DISC1 antibody
Rabbit anti DISC1 antibody (AHP1446) used for the detection of DISC1 in mouse photoreceptors by immunofluorescence.
Image caption:
Colocalization of various Pericentrin interaction partners at the basal body complex of mouse photoreceptors. (A) Scheme of a vertebrate rod photoreceptor. (B–F) Micrographs of a vertical cryostat section through an adult mouse retina double labeled for CG-NAP (B, green) and Cen3 (C, red) as a marker for the ciliary apparatus (the connecting cilium (CC) and the basal body complex (BBC)) and the centrosomes. (B) CG-NAP and (C) Cen3 are localized in the ciliary region of the photoreceptors and at the centrosomes of the other retinal cells. (D) The merge of the stainings with the additional DAPI nuclear staining (blue) shows the partial colocalization of CG-NAP and Cen3 in the ciliary region of the photoreceptors and the centrosomes of the other retinal cells. (E–F) High power views showing the localization of CG-NAP at the BBC of the connecting cilium (E) and at the centrosomes of the other retinal cells (F). (G) Scheme of the ciliary apparatus. (H–K) High power views showing the localization of the Pcnt interaction partners PCM1 (H, green), DISC1 (I, green), IFT20 (J, green), and PC2 (K, green) at the BBC of the connecting cilium of the photoreceptor. Cen3 (red) marks the whole cilium with the ciliary apparatus. RPE: retinal pigment epithel; OS: outer segment; IS: inner segment; ONL: outer nuclear layer; OPL: outer plexiform layer; BB: basal body; CC: connecting cilium; C: centriole; CP: calycal process; MTs: microtubules; N: nucleus; S: synapse. Scale bars: 10 µm (B), 1 µm (E, F, J, K, H, I).
From: Mühlhans J, Brandstätter JH, Gießl A (2011) The Centrosomal Protein Pericentrin Identified at the Basal Body Complex of the Connecting Cilium in Mouse Photoreceptors.
PLoS ONE 6(10): e26496. doi:10.1371/journal.pone.0026496
Rabbit anti DISC1 antibody recognizes disrupted in schizophrenia 1 (DISC1), ~93 kDa protein located in the nucleus, cytoplasm and mitochondria. The DISC1 protein is widely expressed in normal tissues. It is involved in neurite outgrowth and cortical development through its interaction with a variety of proteins including lissencephaly-1 (LIS1), NudeE-like (NUDEL) and phosphodiesterase 4B.
In certain cases of schizophrenia the DISC1 gene has been found to be disrupted by a t(1;11) (q42.1;q14.3) translocation, and studies suggest that DISC1 could be a candidate susceptibility gene for this condition.
Rabbit anti DISC1 antibody has been found suitable for immunocytochemistry (ICC) at a concentration of 5μg/ml.
In certain cases of schizophrenia the DISC1 gene has been found to be disrupted by a t(1;11) (q42.1;q14.3) translocation, and studies suggest that DISC1 could be a candidate susceptibility gene for this condition.
Rabbit anti DISC1 antibody has been found suitable for immunocytochemistry (ICC) at a concentration of 5μg/ml.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Antisera to human DISC1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide - Immunogen
- An 18 amino acid peptide located near the centre of human DISC1.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
Applications of DISC1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | |||
| Western Blotting | 1 | 2ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1446 detects bands of approximately 95-100kDa in SK-N-SH cell lysate.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for DISC1 antibody
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Mühlhans J et al. (2011) The centrosomal protein pericentrin identified at the basal body complex of the connecting cilium in mouse photoreceptors.
PLoS One. 6 (10): e26496.
Further Reading
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Taya, S. et al. (2007) DISC1 regulates the transport of the NUDEL/LIS1/14-3-3epsilon complex through kinesin-1.
J Neurosci. 27 (1): 15-26.
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