EEF1G antibody

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Rabbit anti EEF1G

Rabbit anti human EEF1G antibody recognizes eukaryotic elongation factor 1-gamma, also known as EF-1-gamma, PRO1608, eEF-1B gamma, or elongation factor 1-gamma. This antibody has been extensively validated for western blotting using whole cell lysates.

EEF1G Antibody with Control Lysate

Rabbit anti human EEF1G antibody recognizes eukaryotic elongation factor 1-gamma, also known as EF-1-gamma, PRO1608, eEF-1B gamma, elongation factor 1-gamma, pancreatic tumor-related protein or translation elongation factor eEF-1 gamma chain.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00305 WB 100 µl
VPA00305KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human EEF1G antibody recognizes EEF1G, also known as EF-1-gamma, PRO1608, eEF-1B gamma, elongation factor 1-gamma, pancreatic tumor-related protein and translation elongation factor eEF-1 gamma chain.

The EEF1G gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multi-subunit complexes containing this elongation factor and aminoacyl-tRNA synthetases (provided by RefSeq, Jul 2008).

Rabbit anti Human EEF1G antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 208-237 of human EEF1G

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P26641 Related reagents
Entrez Gene
EEF1G Related reagents
GO Terms
GO:0005515 protein binding
GO:0003746 translation elongation factor activity
GO:0009615 response to virus
GO:0005829 cytosol
GO:0005853 eukaryotic translation elongation factor 1 complex
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of EEF1G antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti EEF1G detects a band of approximately 50 kDa in HEK293 cell lysate
Western Blotting
Anti EEF1G detects a band of approximately 50 kDa in HEK293 cell lysate
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB