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Heat Shock Protein 90 Alpha antibody

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Anti Heat Shock Protein 90 Alpha Antibody thumbnail image 1 Anti Heat Shock Protein 90 Alpha Antibody thumbnail image 2 Anti Heat Shock Protein 90 Alpha Antibody thumbnail image 3 Anti Heat Shock Protein 90 Alpha Antibody thumbnail image 4
Anti Heat Shock Protein 90 Alpha Antibody gallery image 1
Western blot analysis of cell and tissue lysates using Rabbit anti heat shock protein 90 alpha antibody
Anti Heat Shock Protein 90 Alpha Antibody gallery image 2
Immunohistochemistry of paraffin-embedded human prostate using Rabbit anti heat shock protein 90 alpha antibody at a dilution of 1/100
Anti Heat Shock Protein 90 Alpha Antibody gallery image 3
Immunohistochemistry of paraffin-embedded human lung using Rabbit anti heat shock protein 90 alpha antibody at a dilution of 1/100
Anti Heat Shock Protein 90 Alpha Antibody gallery image 4
Immunofluorescence analysis of U2OS cells using Rabbit anti heat shock protein 90 alpha antibody

Rabbit anti Heat Shock Protein 90 Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2473
Datasheet
IF P WB
SDS
50 µl loader

Rabbit anti heat shock protein 90 alpha antibody recognizes heat shock protein 90 alpha also known as HSP90AA1 or LAP2. Heat shock protein 90 alpha is an isoform of the highly conserved heat shock protein 90. Heat shock protein 90 alpha expression is induced under conditions of cellular stress where upon it acts as a molecular chaperone.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to heat shock protein 90 alpha was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Recombinant human heat shock protein 90 alpha

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P07900
Entrez Gene
HSP90AA1
GO Terms
GO:0051082 unfolded protein binding
GO:0005886 plasma membrane
GO:0000086 G2/M transition of mitotic cell cycle
GO:0006986 response to unfolded protein
GO:0042026 protein refolding
GO:0005829 cytosol
GO:0007165 signal transduction
GO:0005524 ATP binding
GO:0007411 axon guidance
GO:0030235 nitric-oxide synthase regulator activity
GO:0030911 TPR domain binding
GO:0034619 cellular chaperone-mediated protein complex assembly
GO:0042470 melanosome
GO:0042803 protein homodimerization activity
GO:0045040 protein import into mitochondrial outer membrane
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0046209 nitric oxide metabolic process
GO:0050999 regulation of nitric-oxide synthase activity
Regulatory
For research purposes only

Applications of Heat Shock Protein 90 Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/20 1/50
Immunohistology - Paraffin 1/50 1/100
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti heat shock protein 90 alpha antibody detects a band of approximately 100 kDa in cell lysates and tissue lysates under reducing conditions
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

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