GLI3 antibody

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Rabbit anti GLI3

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1456 E P* WB 50 µg
Rabbit anti Human GLI3 antibody recognizes the Human Zinc Finger protein 3 (GLI-3), also known as GLI-Kruppel family member 3, a 169.8 kDa protein belonging to the GLI C2H2-type zinc finger protein of transcription factors.

GLI-3 and the related GLI-2 proteins are the primary transcription factors that mediate Sonic hedgehog (Shh) signals in the mouse, GLI-2 being primarily a transcriptional activator while GLI-3 is a transcriptional repressor. GLI-3 plays a role in the development of the limbs and brain. It is expressed in a wide range of adult tissues including the colon, lung, spleen, testis and placenta.

Defects in GLI3 are causative of a range of disease states including Pallister-Hall syndrome, type IV preaxial polydactyly, acrocallosal syndrome (ACS) and Greig cephalo-poly-syndactyly syndrome. GLI proteins are also implicated as potent oncogenes in the embryonal carcinoma cell.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chicken
Rat
Amphibia
Dog
Monkey
Mouse
Chimpanzee
Quail
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human GLI3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to amino acid sequence 41-57 of human Gli3 protein.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P10071 Related reagents
Entrez Gene
GLI3 Related reagents
GO Terms
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005829 cytosol
GO:0005730 nucleolus
GO:0005929 cilium
GO:0008013 beta-catenin binding
GO:0008270 zinc ion binding
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0016564 transcription repressor activity
GO:0035035 histone acetyltransferase binding
GO:0042826 histone deacetylase binding
GO:0045060 negative thymic T cell selection
GO:0045879 negative regulation of smoothened signaling pathway
GO:0046638 positive regulation of alpha-beta T cell differentiation
GO:0046639 negative regulation of alpha-beta T cell differentiation
GO:0070242 thymocyte apoptosis
GO:0090090 negative regulation of canonical Wnt receptor signaling pathway
Regulatory
For research purposes only

Applications of GLI3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/6000
Immunohistology - Paraffin 1 1.25ug/ml
Western Blotting 1/500
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human brain, glioblastoma

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*
Histar Detection System STAR3000B 150 Tests C P