Collagen I antibody
Rabbit anti Human collagen I antibody (2150-0020) used for the evaluation of collagen I expression in chondral repair tissue by immunohistochemistry on formalin fixed paraffin embedded tissue sections
Image caption:
Histological stains. Histological stains of the biopsies of cartilage and underlying bone that were taken from the site of the chondral allograft implantation on the trochlea at nine months following the repair (repair tissue) and from the trochlea of a cadaver (healthy tissue, for comparison). Scale bars = 500 μm.
From: Hoffman JK, Geraghty S, Protzman NM.
Articular cartilage repair using marrow stimulation augmented with a viable chondral allograft: 9-month postoperative histological evaluation.
Case Rep Orthop. 2015;2015:617365.
Rabbit anti Human collagen I antibody (2150-0020) used for the evaluation of collagen I expression in chondral repair tissue by immunohistochemistry on formalin fixed paraffin embedded tissue sections
Image caption:
Histological stains after marrow stimulation. Histological stains of cartilage repair tissue collected from 43-year-old patient 8.2 months after marrow stimulation surgery that required revision. Section outlined in blue in H&E stain is fibrocartilage, while the remaining tissue is primarily hyaline-like cartilage. Scale bars = 500 μm.
From: Hoffman JK, Geraghty S, Protzman NM.
Articular cartilage repair using marrow stimulation augmented with a viable chondral allograft: 9-month postoperative histological evaluation.
Case Rep Orthop. 2015;2015:617365.
Rabbit anti Human collagen I antibody recognizes human Collagen I, reacting with both native and heat denatured proteins, and has been cross-absorbed against Collagen types III, IV and V.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 1% BSA and 0.09% sodium azide is recommended. - Preparation
- Purified IgG prepared by affinity chromatography on immobilized Collagen type I
- Buffer Solution
- 150mM NaCl
10mM Na-Phosphate - Preservative Stabilisers
- 1% Dextran
1% Mannitol - Immunogen
- Collagen type I from human placenta.
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
Applications of Collagen I antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/500 | 1/3000 | |
| Immunohistology - Frozen | 1/10 | 1/50 | |
| Immunohistology - Paraffin 1 | 1/10 | 1/50 |
- 1This product requires antigen retrieval using 1mg/ml pepsin in 0.5M acetic acid for 2 hours at 37oC and multiple buffer washes prior to staining of paraffin sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
Product Specific References
References for Collagen I antibody
-
Evans, M.J. et al. (2002) Fibroblast growth factor-2 during postnatal development of the tracheal basement membrane zone.
Am J Physiol Lung Cell Mol Physiol. 283 (6): L1263-70. -
Kim, H.J. & Im, G.I. (2010) The effects of ERK1/2 inhibitor on the chondrogenesis of bone marrow- and adipose tissue-derived multipotent mesenchymal stromal cells.
Tissue Eng Part A. 16: 851-60. -
Lee, J.S. and Im, G.I. (2010) Influence of chondrocytes on the chondrogenic differentiation of adipose stem cells.
Tissue Eng Part A. 16: 3569-77. -
Kim, Y.J. et al. (2008) PTHrP promotes chondrogenesis and suppresses hypertrophy from both bone marrow-derived and adipose tissue-derived MSCs.
Biochem Biophys Res Commun. 373: 104-8 -
Li M et al. (2013) Short periods of cyclic mechanical strain enhance triple-supplement directed osteogenesis and bone nodule formation by human embryonic stem cells in vitro.
Tissue Eng Part A. 19 (19-20): 2130-7. -
Hoffman, J.K. et al. (2015) Articular cartilage repair using marrow stimulation augmented with a viable chondral allograft: 9-month postoperative histological evaluation.
Case Rep Orthop. 2015: 617365.
