Cyclin D1 antibody
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Rabbit anti cyclin D1 antibody recognizes Cyclin D1, a member of the cyclin family that function as CKD reculators. Cyclin D1 is involved in cell cycle transition and has been shown to interact with the retinoblastoma protein. The unprocessed precursor is 295 amino acids long. It is expressed in abnormally high levels in a number of cancers and is specifically implicated in breast cancer development.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Dog Expected from Sequence Chimpanzee Expected from Sequence Amphibia Expected from Sequence Zebrafish Expected from Sequence Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Preparation
- This antiserum has been delipidated, defibrinated and sterile filtered.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.01% Sodium Azide (NaN3) - Immunogen
- Full length synthetic polypeptide corresponding to the human gene sequence.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P24385
- Entrez Gene
- CCND1
- GO Terms
- GO:0000080 G1 phase of mitotic cell cycle
- GO:0000082 G1/S transition of mitotic cell cycle
- GO:0000084 S phase of mitotic cell cycle
- GO:0000307 cyclin-dependent protein kinase holoenzyme complex
- GO:0001934 positive regulation of protein phosphorylation
- GO:0005829 cytosol
- GO:0005654 nucleoplasm
- GO:0042493 response to drug
- GO:0019901 protein kinase binding
- GO:0031571 mitotic cell cycle G1/S transition DNA damage checkpoint
- GO:0045737 positive regulation of cyclin-dependent protein kinase activity
- GO:0051301 cell division
- GO:0070141 response to UV-A
- Regulatory
- For research purposes only
Applications of Cyclin D1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/2,000 | 1/10,000 | |
| Western Blotting | 1/500 | 1/1,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Western Blotting
- When used on asynchronous HN30 cell lysates, a band was observed at approximately 34 kDa corresponding to Cyclin D1.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
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