DNA PKcs antibody
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Rabbit anti DNA PKcs antibody recognizes the human DNA-dependent protein kinase catalytic subunit, also known as DNA-PKcs, DNPK1 or p460. DNA PKcs is a 4128 amino acid ~460 kDa nuclear protein encoded by the PRKDC gene, acting as a molecular sensor for DNA damage. Mutation of the PRKDC gene can result in Immunodeficiency 26 with or without neurologic abnormalities (IMD26) (van der Burg et al. 2009).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Hamster Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to DNA-PKcs were raised by repeated immunisation of rabbit with highly purified antigen. Whole serum is supplied.
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Human DNA-PKcs fragment.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P78527 Related reagents
- Entrez Gene
- PRKDC Related reagents
- Regulatory
- For research purposes only
Applications of DNA PKcs antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Gel Super Shift Assays | |||
| Immunohistology - Paraffin 1 | 1/100 | ||
| Immunoprecipitation | |||
| Western Blotting | 1/2000 |
- 1This product requires heat mediated treatment of paraffin sections. Bio-Rad recommend the use of antigen unmasking fluid for this purpose. A two hour incubation with AHP318 is recommended.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
Product Specific References
References for DNA PKcs antibody
-
Finnie, N.J. et al. (1995) DNA-dependent protein kinase activity is absent in xrs-6 cells: implications for site-specific recombination and DNA double-strand break repair.
Proc Natl Acad Sci U S A. 92 (1): 320-4. -
Jayaram, S. et al. (2008) E1B 55k-independent dissociation of the DNA ligase IV/XRCC4 complex by E4 34k during adenovirus infection.
Virology.382: 163-70. -
Hoek, M. et al. (2011) An analysis of CAF-1-interacting proteins reveals dynamic and direct interactions with the KU complex and 14-3-3 proteins.
J Biol Chem. 286 (12): 10876-87. -
Frasca, D. et al. (2001) Role of DNA-dependent protein kinase in recognition of radiation-induced DNA damage in human peripheral blood mononuclear cells.
Int Immunol. 13: 791-7. -
Jayaram, S. et al. (2008) Loss of DNA ligase IV prevents recognition of DNA by double-strand break repair proteins XRCC4 and XLF.
Nucleic Acids Res. 36: 5773-86. -
Koike, M. et al. (1999) Differential subcellular localization of DNA-dependent protein kinase components Ku and DNA-PKcs during mitosis.
J Cell Sci. 112: 4031-9. -
Yee, M.C. et al. (2005) A cell-permeable, activity-based probe for protein and lipid kinases.
J Biol Chem. 280: 29053-9. -
Zhao, H.J. et al. (2000) DNA-dependent protein kinase activity correlates with Ku70 expression and radiation sensitivity in esophageal cancer cell lines.
Clin Cancer Res. 6: 1073-8. -
Sirzen, F. et al. (1999) DNA-dependent protein kinase content and activity in lung carcinoma cell lines: correlation with intrinsic radiosensitivity.
Eur J Cancer. 35: 111-6. -
Frasca, D. et al. (2002) The DNA repair protein ku is involved in gp130-mediated signal transduction events in PBMC from young but not from elderly subjects.
Exp Gerontol. 37: 321-8. -
Scarpaci, S. et al. (2003) DNA damage recognition and repair capacities in human naïve and memory T cells from peripheral blood of young and elderly subjects.
Mech Ageing Dev. 124: 517-24. -
Kamachi, M. et al. (2002) Human autoimmune sera as molecular probes for the identification of an autoantigen kinase signaling pathway.
J Exp Med. 196: 1213-25. -
Gaymes, T.J. et al. (2006) Histone deacetylase inhibitors (HDI) cause DNA damage in leukemia cells: a mechanism for leukemia-specific HDI-dependent apoptosis?
Mol Cancer Res. 4 (8): 563-73. -
Koike, M. et al. (1999) Differential subcellular localization of DNA-dependent protein kinase components Ku and DNA-PKcs during mitosis.
J Cell Sci. 112 ( Pt 22): 4031-9. -
Nilsson, A. et al. (1999) Cell cycle-dependent regulation of the DNA-dependent protein kinase.
Cell Prolif. 32 (4): 239-48. -
Yumoto Y et al. (1998) High mobility group proteins 1 and 2 can function as DNA-binding regulatory components for DNA-dependent protein kinase in vitro.
J Biochem. 124 (3): 519-27.
