CX3CR1 antibody

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Rabbit anti Human CX3CR1 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1589 P* WB 0.1 mg
Rabbit anti Human CX3CR1 antibody recognizes the N-terminal region of Human CX3CR1, a 40.4 kDa seven trans-membrane protein.

CX3CR1, also known as fractalkine receptor, is the receptor for CX3CL1 and acts as a co-receptor with CD4 for the HIV-1 envelope protein. It is expressed in a variety of human tissues and mediates leucocyte adhesion and migration.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human CX3CR1 (NT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to amino acids 2-21 of human CX3CR1.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P49238 Related reagents
Entrez Gene
CX3CR1 Related reagents
GO Terms
GO:0007155 cell adhesion
GO:0004950 chemokine receptor activity
GO:0005887 integral to plasma membrane
GO:0006968 cellular defense response
GO:0009611 response to wounding
GO:0044419 interspecies interaction between organisms
Regulatory
For research purposes only

Applications of CX3CR1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 2.0ug/ml
Western Blotting 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human heart
Western Blotting
AHP1589 detects a band of approximately 50kDa in human spleen cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for CX3CR1 antibody

  1. Viladomiu, M. et al. (2017) Cooperation of Gastric Mononuclear Phagocytes with Helicobacter pylori during Colonization.
    J Immunol. 198 (8): 3195-204.

Further Reading

  1. Combadiere, C. et al. (1995) Cloning, chromosomal localization, and RNA expression of a human beta chemokine receptor-like gene.
    DNA Cell Biol. 14 (8): 673-80.
  2. Combadiere, C. et al. (1998) Gene cloning, RNA distribution, and functional expression of mCX3CR1, a mouse chemotactic receptor for the CX3C chemokine fractalkine.
    Biochem Biophys Res Commun. 253 (3): 728-32.
  3. Combadiere, C. et al. (1998) Identification of CX3CR1. A chemotactic receptor for the human CX3C chemokine fractalkine and a fusion coreceptor for HIV-1.
    J Biol Chem. 273 (37): 23799-804.
  4. Raport, C.J. et al. (1995) The orphan G-protein-coupled receptor-encoding gene V28 is closely related to genes for chemokine receptors and is expressed in lymphoid and neural tissues.
    Gene. 163 (2): 295-9.