IgA antibody

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Goat F(ab')2 anti Human IgA:FITC

Goat F(ab')2 anti Human IgA

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
STAR142F C F IF 0.5 mg
STAR142 E 0.5 mg
Goat F(ab')2 anti Human IgA antibody recognizes the heavy chain of human IgA and has been cross absorbed against human IgM, IgD and IgG. Goat F(ab')2 anti Human IgA antibody might cross react with IgA from other species.

Product Details

Target Species
Human
Product Form
F(ab')2 fragments of purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
F(ab')2 fragments of purified IgG - liquid
Antiserum Preparation
Antisera to human IgA were raised by repeated immunisation of goats with purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. F(ab)2 fragments were prepared by pepsin digestion.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Borate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Human IgA
Approx. Protein Concentrations
F(ab')2 concentration 0.5mg/ml
Approx. Protein Concentrations
F(ab')2 concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01877 Related reagents
P01876 Related reagents
Entrez Gene
IGHA1 Related reagents
IGHA2 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0006955 immune response
GO:0018298 protein-chromophore linkage
GO:0005624 membrane fraction
Regulatory
For research purposes only

Applications of IgA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Immunofluorescence Neat 1/10
Immunohistology - Frozen
ELISA
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit F(ab')2 anti Goat IgG (H/L):Alk. Phos. 602004 1 ml C E IB WB
Donkey anti Goat IgG (H/L):Alk. Phos. (Rat/Mouse Adsorbed) 642004 1 ml C E
Donkey anti Goat IgG (H/L):HRP (Rat/Mouse Adsorbed) 642005 1 ml C E
Donkey anti Goat IgG (H/L):Biotin (Rat/Mouse Adsorbed) 642008 1 mg C E

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Product Specific References

References for IgA antibody

  1. Lu, D.R. et al. (2014) Identifying functional anti-Staphylococcus aureus antibodies by sequencing antibody repertoires of patient plasmablasts.
    Clin Immunol. 152 (1-2): 77-89.