Flotillin-1 antibody
Goat anti Flotillin-1
Flotillin 1 antibody recognizes Flotillin-1. The antibody has been extensively validated for western blotting using whole cell lysates.
Flotillin-1 Antibody with Control Lysate
Flotillin 1 antibody recognizes Flotillin-1. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00126 | WB | 100 µl | ||||
| VPA00126KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Goat anti Human flotillin-1 antibody recognizes the flotillin-1 protein
Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. Flotillin-1, encloded by the FLOT1 gene, is a caveolae-associated, integral membrane protein. The function of flotillin-1 has not been determined (provided by RefSeq, Jul 2008).
Goat anti Human flotillin-1 antibody detects a band of 47 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. Flotillin-1, encloded by the FLOT1 gene, is a caveolae-associated, integral membrane protein. The function of flotillin-1 has not been determined (provided by RefSeq, Jul 2008).
Goat anti Human flotillin-1 antibody detects a band of 47 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti flotillin-1 detects a band of approximately 47 kDa in K562 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Goat polyclonal antibody purified by affinity chromatography.
- Preparation
- 20μl Goat polyclonal antibody purified by affinity chromatography
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3) 0.5 % BSA
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3) 0.5 % BSA
- Immunogen
- Peptide with the sequence C-SISQVNHKPLRTA, from the C Terminus of the protein sequence.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg K562 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- O75955 Related reagents
- Entrez Gene
- FLOT1 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0005624 membrane fraction
- GO:0005768 endosome
- GO:0034451 centriolar satellite
- GO:0042470 melanosome
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Flotillin-1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB | ||
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB |
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