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Flotillin-1 antibody

Goat anti Flotillin-1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
Flotillin-1

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VPA00126
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Goat anti Human flotillin-1 antibody recognizes the flotillin-1 protein

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. Flotillin-1, encloded by the FLOT1 gene, is a caveolae-associated, integral membrane protein. The function of flotillin-1 has not been determined (provided by RefSeq, Jul 2008).

Goat anti Human flotillin-1 antibody detects a band of 47 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti flotillin-1 detects a band of approximately 47 kDa in K562 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution
TRIS buffered saline.
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Immunogen
Peptide with the sequence C-SISQVNHKPLRTA, from the C Terminus of the protein sequence.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

UniProt
O75955
Entrez Gene
FLOT1
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0005624 membrane fraction
GO:0005768 endosome
GO:0034451 centriolar satellite
GO:0042470 melanosome

VPA00126

161214 167737

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