DJ-1 antibody

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Rabbit anti DJ-1

Rabbit anti human DJ-1 antibody recognizes the protein DJ-1, also known as Parkinson disease (autosomal recessive, early onset) 7, PARK7, epididymis secretory sperm binding protein Li 67p or oncogene DJ1. This antibody has been extensively validated for western blotting using whole cell lysates.

DJ-1 Antibody with Control Lysate

Rabbit anti human DJ-1 antibody recognizes the protein DJ-1, also known as Parkinson disease (autosomal recessive, early onset) 7, PARK7, epididymis secretory sperm binding protein Li 67p or oncogene DJ1. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00325 WB 100 µl
VPA00325KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human DJ-1 antibody recognizes the oncogene DJ-1, also known as Parkinson disease (autosomal recessive, early onset) 7, PARK-7 and epididymis secretory sperm binding protein Li 67p

Encoded by the PARK7 gene, oncogene DJ-1 belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in PARK7 gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for PARK7 (provided by RefSeq, Jul 2008).

Rabbit anti Human DJ-1 antibody detects a band of 22 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 130-164 of human DJ-1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q99497 Related reagents
Entrez Gene
PARK7 Related reagents
GO Terms
GO:0005515 protein binding
GO:0005634 nucleus
GO:0008219 cell death
GO:0032091 negative regulation of protein binding
GO:0060765 regulation of androgen receptor signaling pathway
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of DJ-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti DJ-1 detects a band of approximately 22 kDa in HeLa cell lysate
Western Blotting
Anti DJ-1 detects a band of approximately 22 kDa in HeLa cell lysate
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB