Endonuclease G antibody

Rabbit anti Endonuclease G

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Endonuclease G

Product Code Applications Pack Size List Price Your Price Qty
AHP730
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
P * WB 0.1 mg loader
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Rabbit anti Endonuclease G antibody recognizes human endonuclease G, also known as endoG. Endo G is a 297 amino acid, including a 48 amino acid transit peptide ~36 kDa mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis.

EndoG is reported to act independently of caspases and may represent a caspase independent apoptotic pathway initiated from the mitochondria (Li et al. 2001).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat Expected from Sequence
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to endonuclease G were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
Immunogen
Synthetic peptide GGPRGPGELAKYGLP corresponding to amino acids 55 - 70 of endonuclease G.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 15ug/ml
Western Blotting 1ug/ml 2ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human pancreas
Western Blotting
AHP730 detects a band of approximately 35kDa in HepG2 and 3T3 cell lysates.

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References for Endonuclease G antibody

  1. Colado, E. et al. (2011) Zalypsis has in vitro activity in acute myeloid blasts and leukemic progenitor cells through the induction of a DNA damage response.
    Haematologica. 96: 687-95.
  2. Amoury, M. et al. (2016) A novel approach for targeted elimination of CSPG4-positive triple-negative breast cancer cells using a MAP tau-based fusion protein.
    Int J Cancer. 139 (4): 916-27.
  3. Maiso, P. et al. (2008) The insulin-like growth factor-I receptor inhibitor NVP-AEW541 provokes cell cycle arrest and apoptosis in multiple myeloma cells.
    Br J Haematol. 141 (4): 470-82.
  4. Cascone, A. et al. (2012) Destabilization of the outer and inner mitochondrial membranes by core and linker histones.
    PLoS One. 7 (4): e35357.
  5. Álvarez-Fernández S et al. (2013) Potent antimyeloma activity of a novel ERK5/CDK inhibitor.
    Clin Cancer Res. 19 (10): 2677-87.
  6. Ortiz-Ruiz, M.J. et al. (2014) Therapeutic potential of ERK5 targeting in triple negative breast cancer.
    Oncotarget. 5 (22): 11308-18.
  7. Ocio, E.M. et al. (2009) Zalypsis: a novel marine-derived compound with potent antimyeloma activity that reveals high sensitivity of malignant plasma cells to DNA double-strand breaks.
    Blood. 113 (16): 3781-91.

Further Reading

  1. Li, L.Y. et al. (2001) Endonuclease G is an apoptotic DNase when released from mitochondria.
    Nature. 412 (6842): 95-9.

Western Blotting

RRID
AB_324300
UniProt
Q14249
Entrez Gene
ENDOG
GO Terms
GO:0003676 nucleic acid binding
GO:0005739 mitochondrion
GO:0004519 endonuclease activity
GO:0046872 metal ion binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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