DISC1 antibody

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Rabbit anti DISC1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1446
Datasheet
C WB
MSDS
0.1 mg loader

Rabbit anti DISC1 antibody recognizes disrupted in schizophrenia 1 (DISC1), ~93 kDa protein located in the nucleus, cytoplasm and mitochondria. The DISC1 protein is widely expressed in normal tissues. It is involved in neurite outgrowth and cortical development through its interaction with a variety of proteins including lissencephaly-1 (LIS1), NudeE-like (NUDEL) and phosphodiesterase 4B.

In certain cases of schizophrenia the DISC1 gene has been found to be disrupted by a t(1;11) (q42.1;q14.3) translocation, and studies suggest that DISC1 could be a candidate susceptibility gene for this condition.

Rabbit anti DISC1 antibody has been found suitable for immunocytochemistry (ICC) at a concentration of 5μg/ml.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Antisera to human DISC1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
An 18 amino acid peptide located near the centre of human DISC1.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
Q9NRI5 Related reagents
Entrez Gene
DISC1 Related reagents
Regulatory
For research purposes only

Applications of DISC1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Western Blotting 1 2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1446 detects bands of approximately 95-100kDa in SK-N-SH cell lysate.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B C E P WB 1 ml loader
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

References for DISC1 antibody

  1. Mühlhans J et al. (2011) The centrosomal protein pericentrin identified at the basal body complex of the connecting cilium in mouse photoreceptors.
    PLoS One. 6 (10): e26496.

Further Reading

  1. Taya, S. et al. (2007) DISC1 regulates the transport of the NUDEL/LIS1/14-3-3epsilon complex through kinesin-1.
    J Neurosci. 27 (1): 15-26.