GSK3 Alpha antibody

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KO Validated

Anti GSK3 Alpha Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1

Anti GSK3 Alpha Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2

Anti GSK3 Alpha Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3

Anti GSK3 Alpha Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with GSK3 alpha antibody followed by detection with Goat anti Rabbit HRP conjugated(1/10,000,STAR208P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to GSK3 alpha (molecular weight 51 kDa)

Anti GSK3 Alpha Antibody (PrecisionAb Polyclonal Antibody) gallery image 2 — Western blot analysis of GSK3 alpha CRISPR knockout HEK293 (GSK3 Alpha KO) and wild type HEK293 (WT) whole cell lysates probed with (A) Rabbit anti GSK3 alpha antibody (**VPA00205**) followed by detection with HRP conjugated Goat anti Rabbit IgG (H/L) (1/10,000 STAR208P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 19 second and (B) 30 second exposure.

Anti GSK3 Alpha Antibody (PrecisionAb Polyclonal Antibody) gallery image 3 — Normalized signal intensity of GSK3 alpha from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

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Rabbit anti GSK3 Alpha

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: GSK3 Alpha

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Product Code Applications Pack Size List Price Your Price Qty

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VPA00205 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human GSK3 alpha antibody recognizes glycogen synthase kinase-3 (GSK3) alpha, also known as serine/threonine-protein kinase GSK3A.

Encoded by the GSK3A gene, GSK3 alpha encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease (provided by RefSeq, Oct 2011).

Rabbit anti Human GSK3 alpha antibody detects a band of 51 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti GSK3 alpha detects a band of approximately 51 kDa in HEK293 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Rabbit Ig fraction prepared by ammonium sulphate precipitation.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: KLH conjugated synthetic peptide between 76-105 amino acids from the N-terminal region of human GSK3 alpha
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

Synonyms: GSK3A
UniProt: P49840
Entrez Gene: GSK3A
GO Terms: GO:0005829 cytosol; GO:0004674 protein serine/threonine kinase activity; GO:0005524 ATP binding; GO:0006468 protein phosphorylation; GO:0008286 insulin receptor signaling pathway; GO:0010905 negative regulation of UDP-glucose catabolic process; GO:0048015 phosphatidylinositol-mediated signaling; GO:0030877 beta-catenin destruction complex; GO:0034236 protein kinase A catalytic subunit binding; View More GO Terms; GO:0045732 positive regulation of protein catabolic process; GO:0046325 negative regulation of glucose import; GO:0046627 negative regulation of insulin receptor signaling pathway; GO:0048011 nerve growth factor receptor signaling pathway; GO:0050321 tau-protein kinase activity; GO:0051348 negative regulation of transferase activity