Delta-Like Protein 4 antibody

Rabbit anti Human Delta-Like Protein 4

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Delta-Like Protein 4

Product Code Applications Pack Size List Price Your Price Qty
AHP1274
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E P WB 50 µg loader
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Rabbit anti Human Delta-like Protein 4 antibody detects human Delta-like protein 4 (DLL4), also known as Delta-4. DLL4 is a 685 amino acid single pass type 1 transmembrane glycoprotein of ~72 kDa containing a single DSL domain and eight EGF-like domains. Human DLL4 is the homologue of the Drosophila delta protein, and functions as a transmembrane bound ligand to the Notch receptor, Notch1.

DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by vascular endothelial growth factor (VEGF), as a negative feedback regulator to regulate angiogenic sprouting and promote the formation of a differentiated vascular network (Mailhos et al. 2001). DLL4 is strongly expressed in tumor vessels of primary renal tumors (Patel et al. 2005), bladder cancer (Patel et al. 2006), and inhibition of DLL4 results in increased vascular proliferation but defective maturation. This in turn leads to a decrease in tumor growth, with no apparent toxicity (Ridgway et al. 2006).

Expression of DLL4 in normal human dermis is low in foetal tissues, becomes more intense during early life (0-20 years) and gradually declines thereafter as shown by immunohistochemisrtry on FFPE tissues using rabbit anti human DLL4 antibody (Gunin et al. 2014).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
Chimpanzee Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to DLL4 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to the internal region of human DLL4.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/3000 1/15000
Immunohistology - Paraffin 5 15ug/ml
Western Blotting 1/300 1/1500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human ovary.
Western Blotting
AHP1274 detects a band of approximately 74kDa in pancreatic cell lysates.

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References for Delta-Like Protein 4 antibody

  1. You, C. et al. (2013) Loss of CCM3 impairs DLL4-Notch signalling: implication in endothelial angiogenesis and in inherited cerebral cavernous malformations.
    J Cell Mol Med. 17 (3): 407-18.
  2. Villaamil, V.M. et al. (2012) Multiple biomarker tissue arrays: A computational approach to identifying protein-protein interactions in the EGFR/ERK signalling pathway.
    J Mol Signal. 7: 14.
  3. Gunin, A.G. et al. (2014) Age-related changes in angiogenesis in human dermis.
    Exp Gerontol. 55C: 143-51.
  4. El Hindy, N. et al. (2013) Implications of Dll4-Notch signaling activation in primary glioblastoma multiforme.
    Neuro Oncol. 15: 1366-78.
  5. Medina Villaamil, V. et al. (2012) Searching for Hif1-α interacting proteins in renal cell carcinoma.
    Clin Transl Oncol. 14: 698-708.
  6. Gunin, A.G. et al. (2014) Age-related changes in angiogenesis in human dermis.
    Exp Gerontol. 55: 143-51.
  7. Hjelmgren O et al. (2016) Increased Vascularization in the Vulnerable Upstream Regions of Both Early and Advanced Human Carotid Atherosclerosis.
    PLoS One. 11 (12): e0166918.

Further Reading

  1. Thurston, G. et al. (2007) The Delta paradox: DLL4 blockade leads to more tumour vessels but less tumour growth.
    Nat Rev Cancer. 7 (5): 327-31.

Immunohistology - Paraffin

Synonyms
DLL4
RRID
AB_2092966
UniProt
Q9NR61
Entrez Gene
DLL4
GO Terms
GO:0005886 plasma membrane
GO:0005509 calcium ion binding
GO:0016021 integral to membrane
GO:0005112 Notch binding
GO:0007154 cell communication
GO:0007219 Notch signaling pathway
GO:0007220 Notch receptor processing
GO:0008015 blood circulation
GO:0030154 cell differentiation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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