IgG antibody
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Goat anti Human IgG antibody is specific for Human IgG. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, human IgG and human serum.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilised
- Reconstitution
- Reconstitute with 1ml deionised water. For extended storage aliquot and freeze.
- Antiserum Preparation
- Goat anti Human IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography with human IgG coupled to agarose beads followed by solid phase adsorbtion to remove unwanted cross reactivity.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.01% Gentamicin sulphate 1% Bovine Serum Albumin - Immunogen
- Human IgG, whole molecule.
- Approx. Protein Concentrations
- Ig concentration 2.0 mg/ml
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- P01859 Related reagents
- P01860 Related reagents
- P01861 Related reagents
- P01834 Related reagents
- P01857 Related reagents
- P0CG04 Related reagents
- Entrez Gene
- IGKC Related reagents
- IGHG1 Related reagents
- IGHG2 Related reagents
- IGHG3 Related reagents
- IGHG4 Related reagents
- IGLV2-14 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0006958 complement activation, classical pathway
- GO:0045087 innate immune response
- GO:0005624 membrane fraction
- GO:0006955 immune response
- Regulatory
- For research purposes only
Applications of IgG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/25,000 | 1/100,000 | |
| Immunohistology - Frozen | 1/1,000 | 1/5,000 | |
| Western Blotting | 1/2,000 | 1/10,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | 100 ml | C E P WB | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | 500 ml | C E P WB | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | 1000 ml | C E P WB | ||
| TMB Signal+ | BUF054A | 100 ml | E | ||
| TMB Core | BUF056A | 100 ml | E | ||
| TMB Core+ | BUF062A | 100 ml | E |
