EEF1G antibody
Rabbit anti EEF1G
Rabbit anti human EEF1G antibody recognizes eukaryotic elongation factor 1-gamma, also known as EF-1-gamma, PRO1608, eEF-1B gamma, or elongation factor 1-gamma. This antibody has been extensively validated for western blotting using whole cell lysates.
EEF1G Antibody with Control Lysate
Rabbit anti human EEF1G antibody recognizes eukaryotic elongation factor 1-gamma, also known as EF-1-gamma, PRO1608, eEF-1B gamma, elongation factor 1-gamma, pancreatic tumor-related protein or translation elongation factor eEF-1 gamma chain.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00305 | WB | 100 µl | ||||
| VPA00305KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human EEF1G antibody recognizes EEF1G, also known as EF-1-gamma, PRO1608, eEF-1B gamma, elongation factor 1-gamma, pancreatic tumor-related protein and translation elongation factor eEF-1 gamma chain.
The EEF1G gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multi-subunit complexes containing this elongation factor and aminoacyl-tRNA synthetases (provided by RefSeq, Jul 2008).
Rabbit anti Human EEF1G antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The EEF1G gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multi-subunit complexes containing this elongation factor and aminoacyl-tRNA synthetases (provided by RefSeq, Jul 2008).
Rabbit anti Human EEF1G antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti EEF1G detects a band of approximately 50 kDa in HEK293 cell lysate
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH-conjugated synthetic peptide corresponding to aa 208-237 of human EEF1G
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P26641 Related reagents
- Entrez Gene
- EEF1G Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0003746 translation elongation factor activity
- GO:0009615 response to virus
- GO:0005829 cytosol
- GO:0005853 eukaryotic translation elongation factor 1 complex
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of EEF1G antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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