Browsing Modes for Business Users

DNA PKcs antibody

ISO 9001 Quality Assurance Manufacturing | Print
Anti DNA PKcs Antibody thumbnail image 1
Anti DNA PKcs Antibody gallery image 1
Western blot analysis of HeLa whole cell lysates (reduced, 25ug/lane) probed with Rabbit anti DNA PKcs antibody ( AHP318) at a 1/2000 dilution followed by detection with HRP-conjugated Goat anti Rabbit IgG (H/L) (STAR208P) at 1/10,000 (lane 1). STAR208P only was included as a secondary only control at a 1/10000 dilution (lane 2).

Filter by Application:

WB

Rabbit anti DNA PKcs

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
DNA PKcs

Quick Links:

 Antibody Quality and Performance Guarantee
 Unrivaled Technical Support
Product Code Applications Pack Size List Price Your Price Qty
AHP318
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
GS IP P * WB 0.1 ml
List Price Your Price

Rabbit anti DNA PKcs antibody recognizes the human DNA-dependent protein kinase catalytic subunit, also known as DNA-PKcs, DNPK1 or p460. DNA PKcs is a 4128 amino acid ~460 kDa nuclear protein encoded by the PRKDC gene, acting as a molecular sensor for DNA damage. Mutation of the PRKDC gene can result in Immunodeficiency 26 with or without neurologic abnormalities (IMD26) (van der Burg et al. 2009).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Hamster
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to DNA-PKcs were raised by repeated immunisation of rabbit with highly purified antigen. Whole serum is supplied.
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Human DNA-PKcs fragment.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Gel Super Shift Assays
Immunohistology - Paraffin 1 1/100
Immunoprecipitation
Western Blotting 1/2000
  1. 1This product requires heat mediated treatment of paraffin sections. Bio-Rad recommend the use of antigen unmasking fluid for this purpose. A two hour incubation with AHP318 is recommended.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg
List Price Your Price
Description Goat anti Rabbit IgG (Fc):Biotin
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg
List Price Your Price
Description Goat anti Rabbit IgG (Fc):FITC
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg
List Price Your Price
Description Goat anti Rabbit IgG (Fc):HRP
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg
List Price Your Price
Description Goat anti Rabbit IgG (H/L):HRP
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg
List Price Your Price
Description Sheep anti Rabbit IgG:FITC
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml
List Price Your Price
Description Sheep anti Rabbit IgG:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
List Price Your Price
Description TidyBlot Western Blot Detection Reagent:HRP

References for DNA PKcs antibody

  1. Yumoto Y et al. (1998) High mobility group proteins 1 and 2 can function as DNA-binding regulatory components for DNA-dependent protein kinase in vitro.
    J Biochem. 124 (3): 519-27.
  2. Nilsson, A. et al. (1999) Cell cycle-dependent regulation of the DNA-dependent protein kinase.
    Cell Prolif. 32 (4): 239-48.
  3. Sirzen, F. et al. (1999) DNA-dependent protein kinase content and activity in lung carcinoma cell lines: correlation with intrinsic radiosensitivity.
    Eur J Cancer. 35: 111-6.
  4. Koike, M. et al. (1999) Differential subcellular localization of DNA-dependent protein kinase components Ku and DNA-PKcs during mitosis.
    J Cell Sci. 112: 4031-9.
  5. Zhao, H.J. et al. (2000) DNA-dependent protein kinase activity correlates with Ku70 expression and radiation sensitivity in esophageal cancer cell lines.
    Clin Cancer Res. 6: 1073-8.
  6. Frasca, D. et al. (2001) Role of DNA-dependent protein kinase in recognition of radiation-induced DNA damage in human peripheral blood mononuclear cells.
    Int Immunol. 13: 791-7.
  7. Kamachi, M. et al. (2002) Human autoimmune sera as molecular probes for the identification of an autoantigen kinase signaling pathway.
    J Exp Med. 196: 1213-25.
  8. Frasca, D. et al. (2002) The DNA repair protein ku is involved in gp130-mediated signal transduction events in PBMC from young but not from elderly subjects.
    Exp Gerontol. 37: 321-8.
    9. View The Latest Product References
  9. Scarpaci, S. et al. (2003) DNA damage recognition and repair capacities in human naïve and memory T cells from peripheral blood of young and elderly subjects.
    Mech Ageing Dev. 124: 517-24.
  10. Yee, M.C. et al. (2005) A cell-permeable, activity-based probe for protein and lipid kinases.
    J Biol Chem. 280: 29053-9.
  11. Gaymes, T.J. et al. (2006) Histone deacetylase inhibitors (HDI) cause DNA damage in leukemia cells: a mechanism for leukemia-specific HDI-dependent apoptosis?
    Mol Cancer Res. 4 (8): 563-73.
  12. Jayaram, S. et al. (2008) Loss of DNA ligase IV prevents recognition of DNA by double-strand break repair proteins XRCC4 and XLF.
    Nucleic Acids Res. 36: 5773-86.
  13. Jayaram, S. et al. (2008) E1B 55k-independent dissociation of the DNA ligase IV/XRCC4 complex by E4 34k during adenovirus infection.
    Virology.382: 163-70.
  14. Hoek, M. et al. (2011) An analysis of CAF-1-interacting proteins reveals dynamic and direct interactions with the KU complex and 14-3-3 proteins.
    J Biol Chem. 286 (12): 10876-87.

Further Reading

  1. Finnie, N.J. et al. (1995) DNA-dependent protein kinase activity is absent in xrs-6 cells: implications for site-specific recombination and DNA double-strand break repair.
    Proc Natl Acad Sci U S A. 92 (1): 320-4.

Immunoprecipitation

Western Blotting

RRID
AB_322027
UniProt
P78527
Entrez Gene
PRKDC

AHP318

150191 161516

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with DNA PKCS specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products
Please note