Rabbit anti GSK3 Beta (pTyr216) antibody recognizes GSK3 beta when phosphorylated at tyrosine 216. GSK3 is a member of the CMGC family of kinases. GSK3 comprises two isoforms, alpha and beta, with 85% total homology and 98% homology between kinase domains. GSK3 beta is phosphorylated at tyrosine 216 via autophosphorylation, Fyn and PYK2. Phosphorylation of GSK3 beta at tyrosine 216 activates GSK3 alpha leading to phosphorylation of downstream targets such as the insulin receptor and glucose-6-phosphate.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to GSK3 BETA (pTyr216) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding tyrosine 216 of human GSK3 beta
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
RNA polymerase II transcription factor binding
epithelial to mesenchymal transition
positive regulation of cell-matrix adhesion
protein serine/threonine kinase activity
glycogen metabolic process
ER overload response
beta-catenin destruction complex
negative regulation of protein complex assembly
positive regulation of protein complex assembly
ubiquitin protein ligase binding
negative regulation of protein binding
positive regulation of Rac GTPase activity
protein kinase A catalytic subunit binding
negative regulation of apoptosis
positive regulation of protein catabolic process
positive regulation of protein export from nucleus
nerve growth factor receptor signaling pathway
tau-protein kinase activity
canonical Wnt receptor signaling pathway
superior temporal gyrus development
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of GSK3 Beta (pTyr216) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available