DNA PKcs antibody

Rabbit anti DNA PKcs

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
DNA PKcs

Product Code Applications Pack Size List Price Your Price Qty
AHP318
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SDS Safety Datasheet SDS
GS IP P * WB 0.1 ml loader
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loader
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Rabbit anti DNA PKcs antibody recognizes the human DNA-dependent protein kinase catalytic subunit, also known as DNA-PKcs, DNPK1 or p460. DNA PKcs is a 4128 amino acid ~460 kDa nuclear protein encoded by the PRKDC gene, acting as a molecular sensor for DNA damage. Mutation of the PRKDC gene can result in Immunodeficiency 26 with or without neurologic abnormalities (IMD26) (van der Burg et al. 2009).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Hamster
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to DNA-PKcs were raised by repeated immunisation of rabbit with highly purified antigen. Whole serum is supplied.
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Human DNA-PKcs fragment.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Gel Super Shift Assays
Immunohistology - Paraffin 1 1/100
Immunoprecipitation
Western Blotting 1/2000
  1. 1This product requires heat mediated treatment of paraffin sections. Bio-Rad recommend the use of antigen unmasking fluid for this purpose. A two hour incubation with AHP318 is recommended.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for DNA PKcs antibody

  1. Finnie, N.J. et al. (1995) DNA-dependent protein kinase activity is absent in xrs-6 cells: implications for site-specific recombination and DNA double-strand break repair.
    Proc Natl Acad Sci U S A. 92 (1): 320-4.
  2. Jayaram, S. et al. (2008) E1B 55k-independent dissociation of the DNA ligase IV/XRCC4 complex by E4 34k during adenovirus infection.
    Virology.382: 163-70.
  3. Hoek, M. et al. (2011) An analysis of CAF-1-interacting proteins reveals dynamic and direct interactions with the KU complex and 14-3-3 proteins.
    J Biol Chem. 286 (12): 10876-87.
  4. Frasca, D. et al. (2001) Role of DNA-dependent protein kinase in recognition of radiation-induced DNA damage in human peripheral blood mononuclear cells.
    Int Immunol. 13: 791-7.
  5. Jayaram, S. et al. (2008) Loss of DNA ligase IV prevents recognition of DNA by double-strand break repair proteins XRCC4 and XLF.
    Nucleic Acids Res. 36: 5773-86.
  6. Koike, M. et al. (1999) Differential subcellular localization of DNA-dependent protein kinase components Ku and DNA-PKcs during mitosis.
    J Cell Sci. 112: 4031-9.
  7. Yee, M.C. et al. (2005) A cell-permeable, activity-based probe for protein and lipid kinases.
    J Biol Chem. 280: 29053-9.
  8. Zhao, H.J. et al. (2000) DNA-dependent protein kinase activity correlates with Ku70 expression and radiation sensitivity in esophageal cancer cell lines.
    Clin Cancer Res. 6: 1073-8.
  9. View The Latest Product References
  10. Sirzen, F. et al. (1999) DNA-dependent protein kinase content and activity in lung carcinoma cell lines: correlation with intrinsic radiosensitivity.
    Eur J Cancer. 35: 111-6.
  11. Frasca, D. et al. (2002) The DNA repair protein ku is involved in gp130-mediated signal transduction events in PBMC from young but not from elderly subjects.
    Exp Gerontol. 37: 321-8.
  12. Scarpaci, S. et al. (2003) DNA damage recognition and repair capacities in human naïve and memory T cells from peripheral blood of young and elderly subjects.
    Mech Ageing Dev. 124: 517-24.
  13. Kamachi, M. et al. (2002) Human autoimmune sera as molecular probes for the identification of an autoantigen kinase signaling pathway.
    J Exp Med. 196: 1213-25.
  14. Gaymes, T.J. et al. (2006) Histone deacetylase inhibitors (HDI) cause DNA damage in leukemia cells: a mechanism for leukemia-specific HDI-dependent apoptosis?
    Mol Cancer Res. 4 (8): 563-73.
  15. Koike, M. et al. (1999) Differential subcellular localization of DNA-dependent protein kinase components Ku and DNA-PKcs during mitosis.
    J Cell Sci. 112 ( Pt 22): 4031-9.
  16. Nilsson, A. et al. (1999) Cell cycle-dependent regulation of the DNA-dependent protein kinase.
    Cell Prolif. 32 (4): 239-48.
  17. Yumoto Y et al. (1998) High mobility group proteins 1 and 2 can function as DNA-binding regulatory components for DNA-dependent protein kinase in vitro.
    J Biochem. 124 (3): 519-27.

Immunoprecipitation

Western Blotting

RRID
AB_322027
UniProt
P78527
Entrez Gene
PRKDC
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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