IFN Beta antibody

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Anti IFN Beta Antibody thumbnail image 2

Anti IFN Beta Antibody gallery image 1 — Western blot analysis of whole cell lysate from A-20 mouse B-cell lymphoma probed with Rabbit anti Human IFN beta (**AHP1435**).

Anti IFN Beta Antibody gallery image 2 — Formalin fixed, paraffin embedded human liver stained with Rabbit anti Human IFN beta (**AHP1435**) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0).

Rabbit anti IFN Beta

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP1435	P* WB			0.1 mg

Rabbit anti Human interferon beta antibody recognizes interferon beta (IFN-beta), a 22 kDa member of the type I alpha/beta interferon family. IFN beta, initially described as fibroblast interferon, is produced predominantly by fibroblasts and some epithelial cells. Type 1 interferons are produced primarily in response to viral infections as part of the host immune response and can also inhibit the development of tumors. IFN-beta is involved in the regulation of non-specific humoral immune responses and responses against viral infections.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to human IFN Beta were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.02%

Sodium Azide

Immunogen

A 17 amino acid peptide located near the centre of human IFN Beta.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P01574 Related reagents
Entrez Gene: IFNB1 Related reagents
GO Terms: GO:0003714 transcription corepressor activity; GO:0007596 blood coagulation; GO:0005125 cytokine activity; GO:0005132 interferon-alpha/beta receptor binding; GO:0005615 extracellular space; GO:0006917 induction of apoptosis; GO:0006919 activation of caspase activity; GO:0008285 negative regulation of cell proliferation; GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter; GO:0030101 natural killer cell activation; GO:0032897 negative regulation of viral transcription; GO:0042100 B cell proliferation; GO:0045071 negative regulation of viral genome replication; GO:0045089 positive regulation of innate immune response; GO:0045343 regulation of MHC class I biosynthetic process; GO:0046597 negative regulation of virion penetration into host cell; GO:0051607 defense response to virus; GO:0060337 type I interferon-mediated signaling pathway; GO:0060338 regulation of type I interferon-mediated signaling pathway; GO:0071360 cellular response to exogenous dsRNA
Regulatory: For research purposes only

Applications of IFN Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin ¹
Western Blotting			5ug/ml

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP1435 detects a band of approximately 15kDa in A20 cell lysates.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Sheep anti Rabbit IgG:Biotin	2AB02B	1 ml	C E P WB
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	1 mg	E WB
Goat anti Rabbit IgG (Fc):FITC	STAR121F	1 mg	F
Goat anti Rabbit IgG (Fc):HRP	STAR121P	1 mg	E WB
Goat anti Rabbit IgG (H/L):HRP	STAR124P	1 mg	C E WB
Sheep anti Rabbit IgG:FITC	STAR34B	1 mg	C F
Sheep anti Rabbit IgG:RPE	STAR35A	1 ml	F
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:DyLight®549	STAR36D549GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®649	STAR36D649GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	0.1 mg	F IF WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Antigen Retrieval Buffer, pH8.0	BUF025A	500 ml	P
Antigen Retrieval Buffer, pH8.0	BUF025C	50 ml	P
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	0.5 ml	WB*

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