DNA Methyltransferase 1 antibody

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Rabbit anti DNA Methyltransferase 1

DNA methyltransferase 1 antibody recognizes DNA (cytosine-5)-methyltransferase 1 , also known as Dnmt1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, DNA MTase HsaI or M.HsaI MCMT. The antibody has been extensively validated for western blotting using whole cell lysates.

DNA Methyltransferase 1 Antibody with Control Lysate

Product Type: PrecisionAb™ Polyclonal
Isotype: Polyclonal IgG
Format: Purified

Product Code	Applications	Pack Size
VPA00026	WB	100 µl
VPA00026KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Rabbit anti Human DNA methyltransferase 1 antibody recognizes human DNA (cytosine-5) methyltransferase 1, also known as DNMT1, CXXC-type zinc finger protein 9, MCMT or DNA methyltransferase HsaI. DNA methyltransferase 1 is a 1616 amino acid member of the C5-methyltransferase family, bearing 2 BAH domains and single CXXC-type zinc finger, DMAP-interaction and SAM-dependent MTase C5-type domains.The epitope recognized by Rabbit anti Human DNA methyltransferase 1 antibody maps to a region between residues 700 and 750 of human DNMT1.

DNMT1 is a nuclear enzyme with a major role in the maintenance of DNA methylation patterns after replication (Song et al. 2012). DNA methylation is significant for epigenetic regulation of gene expression, X chromosome inactivation, genomic imprinting, and development (Lee et al. 2001). Overexpression of DNMT1 has been detected in several human cancers (Robertson et al. 1999).

Rabbit anti Human DNA methyltransferase 1 antibody recognizes human DNA methyltransferase 1 as a band of ~180 kDa in multiple human cell lysates by western blotting under reducing conditions.

Product Details

Target Species: Human
Western Blotting: Anti DNA Methyltransferase 1 antibody detects a band of approximately 180 kDa in HEK293 cell lysates.
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography
Preparation: 20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution: TRIS-citrate/Phosphate buffered saline
Buffer Solution: TRIS-citrate/Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Synthetic peptide corresponding to a region within human DNMT1 encoded within exon 25
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P26358 Related reagents
Entrez Gene: DNMT1 Related reagents
GO Terms: GO:0000122 negative regulation of transcription from RNA polymerase II promoter; GO:0003677 DNA binding; GO:0003886 DNA (cytosine-5-)-methyltransferase activity; GO:0005634 nucleus; GO:0006350 transcription; GO:0008134 transcription factor binding; GO:0010216 maintenance of DNA methylation; GO:0010628 positive regulation of gene expression; GO:0016568 chromatin modification; GO:0051571 positive regulation of histone H3-K4 methylation; GO:0051573 negative regulation of histone H3-K9 methylation
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of DNA Methyltransferase 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000
Western Blotting			1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Rabbit IgG (H/L):HRP	STAR208P	2 ml	WB
Goat anti Rabbit IgG (H/L):HRP	STAR208P	2 ml	WB

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