Cyclin D1 antibody

Rabbit anti Cyclin D1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Cyclin D1

Product Code Applications Pack Size List Price Your Price Qty
2407-0508
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 ml loader
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Rabbit anti cyclin D1 antibody recognizes Cyclin D1, a member of the cyclin family that function as CKD reculators. Cyclin D1 is involved in cell cycle transition and has been shown to interact with the retinoblastoma protein. The unprocessed precursor is 295 amino acids long. It is expressed in abnormally high levels in a number of cancers and is specifically implicated in breast cancer development.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Dog Expected from Sequence
Chimpanzee Expected from Sequence
Amphibia Expected from Sequence
Zebrafish Expected from Sequence
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Preparation
This antiserum has been delipidated, defibrinated and sterile filtered.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Sodium Azide (NaN3)
Immunogen
Full length synthetic polypeptide corresponding to the human gene sequence.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2,000 1/10,000
Western Blotting 1/500 1/1,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Western Blotting
When used on asynchronous HN30 cell lysates, a band was observed at approximately 34 kDa corresponding to Cyclin D1.

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RRID
AB_2245335
UniProt
P24385
Entrez Gene
CCND1
GO Terms
GO:0000080 G1 phase of mitotic cell cycle
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0000307 cyclin-dependent protein kinase holoenzyme complex
GO:0001934 positive regulation of protein phosphorylation
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0042493 response to drug
GO:0019901 protein kinase binding
GO:0031571 mitotic cell cycle G1/S transition DNA damage checkpoint
GO:0045737 positive regulation of cyclin-dependent protein kinase activity
GO:0051301 cell division
GO:0070141 response to UV-A
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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