Fibronectin antibody
Sheep anti Human fibronectin antibody detects human fibronectin, a multi-domain protein involved in the connection of cells to the extracellular matrix. Fibronectin is mostly found in dimeric form, consisting of two nearly identical polypeptide chains linked by a pair of C-terminal disulfide bonds. Each fibronectin monomer has a molecular weight of 230–250 kDa. Fibronectin can bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectin is present in the body in two forms; plasma fibronectin (soluble dimeric form) is secreted by hepatocytes, whilst cellular fibronectin (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.
Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing and blood coagulation (Schwarzbauer and DiSimone, 2011) as well as pathologies such as metastasis (Van Obberghen-Schilling et al. 2011).
Sheep anti Human fibronectin antibody recognizes fibronectin as a single band of approximately 260 kDa in a range of human cell lysates by western blotting under reducing conditions. Cross reactivity with rodent cell line lysates is also observed.
Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing and blood coagulation (Schwarzbauer and DiSimone, 2011) as well as pathologies such as metastasis (Van Obberghen-Schilling et al. 2011).
Sheep anti Human fibronectin antibody recognizes fibronectin as a single band of approximately 260 kDa in a range of human cell lysates by western blotting under reducing conditions. Cross reactivity with rodent cell line lysates is also observed.
Product Details
- Target Species
- Human
- Western Blotting
- Sheep anti Fibronectin detects a band of approxiamtely 260 kDa in A549 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Sheep polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Highly pure human fibronectin.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P02751
- Entrez Gene
- FN1
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Fibronectin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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