Galectin-3 antibody

Rabbit anti Human Galectin-3:Biotin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Format
Purified
Specificity
Galectin-3

Product Code Applications Pack Size List Price Your Price Qty
AHP1481B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 50 µg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rabbit anti Human Galectin-3 antibody recognizes human Galectin-3 (Gal-3), also known as the Mac-2 antigen. Gal-3 is a 250 amino acid ~35 kDa member of the galectin family of beta-galactoside-binding proteins, which is involved in both intracellular and extracellular functions and exhibits anti-apoptotic properties.

Gal-3 is implicated in many biological processes, including cell differentiation, growth, adhesion, angiogenesis and inflammation, and is expressed in a wide range of neoplasms. Gal-3 acts as a regulator of tumor metastasis expression correlates with tumor progression in liver, thyroid, colon, gastric and breast carcinomas (Califice et al. 2004).
PrecisionAb Antibodies

Target Species
Human
Western Blotting
AHP1481B may be used under either reducing or non-reducing conditions with PHP188 as the positive control.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Biotin - lyophilized
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antiserum to human galectin-3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Western Blotting 0.1 0.2ug/ml

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Recombinant Human Galectin-3 PHP188 E FN WB 50 µg loader
List Price Your Price
loader
Description Recombinant Human Galectin-3

References for Galectin-3 antibody

  1. Chen, Y.X. et al. (2015) The Sirt1 activator SRT1720 attenuates angiotensin II-induced atherosclerosis in apoE⁻/⁻ mice through inhibiting vascular inflammatory response.
    Biochem Biophys Res Commun. 465 (4): 732-8.

Further Reading

  1. Dumic, J. et al. (2006) Galectin-3: an open-ended story.
    Biochim Biophys Acta. 1760 (4): 616-35.

Synonyms
MAC-2
RRID
AB_1102581
UniProt
P17931
Entrez Gene
LGALS3
GO Terms
GO:0005886 plasma membrane
GO:0005529 sugar binding
GO:0005634 nucleus
GO:0005743 mitochondrial inner membrane
GO:0019863 IgE binding
GO:0030154 cell differentiation
GO:0045087 innate immune response
View more products with GALECTIN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products