FADD antibody

Rabbit anti FADD

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
FADD

Product Code Applications Pack Size List Price Your Price Qty
AHP2465
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SDS Safety Datasheet SDS
P WB 50 µl loader
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Rabbit anti FADD antibody recognizes FADD, also known as Fas-associated death domain protein, MORT1, growth-inhibiting gene 3 protein or mediator of receptor induced toxicity. FADD is a 23 kDa protein consisting of a C-terminal death domain and an N-terminal death effector domain. FADD is involved in forming the death-inducing signalling complex (DISC) during apoptosis.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to FADD was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Recombinant human FADD
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/50 1/200
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti FADD antibody detects a band of approximately 25 kDa in cell lysates and tissue lysates under reducing conditions

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UniProt
Q13158
Entrez Gene
FADD
GO Terms
GO:0005829 cytosol
GO:0007165 signal transduction
GO:0005123 death receptor binding
GO:0006919 activation of caspase activity
GO:0008625 induction of apoptosis via death domain receptors
GO:0008633 activation of pro-apoptotic gene products
GO:0042802 identical protein binding
GO:0031264 death-inducing signaling complex
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0044419 interspecies interaction between organisms
GO:0070265 necrotic cell death
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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