hnRNP H1 antibody

Rabbit anti hnRNP H1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
hnRNP H1

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VPA00311
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Rabbit anti Human hnRNP H1 antibody recognizes heterogeneous nuclear ribonucleoprotein H1 (hnRNP H1).

Encoded by the HNRNPH1 gene, hnRNP H1 is a member of a subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hn RNPs). The hn RNPs are RNA binding proteins that complex with heterogeneous nuclear RNA. These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some may shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by the hnRNP H1 gene has three repeats of quasi-RRM domains that bind to RNA and is very similar to the family member HNRPF. HNRNPH1 may be associated with hereditary lymphedema type I. Alternatively spliced transcript variants have been described (provided by RefSeq, Mar 2012).

Rabbit anti Human hnRNP H1 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti hnRNP H1 detects a band of approximately 50 kDa in Raji cell lysate.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 48-77 of human hnRNP H1
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P31943
Entrez Gene
HNRNPH1
GO Terms
GO:0000166 nucleotide binding
GO:0005515 protein binding
GO:0000398 nuclear mRNA splicing, via spliceosome
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0005681 spliceosomal complex
GO:0008266 poly(U) RNA binding
GO:0030530 heterogeneous nuclear ribonucleoprotein complex
GO:0015629 actin cytoskeleton
GO:0043484 regulation of RNA splicing
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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