IgG antibody

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Goat anti Human IgG (H/L):HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
5172-2504 C E WB 2 mg
Goat anti Human IgG antibody is specific for Human IgG. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, human IgG and human serum.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilised
Reconstitution
Reconstitute with 1ml deionised water. For extended storage aliquot and freeze.
Antiserum Preparation
Goat anti Human IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography with human IgG coupled to agarose beads followed by solid phase adsorbtion to remove unwanted cross reactivity.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Gentamicin sulphate
1%Bovine Serum Albumin
Immunogen
Human IgG, whole molecule.
Approx. Protein Concentrations
Ig concentration 2.0 mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P01859 Related reagents
P01860 Related reagents
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P0CG04 Related reagents
Entrez Gene
IGKC Related reagents
IGHG1 Related reagents
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IGHG3 Related reagents
IGHG4 Related reagents
IGLV2-14 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
GO:0005624 membrane fraction
GO:0006955 immune response
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/25,000 1/100,000
Immunohistology - Frozen 1/1,000 1/5,000
Western Blotting 1/2,000 1/10,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E