DNA Methyltransferase 1 antibody

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Rabbit anti DNA Methyltransferase 1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00026
Datasheet
WB
SDS
100 µl loader

Rabbit anti Human DNA methyltransferase 1 antibody recognizes human DNA (cytosine-5) methyltransferase 1, also known as DNMT1, CXXC-type zinc finger protein 9, MCMT or DNA methyltransferase HsaI. DNA methyltransferase 1 is a 1616 amino acid member of the C5-methyltransferase family, bearing 2 BAH domains and single CXXC-type zinc finger, DMAP-interaction and SAM-dependent MTase C5-type domains. The epitope recognized by Rabbit anti Human DNA methyltransferase 1 antibody maps to a region between residues 700 and 750 of human DNMT1.

DNMT1 is a nuclear enzyme with a major role in the maintenance of DNA methylation patterns after replication (Song et al. 2012). DNA methylation is significant for epigenetic regulation of gene expression, X chromosome inactivation, genomic imprinting, and development (Lee et al. 2001). Overexpression of DNMT1 has been detected in several human cancers (Robertson et al. 1999).

Rabbit anti Human DNA methyltransferase 1 antibody recognizes human DNA methyltransferase 1 as a band of ~180 kDa in multiple human cell lysates by western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti DNA Methyltransferase 1 antibody detects a band of approximately 180 kDa in HEK293 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS-citrate/Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to a region within human DNMT1 encoded within exon 25

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P26358
Entrez Gene
DNMT1
GO Terms
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0003677 DNA binding
GO:0003886 DNA (cytosine-5-)-methyltransferase activity
GO:0005634 nucleus
GO:0006350 transcription
GO:0008134 transcription factor binding
GO:0010216 maintenance of DNA methylation
GO:0010628 positive regulation of gene expression
GO:0016568 chromatin modification
GO:0051571 positive regulation of histone H3-K4 methylation
GO:0051573 negative regulation of histone H3-K9 methylation
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of DNA Methyltransferase 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml