GLI3 antibody
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P ResetRabbit anti GLI3
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- GLI3
Antibody Quality and Performance Guarantee
| Rabbit anti Human GLI3 antibody recognizes the Human Zinc Finger protein 3 (GLI-3), also known as GLI-Kruppel family member 3, a 169.8 kDa protein belonging to the GLI C2H2-type zinc finger protein of transcription factors. GLI-3 and the related GLI-2 proteins are the primary transcription factors that mediate Sonic hedgehog (Shh) signals in the mouse, GLI-2 being primarily a transcriptional activator while GLI-3 is a transcriptional repressor. GLI-3 plays a role in the development of the limbs and brain. It is expressed in a wide range of adult tissues including the colon, lung, spleen, testis and placenta. Defects in GLI3 are causative of a range of disease states including Pallister-Hall syndrome, type IV preaxial polydactyly, acrocallosal syndrome (ACS) and Greig cephalo-poly-syndactyly syndrome. GLI proteins are also implicated as potent oncogenes in the embryonal carcinoma cell. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Chicken Rat Amphibia Expected from Sequence Dog Monkey Mouse Chimpanzee Quail - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human GLI3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding to amino acid sequence 41-57 of human Gli3 protein.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/6000 | |
| Immunohistology - Paraffin 1 | |
1.25ug/ml | |
| Western Blotting | |
1/500 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human brain, glioblastoma
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| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | Log in | |||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
- RRID
- AB_2111920
- UniProt
- P10071
- Entrez Gene
- GLI3
- GO Terms
- GO:0000122 negative regulation of transcription from RNA polymerase II promoter
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0005829 cytosol
- GO:0005730 nucleolus
- GO:0005929 cilium
- GO:0008013 beta-catenin binding
- GO:0008270 zinc ion binding
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0016564 transcription repressor activity
- View More GO Terms
- GO:0035035 histone acetyltransferase binding
- GO:0042826 histone deacetylase binding
- GO:0045060 negative thymic T cell selection
- GO:0045879 negative regulation of smoothened signaling pathway
- GO:0046638 positive regulation of alpha-beta T cell differentiation
- GO:0046639 negative regulation of alpha-beta T cell differentiation
- GO:0070242 thymocyte apoptosis
- GO:0090090 negative regulation of canonical Wnt receptor signaling pathway
AHP1456
130715
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