Disabled Homolog 2 antibody

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Rabbit anti Disabled Homolog 2

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00208
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human disabled homolog 2 antibody recognizes disabled homolog 2, also known as differentially-expressed protein 2 and mitogen-responsive phosphoprotein.

Encoded by the DAB2 gene, disabled homolog 2 is a mitogen-responsive phosphoprotein. It is expressed in normal ovarian epithelial cells, but is down-regulated or absent from ovarian carcinoma cell lines, suggesting its role as a tumor suppressor. This protein binds to the SH3 domains of GRB2, an adaptor protein that couples tyrosine kinase receptors to SOS (a guanine nucleotide exchange factor for Ras), via its C-terminal proline-rich sequences, and may thus modulate growth factor/Ras pathways by competing with SOS for binding to GRB2. Alternatively spliced transcript variants encoding different isoforms have been found for DAB2 (provided by RefSeq, Oct 2011).

Rabbit anti Human disabled homolog 2 antibody detects a band of 96 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti disabled homolog 2 detects a band of approximately 96 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide between 743-770 amino acids from the C-terminal region of human disabled homolog 2

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P98082
Entrez Gene
DAB2
GO Terms
GO:0001934 positive regulation of protein phosphorylation
GO:0005905 coated pit
GO:0008022 protein C-terminus binding
GO:0008283 cell proliferation
GO:0030665 clathrin coated vesicle membrane
GO:0032091 negative regulation of protein binding
GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0045892 negative regulation of transcription, DNA-dependent
GO:0045893 positive regulation of transcription, DNA-dependent
GO:0090090 negative regulation of canonical Wnt receptor signaling pathway
GO:2000096 positive regulation of Wnt receptor signaling pathway, planar cell polarity pathway
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Disabled Homolog 2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml