Galectin-1 antibody

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Rabbit anti Galectin-1

Galectin-1 antibody recognizes galectin-1, also known as Gal-1, 14 kDa laminin-binding protein, HLBP14, 14 kDa lectin, beta-galactoside-binding lectin L-14-I, galaptin, HBL, HPL, or S-Lac lectin 1. The antibody has been extensively validated for western blotting using whole cell lysates.

Galectin-1 Antibody with Control Lysate

Galectin-1 antibody recognizes galectin-1, also known as Gal-1, 14 kDa laminin-binding protein, HLBP14, 14 kDa lectin, beta-galactoside-binding lectin L-14-I, galaptin, HBL, HPL, lactose-binding lectin 1, Lectin galactoside-binding soluble 1, putative MAPK-activating protein PM12 or S-Lac lectin 1.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00080 WB 100 µl
VPA00080KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human galectin-1 antibody recognizes human galectin-1 (gal-1) also known lactose-binding lectin 1. Gal-1 is a member of the galectin family of beta-galactoside-binding proteins, which exhibits both intracellular and extracellular functions and is differentially expressed by a wide range of normal and neoplastic tissues.

Gal-1 has been implicated in several major cellular functions including proliferation, motility, hematopoietic cell differentiation, apoptosis promotion and immunoregulatory effects. The expression of gal-1 by neuronal and glial cells correlates with neuroregeneration following injury, and studies have shown the importance of gal-1 to primary sensory neurons and synaptic connections in the spinal cord, during embryonic development.

Rabbit anti Human galectin-1 antibody detects gal-1 as a single band of 13 kDa in a range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E.coli derived recombinant human galectin-1.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P09382 Related reagents
Entrez Gene
LGALS1 Related reagents
GO Terms
GO:0016936 galactoside binding
GO:0006915 apoptosis
GO:0004871 signal transducer activity
GO:0005578 proteinaceous extracellular matrix
GO:0005615 extracellular space
GO:0005737 cytoplasm
GO:0042981 regulation of apoptosis
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Galectin-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Rabbit anti galectin-1 detects a band of approximately 13 kDa in K562 cell lysates.
Western Blotting
Rabbit anti galectin-1 detects a band of approximately 13 kDa in K562 cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB