10.1186/s12896-023-00817-2.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Human IgG (H/L):HRP
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG
- Region
- (H/L)
Antibody Quality and Performance Guarantee
| Goat anti Human IgG antibody is specific for Human IgG. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, human IgG and human serum. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilized
- Reconstitution
- Reconstitute with 1ml deionised water. For extended storage aliquot and freeze.
- Antiserum Preparation
- Goat anti Human IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography with human IgG coupled to agarose beads followed by solid phase adsorbtion to remove unwanted cross reactivity.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.01% Gentamicin sulphate 1% Bovine Serum Albumin - Immunogen
- Human IgG, whole molecule.
- Approx. Protein Concentrations
- Ig concentration 2.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/25,000 | 1/100,000 |
| Immunohistology - Frozen | |
1/1,000 | 1/5,000 |
| Western Blotting | |
1/2,000 | 1/10,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | AbGUARD® HRP Stabilizer Plus | ||||||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | AbGUARD® HRP Stabilizer Plus | ||||||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | AbGUARD® HRP Stabilizer Plus | ||||||
| TMB Signal+ | BUF054A | E | 100 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TMB Signal+ | ||||||
| TMB Core | BUF056A | E | 100 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TMB Core | ||||||
| TMB Core+ | BUF062A | E | 100 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TMB Core+ | ||||||
References for IgG antibody
-
Golchin, M. et al. (2023) Serodiagnosis of human brucellosis by an indirect ELISA test using recombinant outer membrane protein 19 kDa (rOMP19) as an antigen.
BMC Biotechnol. 23 (1): 46.
- RRID
- AB_619882
- UniProt
- P01859
- P01860
- P01861
- P01834
- P01857
- P0CG04
- Entrez Gene
- IGKC
- IGHG1
- IGHG2
- IGHG3
- IGHG4
- IGLV2-14
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0006958 complement activation, classical pathway
- GO:0045087 innate immune response
- GO:0005624 membrane fraction
- GO:0006955 immune response
5172-2504
148712
154269
155639
160652
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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