hnRNP H1 antibody

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Rabbit anti hnRNP H1

Rabbit anti human hnRNP H1 antibody recognizes heterogeneous nuclear ribonucleoprotein H1. This antibody has been extensively validated for western blotting using whole cell lysates.

hnRNP H1 Antibody with Control Lysate

Rabbit anti human hnRNP H1 antibody recognizes heterogeneous nuclear ribonucleoprotein H1. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00311 WB 100 µl
VPA00311KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human hnRNP H1 antibody recognizes heterogeneous nuclear ribonucleoprotein H1 (hnRNP H1).

Encoded by the HNRNPH1 gene, hnRNP H1 is a member of a subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hn RNPs). The hn RNPs are RNA binding proteins that complex with heterogeneous nuclear RNA. These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some may shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by the hnRNP H1 gene has three repeats of quasi-RRM domains that bind to RNA and is very similar to the family member HNRPF. HNRNPH1 may be associated with hereditary lymphedema type I. Alternatively spliced transcript variants have been described (provided by RefSeq, Mar 2012).

Rabbit anti Human hnRNP H1 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti hnRNP H1 detects a band of approximately 50 kDa in Raji cell lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 48-77 of human hnRNP H1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Raji lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P31943 Related reagents
Entrez Gene
HNRNPH1 Related reagents
GO Terms
GO:0000166 nucleotide binding
GO:0005515 protein binding
GO:0000398 nuclear mRNA splicing, via spliceosome
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0005681 spliceosomal complex
GO:0008266 poly(U) RNA binding
GO:0030530 heterogeneous nuclear ribonucleoprotein complex
GO:0015629 actin cytoskeleton
GO:0043484 regulation of RNA splicing
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of hnRNP H1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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