Rabbit anti Endonuclease G antibody
recognizes human endonuclease G, also known as endoG. Endo G is a 297 amino acid, including a 48 amino acid transit peptide ~36 kDa mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis.
EndoG is reported to act independently of caspases and may represent a caspase independent apoptotic pathway initiated from the mitochondria (Li et al. 2001
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to endonuclease G were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Synthetic peptide GGPRGPGELAKYGLP corresponding to amino acids 55 - 70 of endonuclease G.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
nucleic acid binding
metal ion binding
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Endonuclease G antibody
|Immunohistology - Paraffin 1
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human pancreas
- Western Blotting
- AHP730 detects a band of approximately 35kDa in HepG2 and 3T3 cell lysates.
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Secondary Antibodies Available
Useful Reagents Available
Application Based External Images
Product Specific References
References for Endonuclease G antibody
Colado, E. et al. (2011) Zalypsis has in vitro activity in acute myeloid blasts and leukemic progenitor cells through the induction of a DNA damage response.
Haematologica. 96: 687-95.
Amoury, M. et al. (2016) A novel approach for targeted elimination of CSPG4-positive triple-negative breast cancer cells using a MAP tau-based fusion protein.
Int J Cancer. 139 (4): 916-27.
Maiso, P. et al. (2008) The insulin-like growth factor-I receptor inhibitor NVP-AEW541 provokes cell cycle arrest and apoptosis in multiple myeloma cells.
Br J Haematol. 141 (4): 470-82.
Cascone, A. et al. (2012) Destabilization of the outer and inner mitochondrial membranes by core and linker histones.
PLoS One. 7 (4): e35357.
Álvarez-Fernández S et al. (2013) Potent antimyeloma activity of a novel ERK5/CDK inhibitor.
Clin Cancer Res. 19 (10): 2677-87.
Ortiz-Ruiz, M.J. et al. (2014) Therapeutic potential of ERK5 targeting in triple negative breast cancer.
Oncotarget. 5 (22): 11308-18.
Ocio, E.M. et al. (2009) Zalypsis: a novel marine-derived compound with potent antimyeloma activity that reveals high sensitivity of malignant plasma cells to DNA double-strand breaks.
Blood. 113 (16): 3781-91.
Li, L.Y. et al. (2001) Endonuclease G is an apoptotic DNase when released from mitochondria.
Nature. 412 (6842): 95-9.