Fibronectin antibody
Sheep anti fibronectin antibody (VPA00045) used to evaluate fibronectin expression in murine renal tissue by immunofluorescence.
Image caption:
SH045 impact on renal fibrogenesis after UUO.(C) Representative images of control and UUO-injured kidneys stained with fibronectin (magnification: 400×). Scale bars are 50 μm. (D) Quantification in fibronectin+ area (control n = 6, UUO n = 8). Data expressed as means ± SD. Two-way ANOVA followed by Sidak’s multiple comparisons post hoc test. * p <0.05, ** p <0.01, and **** p <0.0001 defined as significant. ns, not statistically significant.
From: Zheng Z, Xu Y, Krügel U, Schaefer M, Grune T, Nürnberg B, Köhler MB, Gollasch M, Tsvetkov D, Markó L.
In Vivo Inhibition of TRPC6 by SH045 Attenuates Renal Fibrosis in a New Zealand Obese (NZO) Mouse Model of Metabolic Syndrome.
Int J Mol Sci. 2022 Jun 20;23(12):6870.
doi: 10.3390/ijms23126870..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Sheep anti Fibronectin
- Product Type
- PrecisionAb Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
- Specificity
- Fibronectin
Antibody Quality and Performance Guarantee
| Sheep anti Human fibronectin antibody detects human fibronectin, a multi-domain protein involved in the connection of cells to the extracellular matrix. Fibronectin is mostly found in dimeric form, consisting of two nearly identical polypeptide chains linked by a pair of C-terminal disulfide bonds. Each fibronectin monomer has a molecular weight of 230–250 kDa. Fibronectin can bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectin is present in the body in two forms; plasma fibronectin (soluble dimeric form) is secreted by hepatocytes, whilst cellular fibronectin (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing and blood coagulation (Schwarzbauer and DiSimone, 2011) as well as pathologies such as metastasis (Van Obberghen-Schilling et al. 2011). Sheep anti Human fibronectin antibody recognizes fibronectin as a single band of approximately 260 kDa in a range of human cell lysates by western blotting under reducing conditions. Cross reactivity with rodent cell line lysates is also observed. |
|
- Target Species
- Human
- Western Blotting
- Sheep anti Fibronectin detects a band of approxiamtely 260 kDa in A549 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Sheep polyclonal antibody purified by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3).
- Immunogen
- Highly pure human fibronectin.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
References for Fibronectin antibody
-
Chen, R. et al. (2018) The optimization and production of stable homogeneous amine enriched surfaces with characterized nanotopographical properties for enhanced osteoinduction of mesenchymal stem cells.
J Biomed Mater Res A. 106 (7): 1862-77. -
Zheng, Z. et al. (2022) In Vivo Inhibition of TRPC6 by SH045 Attenuates Renal Fibrosis in a New Zealand Obese (NZO) Mouse Model of Metabolic Syndrome.
Int J Mol Sci. 23(12):6870.
VPA00045
161360
211014
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