CoREST antibody
Rabbit anti CoREST
Rabbit anti human CoREST antibody recognizes REST (repressor element-1 silencing transcription factor) co-repressor 1, also known as RCOR1. This antibody has been extensively validated for western blotting using whole cell lysates.
CoREST Antibody with Control Lysate
Rabbit anti human CoREST antibody recognizes REST (repressor element-1 silencing transcription factor) co-repressor 1, also known as RCOR1. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00334 | WB | 100 µl | ||||
| VPA00334KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human CoREST antibody recognizes CoREST also known as, REST (repressor element-1 silencing transcription factor) co-repressor 1 or RCOR1.
Encoded by the RCOR1 gene, CoREST is a well-conserved protein, down-regulated at birth, and with a specific role in determining neural cell differentiation. CoREST binds to the C-terminal domain of REST (provided by RefSeq, Aug 2011).
Rabbit anti CoREST antibody detects a band of 65 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the RCOR1 gene, CoREST is a well-conserved protein, down-regulated at birth, and with a specific role in determining neural cell differentiation. CoREST binds to the C-terminal domain of REST (provided by RefSeq, Aug 2011).
Rabbit anti CoREST antibody detects a band of 65 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti CoREST detects a band of approximately 65 kDa in Jurkat cell lysate
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH-conjugated synthetic peptide corresponding to aa 101-130 of human CoREST
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q9UKL0 Related reagents
- Entrez Gene
- RCOR1 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0003677 DNA binding
- GO:0007596 blood coagulation
- GO:0005654 nucleoplasm
- GO:0006350 transcription
- GO:0016568 chromatin modification
- GO:0044419 interspecies interaction between organisms
- GO:0045449 regulation of transcription
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of CoREST antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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