CREB3L2 antibody

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Rabbit anti CREB3L2

Rabbit anti human CREB3L2 antibody recognizes cyclic AMP-responsive element-binding protein 3-like 2, also known as B-ZIB transcription factor, BBF2 human homolog on chromosome 7, or basic transcription factor 2. This antibody has been validated for western blotting using whole cell lysates.

CREB3L2 Antibody with Control Lysate

Rabbit anti human CREB3L2 antibody recognizes cyclic AMP-responsive element-binding protein 3-like 2, also known as B-ZIB transcription factor, BBF2 human homolog on chromosome 7, FUS/BBF2H7 protein, TCAG_1951439 or basic transcription factor 2.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00261 WB 100 µl
VPA00261KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human CREB3L2 antibody recognizes CREB3L2, also known as B-ZIB transcription factor, BBF2 human homolog on chromosome 7, FUS/BBF2H7 protein, TCAG_1951439, basic transcription factor 2 and cAMP-responsive element-binding protein 3-like protein 2.

The CREB3L2 gene encodes a member of the OASIS bZIP transcription factor family. Members of this family can dimerize but form homodimers only. The CREB3L2 protein is a transcriptional activator. Translocations between CREB3L2 on chromosome 7 and the gene fused in sarcoma on chromosome 16 can be found in some tumors. Multiple transcript variants encoding different isoforms have been found for CREB3L2 (provided by RefSeq, Dec 2011).

Rabbit anti CREB3L2 antibody detects a band of 57 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti CREB3L2 detects a band of approximately 57 kDa in HEK293 cell lysate
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 490-517 of human CREB3L2
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q70SY1 Related reagents
Entrez Gene
CREB3L2 Related reagents
GO Terms
GO:0002062 chondrocyte differentiation
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0006986 response to unfolded protein
GO:0016021 integral to membrane
GO:0005634 nucleus
GO:0005789 endoplasmic reticulum membrane
GO:0006355 regulation of transcription, DNA-dependent
GO:0034976 response to endoplasmic reticulum stress
GO:0035497 cAMP response element binding
GO:0045941 positive regulation of transcription
GO:0046983 protein dimerization activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of CREB3L2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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