CX3CR1 antibody
Rabbit anti Human CX3CR1 antibody (AHP1589) used to label CX3CR1 expressing cells by immunofluorescence.
Image caption:
Healthy control and mutant C9orf72 ALS/FTD patient iPSC lines differentiate into mature microglia.
(C) Representative immunofluorescence of DIV 40 control (n = 3) and C9orf72 ALS/FTD iPSC-MG (n = 3–5) stained for myeloid transcription factor PU.1 and microglia specific markers such as purinergic surface receptor P2ry12, C-X3-C Motif Chemokine Receptor 1 (Cx3cr1), triggering receptor expressed on myeloid cells 2 (TREM2), and the transmembrane protein 119 (TMEM119). Scale bar, 20 μm.
From: Lorenzini I, Alsop E, Levy J, Gittings LM, Lall D, Rabichow BE, Moore S, Pevey R, Bustos LM, Burciu C, Bhatia D, Singer M, Saul J, McQuade A, Tzioras M, Mota TA, Logemann A, Rose J, Almeida S, Gao FB, Marks M, Donnelly CJ, Hutchins E, Hung ST, Ichida J, Bowser R, Spires-Jones T, Blurton-Jones M, Gendron TF, Baloh RH, Van Keuren-Jensen K, Sattler R.
Moderate intrinsic phenotypic alterations in C9orf72 ALS/FTD iPSC-microglia despite the presence of C9orf72 pathological features.
Front Cell Neurosci. 2023 Jun 6;17:1179796.
doi: 10.3389/fncel.2023.1179796.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Human CX3CR1 (N-Terminal)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- CX3CR1
- Region
- (N-TERMINAL)
Antibody Quality and Performance Guarantee
| Rabbit anti Human CX3CR1 antibody recognizes the N-terminal region of Human CX3CR1, a 40.4 kDa seven trans-membrane protein. CX3CR1, also known as fractalkine receptor, is the receptor for CX3CL1 and acts as a co-receptor with CD4 for the HIV-1 envelope protein. It is expressed in a variety of human tissues and mediates leucocyte adhesion and migration. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human CX3CR1 (NT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- A peptide corresponding to amino acids 2-21 of human CX3CR1.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 |  |
2.0ug/ml | |
| Western Blotting | |
0.5 | 1.0ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human heart
- Western Blotting
- AHP1589 detects a band of approximately 50kDa in human spleen cell lysates.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
References for CX3CR1 antibody
-
Viladomiu, M. et al. (2017) Cooperation of Gastric Mononuclear Phagocytes with Helicobacter pylori during Colonization.
J Immunol. 198 (8): 3195-204. -
Lorenzini, I. et al. (2023) Moderate intrinsic phenotypic alterations in C9orf72 ALS/FTD iPSC-microglia despite the presence of C9orf72 pathological features.
Front Cell Neurosci. 17: 1179796.
Further Reading
-
Combadiere, C. et al. (1995) Cloning, chromosomal localization, and RNA expression of a human beta chemokine receptor-like gene.
DNA Cell Biol. 14 (8): 673-80. -
Raport, C.J. et al. (1995) The orphan G-protein-coupled receptor-encoding gene V28 is closely related to genes for chemokine receptors and is expressed in lymphoid and neural tissues.
Gene. 163 (2): 295-9. -
Combadiere, C. et al. (1998) Gene cloning, RNA distribution, and functional expression of mCX3CR1, a mouse chemotactic receptor for the CX3C chemokine fractalkine.
Biochem Biophys Res Commun. 253 (3): 728-32. -
Combadiere, C. et al. (1998) Identification of CX3CR1. A chemotactic receptor for the human CX3C chemokine fractalkine and a fusion coreceptor for HIV-1.
J Biol Chem. 273 (37): 23799-804.
- Synonyms
- Fractalkine Receptor
- RRID
- AB_2087421
- UniProt
- P49238
- Entrez Gene
- CX3CR1
- GO Terms
- GO:0007155 cell adhesion
- GO:0004950 chemokine receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006968 cellular defense response
- GO:0009611 response to wounding
- GO:0044419 interspecies interaction between organisms
AHP1589
148599
158579
163911
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with CX3CR1 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up