Fibronectin antibody

Sheep anti Fibronectin

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
Fibronectin

Product Code Applications Pack Size List Price Your Price Qty
VPA00045
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl
List Price Your Price
Search for Batch Specific Datasheets

Sheep anti Human fibronectin antibody detects human fibronectin, a multi-domain protein involved in the connection of cells to the extracellular matrix. Fibronectin is mostly found in dimeric form, consisting of two nearly identical polypeptide chains linked by a pair of C-terminal disulfide bonds. Each fibronectin monomer has a molecular weight of 230–250 kDa. Fibronectin can bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectin is present in the body in two forms; plasma fibronectin (soluble dimeric form) is secreted by hepatocytes, whilst cellular fibronectin (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.

Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing and blood coagulation (Schwarzbauer and DiSimone, 2011) as well as pathologies such as metastasis (Van Obberghen-Schilling et al. 2011).

Sheep anti Human fibronectin antibody recognizes fibronectin as a single band of approximately 260 kDa in a range of human cell lysates by western blotting under reducing conditions. Cross reactivity with rodent cell line lysates is also observed.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Sheep anti Fibronectin detects a band of approxiamtely 260 kDa in A549 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Sheep polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Highly pure human fibronectin.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader
List Price Your Price
loader
Description Donkey anti Sheep/Goat IgG:HRP

References for Fibronectin antibody

  1. Chen, R. et al. (2018) The optimization and production of stable homogeneous amine enriched surfaces with characterized nanotopographical properties for enhanced osteoinduction of mesenchymal stem cells
    Journal of Biomedical Materials Research Part A. 106 (7): 1862-77.
  2. Zheng, Z. et al. (2022) In Vivo Inhibition of TRPC6 by SH045 Attenuates Renal Fibrosis in a New Zealand Obese (NZO) Mouse Model of Metabolic Syndrome.
    Int J Mol Sci. 23 (12)Jun 20 [Epub ahead of print].

UniProt
P02751
Entrez Gene
FN1
View more products with FIBRONECTIN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products