GAPDH antibody

100% Secure


Rabbit anti GAPDH (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP996 WB 0.1 mg
Rabbit anti GAPDH (C-Terminal) antibody recognizes an epitope within the C-Terminal region (CT) of Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a glycolytic enzyme which plays a key role in energy production, and has also been implicated in numerous cellular processes.

GAPDH is a homotetramer molecule consisting of four 36kDa subunits, constitutively expressed in most cells and tissues, and is responsible for the generation of energy during carbohydrate metabolism, catalysing the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate.

The role of GAPDH as a multifunctional protein has emerged from many studies, confirming its involvement in critical nuclear pathways, apoptosis, membrane transport and fusion, DNA replication and repair and phosphotransferase activity. Furthermore, the ability of GAPDH to bind with high affinity to ß-amyloid precursor protein (Alzheimers disease) and selectively with CAG mutated proteins, including huntingtin (Huntingdons disease) and the androgen receptor (spinobulbar muscular atrophy), has focused many studies towards the role of GAPDH in the pathogenesis of neurodegenerative diseases (De Santo et al. 2010).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Antisera to human GAPDH (CT) were raised by repeated immunisation of rabbits with highly purified antigen.

Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A 16 amino acid peptide located near human GAPDH carboxy-terminus.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P04406 Related reagents
Entrez Gene
GAPDH Related reagents
GO Terms
GO:0005515 protein binding
GO:0016020 membrane
GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity
GO:0005829 cytosol
GO:0005634 nucleus
GO:0006094 gluconeogenesis
GO:0006096 glycolysis
GO:0055114 oxidation-reduction process
GO:0050821 protein stabilization
GO:0035605 peptidyl-cysteine S-nitrosylase activity
GO:0035606 peptidyl-cysteine S-trans-nitrosylation
GO:0048471 perinuclear region of cytoplasm
GO:0051287 NAD binding
GO:0051402 neuron apoptosis
Regulatory
For research purposes only

Applications of GAPDH antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.5 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP996 detects a band of approximately 37kDa in HeLa cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Application Based External Images

Western Blotting

Product Specific References

References for GAPDH antibody

  1. De Santo, C. et al. (2010) Invariant NKT cells modulate the suppressive activity of IL-10-secreting neutrophils differentiated with serum amyloid A.
    Nat Immunol. 11 (11): 1039-46.
  2. Weeke-Klimp, A. et al. (2010) Epicardium-derived cells enhance proliferation, cellular maturation and alignment of cardiomyocytes.
    J Mol Cell Cardiol. 49: 606-16.
  3. Deacon, K. and Knox, A.J. (2010) Endothelin-1 (ET-1) increases the expression of remodeling genes in vascular smooth muscle through linked calcium and cAMP pathways: role of a phospholipase A(2)(cPLA(2))/cyclooxygenase-2 (COX-2)/prostacyclin receptor-dependent autocrine loop.
    J Biol Chem. 285: 25913-27.

Further Reading

  1. Mazzola, J.L. & Sirover, M.A. (2001) Reduction of glyceraldehyde-3-phosphate dehydrogenase activity in Alzheimer's disease and in Huntington's disease fibroblasts.
    J Neurochem. 76 (2): 442-9.
  2. Sirover, M.A. (2005) New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells.
    J Cell Biochem. 95 (1): 45-52.