Heat Shock Protein 90 Alpha antibody
Rabbit anti heat shock protein 90 alpha antibody recognizes heat shock protein 90 alpha also known as HSP90AA1 or LAP2. Heat shock protein 90 alpha is an isoform of the highly conserved heat shock protein 90. Heat shock protein 90 alpha expression is induced under conditions of cellular stress where upon it acts as a molecular chaperone.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to heat shock protein 90 alpha was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Recombinant human heat shock protein 90 alpha
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P07900
- Entrez Gene
- HSP90AA1
- GO Terms
- GO:0051082 unfolded protein binding
- GO:0005886 plasma membrane
- GO:0000086 G2/M transition of mitotic cell cycle
- GO:0006986 response to unfolded protein
- GO:0042026 protein refolding
- GO:0005829 cytosol
- GO:0007165 signal transduction
- GO:0005524 ATP binding
- GO:0007411 axon guidance
- GO:0030235 nitric-oxide synthase regulator activity
- GO:0030911 TPR domain binding
- GO:0034619 cellular chaperone-mediated protein complex assembly
- GO:0042470 melanosome
- GO:0042803 protein homodimerization activity
- GO:0045040 protein import into mitochondrial outer membrane
- GO:0045429 positive regulation of nitric oxide biosynthetic process
- GO:0046209 nitric oxide metabolic process
- GO:0050999 regulation of nitric-oxide synthase activity
- Regulatory
- For research purposes only
Applications of Heat Shock Protein 90 Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | 1/20 | 1/50 | |
| Immunohistology - Paraffin | 1/50 | 1/100 | |
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti heat shock protein 90 alpha antibody detects a band of approximately 100 kDa in cell lysates and tissue lysates under reducing conditions
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
|
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
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