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GATA3 antibody

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Anti GATA3 Antibody thumbnail image 1 Anti GATA3 Antibody thumbnail image 2 Anti GATA3 Antibody thumbnail image 3
Anti GATA3 Antibody gallery image 1
Western blot analysis of GATA3 was performed by loading 15 μg of SH-SY5Y whole cell lysate (WCL), SH-SY5Y nuclear extract (NE) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked for at least 1 hour. The membrane was probed with GATA3 polyclonal antibody (AHP2403) at a dilution of 1/1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with an HRP-conjugated goat anti-rabbit IgG secondary antibody for 1 hour. Chemiluminescence was performed for band detection.
Anti GATA3 Antibody gallery image 2
Immunohistochemistry analysis of GATA3 showing staining in the nucleus of paraffin-embedded human tonsil tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with GATA3 polyclonal antibody (AHP2403) diluted in 3% BSA-PBS at a dilution of 1/200 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Anti GATA3 Antibody gallery image 3
Immunofluorescent analysis of GATA3 (green) in MCF7 (left panel) and SH-SY5Y cells (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with GATA3 polyclonal antibody (AHP2403) at a dilution of 1/50 for at least 1 hour at room temperature, washed with PBS, and incubated with a goat anti-rabbit IgG secondary antibody (green) for 30 minutes at room temperature. Cells were counterstained with phalloidin (F-actin, red) and Hoechst 33342 (Nucleus, blue). Cells were viewed at 20x magnification.

Rabbit anti GATA3

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
GATA3

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Product Code Applications Pack Size List Price Your Price Qty
AHP2403
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IC IF P * WB 50 µg loader
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loader

Rabbit anti GATA3 antibody recognizes GATA3, also known as Trans-acting T-cell-specific transcription factor GATA-3 or GATA-binding factor 3. The protein is a member of the GATA family of transcription factors, which has received its name from binding DNA sequences containing the GATA consensus motif 5'-[AT]GATA[AG]-3'  (uniprot P15976).

GATA3 has been shown to play a critical role in T cell development and the differentiation of T helper 2 (Th2) cells (Ho et al.1991). In addition, GATA3 has been implied to have tissue specific functions such as a regulatory role in the development of the mammary gland epithelium and skin tissue (Chou et al. 2010).

Aberrant GATA3 levels have been identified in a variety of cancers including breast cancer. Indeed, GATA3 has become a marker for certain breast cancer types and GATA3 antibodies are commonly used for the immunohistochemical evaluation of breast cancer samples (Krings et al. 2014).

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to GATA3 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein G.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
1 mg/ml Bovine Serum Albumin
30% Glycerol
Immunogen
Purified partial recombinant GATA3 protein expressed in E. coli
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunocytochemistry 1/50 1/500
Immunofluorescence 1/50 1/500
Immunohistology - Paraffin 1 1/50 1/500
Western Blotting 1/1000
  1. 1This antibody requires heat-mediated antigen retrieval prior to staining. Bio-Rad recommend citrate buffer pH6.0 for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti GATA3 antibody recognizes a band of approximately 55 kDa in SH-SY5Y cell lysate and SH-SY5Y nuclear extract

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Synonyms
GATA-3
GATA-Binding Factor 3
UniProt
P23771
Entrez Gene
GATA3
GO Terms
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0006952 defense response
GO:0007596 blood coagulation
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0006355 regulation of transcription, DNA-dependent
GO:0006366 transcription from RNA polymerase II promoter
GO:0009653 anatomical structure morphogenesis
GO:0008134 transcription factor binding
GO:0008270 zinc ion binding
GO:0043565 sequence-specific DNA binding
GO:0043627 response to estrogen stimulus
GO:0071837 HMG box domain binding

AHP2403

160223 161159

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