GSK3 Beta (pTyr216) antibody

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Rabbit anti GSK3 Beta (pTyr216)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2627 P WB 50 µl
Rabbit anti GSK3 Beta (pTyr216) antibody recognizes GSK3 beta when phosphorylated at tyrosine 216. GSK3 is a member of the CMGC family of kinases. GSK3 comprises two isoforms, alpha and beta, with 85% total homology and 98% homology between kinase domains. GSK3 beta is phosphorylated at tyrosine 216 via autophosphorylation, Fyn and PYK2. Phosphorylation of GSK3 beta at tyrosine 216 activates GSK3 alpha leading to phosphorylation of downstream targets such as the insulin receptor and glucose-6-phosphate.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to GSK3 BETA (pTyr216) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding tyrosine 216 of human GSK3 beta

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P49841 Related reagents
Entrez Gene
GSK3B Related reagents
GO Terms
GO:0001085 RNA polymerase II transcription factor binding
GO:0001837 epithelial to mesenchymal transition
GO:0001954 positive regulation of cell-matrix adhesion
GO:0002039 p53 binding
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005977 glycogen metabolic process
GO:0006983 ER overload response
GO:0007411 axon guidance
GO:0008013 beta-catenin binding
GO:0018105 peptidyl-serine phosphorylation
GO:0048015 phosphatidylinositol-mediated signaling
GO:0021766 hippocampus development
GO:0030877 beta-catenin destruction complex
GO:0031333 negative regulation of protein complex assembly
GO:0031334 positive regulation of protein complex assembly
GO:0031625 ubiquitin protein ligase binding
GO:0032091 negative regulation of protein binding
GO:0032855 positive regulation of Rac GTPase activity
GO:0034236 protein kinase A catalytic subunit binding
GO:0034747 Axin-APC-beta-catenin-GSK3B complex
GO:0043066 negative regulation of apoptosis
GO:0045732 positive regulation of protein catabolic process
GO:0046827 positive regulation of protein export from nucleus
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050321 tau-protein kinase activity
GO:0051059 NF-kappaB binding
GO:0060070 canonical Wnt receptor signaling pathway
GO:0071109 superior temporal gyrus development
Regulatory
For research purposes only

Applications of GSK3 Beta (pTyr216) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/50 1/100
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*