CX3CR1 antibody

Rabbit anti Human CX3CR1 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CX3CR1
Region
(N-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP1589
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SDS Safety Datasheet SDS
P * WB 0.1 mg loader
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loader
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Rabbit anti Human CX3CR1 antibody recognizes the N-terminal region of Human CX3CR1, a 40.4 kDa seven trans-membrane protein.

CX3CR1, also known as fractalkine receptor, is the receptor for CX3CL1 and acts as a co-receptor with CD4 for the HIV-1 envelope protein. It is expressed in a variety of human tissues and mediates leucocyte adhesion and migration.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human CX3CR1 (NT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to amino acids 2-21 of human CX3CR1.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 2.0ug/ml
Western Blotting 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human heart
Western Blotting
AHP1589 detects a band of approximately 50kDa in human spleen cell lysates.

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References for CX3CR1 antibody

  1. Viladomiu, M. et al. (2017) Cooperation of Gastric Mononuclear Phagocytes with Helicobacter pylori during Colonization.
    J Immunol. 198 (8): 3195-204.
  2. Lorenzini, I. et al. (2020) Activated iPSC-microglia from C9orf72 ALS/FTD patients exhibit endosomal-lysosomal dysfunction
    bioRxiv 2020.09.03.277459. 03 Sep [Epub ahead of print].

Further Reading

  1. Combadiere, C. et al. (1995) Cloning, chromosomal localization, and RNA expression of a human beta chemokine receptor-like gene.
    DNA Cell Biol. 14 (8): 673-80.
  2. Combadiere, C. et al. (1998) Gene cloning, RNA distribution, and functional expression of mCX3CR1, a mouse chemotactic receptor for the CX3C chemokine fractalkine.
    Biochem Biophys Res Commun. 253 (3): 728-32.
  3. Combadiere, C. et al. (1998) Identification of CX3CR1. A chemotactic receptor for the human CX3C chemokine fractalkine and a fusion coreceptor for HIV-1.
    J Biol Chem. 273 (37): 23799-804.
  4. Raport, C.J. et al. (1995) The orphan G-protein-coupled receptor-encoding gene V28 is closely related to genes for chemokine receptors and is expressed in lymphoid and neural tissues.
    Gene. 163 (2): 295-9.

Synonyms
Fractalkine Receptor
RRID
AB_2087421
UniProt
P49238
Entrez Gene
CX3CR1
GO Terms
GO:0007155 cell adhesion
GO:0004950 chemokine receptor activity
GO:0005887 integral to plasma membrane
GO:0006968 cellular defense response
GO:0009611 response to wounding
GO:0044419 interspecies interaction between organisms
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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