Endonuclease G antibody
Rabbit anti endonuclease G antibody (AHP730) used for the evaluation of Endo G expression in rat mitochondria by western blotting.
Image caption:
Release of mitochondrial intermembrane space proteins.
Mitochondria were incubated as described in the legend of fig. 1, whereupon they were sedimented by centrifugation and proteins of the resulting pellets (P) and supernatants (S) were analyzed by immunoblotting. Panel A: release of cytochrome c, Smac/DIABLO, Omi/HtrA2, AIF, endonuclease G, and LACTB by histones, tBID and p53. The concentrations used were: 5 μM histone H1.2; 10 μM histone H2A, 10 μM histone H2B, 5 μM histone H3, 5 μM histone H4, 1 μM tBID, and 1 μM p53. Panel B: effect of cyclosporin A on the histone-induced release of cytochrome c and Smac/DIABLO. Panel C: accessibility of AIF and LACTB to trypsin hydrolysis in the presence of histones.
From: Cascone A, Bruelle C, Lindholm D, Bernardi P, Eriksson O (2012)
Destabilization of the Outer and Inner Mitochondrial Membranes by Core and Linker Histones.
PLoS ONE 7(4): e35357.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti endonuclease G antibody (AHP730) used for the evaluation of EndoG expression in HCC1187 cells treated with TG02 in vitro by western blotting.
Image caption:
TG02 triggers apoptosis through caspase-dependent and independent pathways
(A) HCC1187 cells were treated with TG02 (1 μM) for different times (0, 6, 12 and 24 hours). Loss of mitochondrial membrane potential and viability were analyzed by flow cytometry after staining with TMRE or propidium iodide (PI), respectively. (B) Expression of proteins involved in apoptotic mechanisms was analyzed by Western blot after treatment of HCC1187 cells with TG02 (1 μM) at the indicated times. GAPDH was used as loading control. (C) Release of proteins from the intermembrane space of mitochondria in HCC1187 cells treated for 12 and 24 hours with TG02 (1 μM) was analyzed by Western blot after subcellular fractionation. COX IV and ERK1/2 were analyzed as mitochondrial and cytosolic markers, respectively. (D) Role of caspases in TG02-induced cell death. Induction of cell death by TG02 was analyzed in HCC1187 cells by flow cytometry after 1 hour of pretreatment with the pan-caspase inhibitor Z-VAD-FMK (25 μM), followed by treatment with TG02 (1 μM) for 24 hours.
From: Ortiz-Ruiz MJ, Álvarez-Fernández S, Parrott T, Zaknoen S, Burrows FJ, Ocaña A, Pandiella A, Esparís-Ogando A.
Therapeutic potential of ERK5 targeting in triple negative breast cancer.
Oncotarget. 2014 Nov 30;5(22):11308-18.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Endonuclease G
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Endonuclease G
Antibody Quality and Performance Guarantee
| Rabbit anti Endonuclease G antibody recognizes human endonuclease G, also known as endoG. Endo G is a 297 amino acid, including a 48 amino acid transit peptide ~36 kDa mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. EndoG is reported to act independently of caspases and may represent a caspase independent apoptotic pathway initiated from the mitochondria (Li et al. 2001). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Expected from Sequence Mouse Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to endonuclease G were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide (NaN3) - Immunogen
- Synthetic peptide GGPRGPGELAKYGLP corresponding to amino acids 55 - 70 of endonuclease G.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 |  |
15ug/ml | |
| Western Blotting | |
1ug/ml | 2ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human pancreas
- Western Blotting
- AHP730 detects a band of approximately 35kDa in HepG2 and 3T3 cell lysates.
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References for Endonuclease G antibody
-
Maiso, P. et al. (2008) The insulin-like growth factor-I receptor inhibitor NVP-AEW541 provokes cell cycle arrest and apoptosis in multiple myeloma cells.
Br J Haematol. 141 (4): 470-82. -
Ocio, E.M. et al. (2009) Zalypsis: a novel marine-derived compound with potent antimyeloma activity that reveals high sensitivity of malignant plasma cells to DNA double-strand breaks.
Blood. 113 (16): 3781-91. -
Colado, E. et al. (2011) Zalypsis has in vitro activity in acute myeloid blasts and leukemic progenitor cells through the induction of a DNA damage response.
Haematologica. 96: 687-95. -
Cascone, A. et al. (2012) Destabilization of the outer and inner mitochondrial membranes by core and linker histones.
PLoS One. 7 (4): e35357. -
Álvarez-Fernández S et al. (2013) Potent antimyeloma activity of a novel ERK5/CDK inhibitor.
Clin Cancer Res. 19 (10): 2677-87. -
Ortiz-Ruiz, M.J. et al. (2014) Therapeutic potential of ERK5 targeting in triple negative breast cancer.
Oncotarget. 5 (22): 11308-18. -
Amoury, M. et al. (2016) A novel approach for targeted elimination of CSPG4-positive triple-negative breast cancer cells using a MAP tau-based fusion protein.
Int J Cancer. 139 (4): 916-27.
Further Reading
-
Li, L.Y. et al. (2001) Endonuclease G is an apoptotic DNase when released from mitochondria.
Nature. 412 (6842): 95-9.
- RRID
- AB_324300
- UniProt
- Q14249
- Entrez Gene
- ENDOG
- GO Terms
- GO:0003676 nucleic acid binding
- GO:0005739 mitochondrion
- GO:0004519 endonuclease activity
- GO:0046872 metal ion binding
AHP730
159969
160627
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