Cyclin D1 antibody

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Rabbit anti Cyclin D1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2407-0508 E WB 0.1 ml
Rabbit anti cyclin D1 antibody recognizes Cyclin D1, a member of the cyclin family that function as CKD reculators. Cyclin D1 is involved in cell cycle transition and has been shown to interact with the retinoblastoma protein. The unprocessed precursor is 295 amino acids long. It is expressed in abnormally high levels in a number of cancers and is specifically implicated in breast cancer development.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Dog Expected from Sequence
Chimpanzee Expected from Sequence
Amphibia Expected from Sequence
Zebrafish Expected from Sequence
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Preparation
This antiserum has been delipidated, defibrinated and sterile filtered.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Sodium Azide (NaN3)
Immunogen
Full length synthetic polypeptide corresponding to the human gene sequence.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P24385 Related reagents
Entrez Gene
CCND1 Related reagents
GO Terms
GO:0000080 G1 phase of mitotic cell cycle
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0000307 cyclin-dependent protein kinase holoenzyme complex
GO:0001934 positive regulation of protein phosphorylation
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0042493 response to drug
GO:0019901 protein kinase binding
GO:0031571 mitotic cell cycle G1/S transition DNA damage checkpoint
GO:0045737 positive regulation of cyclin-dependent protein kinase activity
GO:0051301 cell division
GO:0070141 response to UV-A
Regulatory
For research purposes only

Applications of Cyclin D1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2,000 1/10,000
Western Blotting 1/500 1/1,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Western Blotting
When used on asynchronous HN30 cell lysates, a band was observed at approximately 34 kDa corresponding to Cyclin D1.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*
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