Endonuclease G antibody




EndoG is reported to act independently of caspases and may represent a caspase independent apoptotic pathway initiated from the mitochondria (Li et al. 2001).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Expected from Sequence Mouse Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to endonuclease G were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide (NaN3) - Immunogen
- Synthetic peptide GGPRGPGELAKYGLP corresponding to amino acids 55 - 70 of endonuclease G.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q14249
- Entrez Gene
- ENDOG
- GO Terms
- GO:0003676 nucleic acid binding
- GO:0005739 mitochondrion
- GO:0004519 endonuclease activity
- GO:0046872 metal ion binding
- Regulatory
- For research purposes only
Applications of Endonuclease G antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunohistology - Paraffin 1 | 15ug/ml | ||
Western Blotting | 1ug/ml | 2ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Histology Positive Control Tissue
- Human pancreas
- Western Blotting
- AHP730 detects a band of approximately 35kDa in HepG2 and 3T3 cell lysates.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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|
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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|
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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|
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for Endonuclease G antibody
-
Colado, E. et al. (2011) Zalypsis has in vitro activity in acute myeloid blasts and leukemic progenitor cells through the induction of a DNA damage response.
Haematologica. 96: 687-95. -
Amoury, M. et al. (2016) A novel approach for targeted elimination of CSPG4-positive triple-negative breast cancer cells using a MAP tau-based fusion protein.
Int J Cancer. 139 (4): 916-27. -
Maiso, P. et al. (2008) The insulin-like growth factor-I receptor inhibitor NVP-AEW541 provokes cell cycle arrest and apoptosis in multiple myeloma cells.
Br J Haematol. 141 (4): 470-82. -
Cascone, A. et al. (2012) Destabilization of the outer and inner mitochondrial membranes by core and linker histones.
PLoS One. 7 (4): e35357. -
Álvarez-Fernández S et al. (2013) Potent antimyeloma activity of a novel ERK5/CDK inhibitor.
Clin Cancer Res. 19 (10): 2677-87. -
Ortiz-Ruiz, M.J. et al. (2014) Therapeutic potential of ERK5 targeting in triple negative breast cancer.
Oncotarget. 5 (22): 11308-18. -
Ocio, E.M. et al. (2009) Zalypsis: a novel marine-derived compound with potent antimyeloma activity that reveals high sensitivity of malignant plasma cells to DNA double-strand breaks.
Blood. 113 (16): 3781-91.
Further Reading
-
Li, L.Y. et al. (2001) Endonuclease G is an apoptotic DNase when released from mitochondria.
Nature. 412 (6842): 95-9.
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