FGF Basic

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  • Recombinant Human FGF Basic
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP105E, FN, WBdatasheet pdfdatasheet pdf50 µg
    PHP105
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Recombinant Human FGF basic represents the C-terminal protion of human fibroblast growth factor 2 (A135 - S288).

      Fibroblast growth factor basic (FGF basic), also known as FGF 2, is a heparin binding growth factor which has stimulatory activity on a range of cells of mesenchymal, neuroectodermal and endothelial origin.
      Note: FGF basic is sensitive to acidic conditions.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified recombinant protein expressed in E. coli - lyophilised
    • Reconstitution
      Reconstitute with 0.5ml Tris (5mM, pH7.6).
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. Further dilutions may be prepared in a buffer containing a carrier protein (eg 0.1% BSA).
    • Preparation
    • Preservative Stabilisers
      None present
    • Activity
      2 x 106 units/mg
    • Purity
      >95% by SDS PAGE and HPLC analysis
    • Approx. Protein Concentrations
      Total protein concentration 0.1 mg/ml after reconstitution.
    • Protein Molecular Weight
      17.2kD (154 amino acid sequence)
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <0.1ng/ug
    • Test Principle
    • Buffer Solution
      TRIS buffered saline.
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4 ng/well
      Functional Assays0.110 ng/ml
      Western Blotting1.53.0 ng/lane

    • Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      This product may be used as a standard for ELISA applications with either AHP1038 or AHP1038B.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      This product may be used as the positive control for Wester Blot applications with either AHP1038 or AHP1038B.
    • Instructions For Use

    Additional FGF Basic Formats

    Formats Applications Sizes available
    FGF Basic : Rec. Protein E, FN, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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