Collagen I antibody

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Rabbit anti Human Collagen I

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2150-0020 C E P* 50 µg
Rabbit anti Human collagen I antibody recognizes human Collagen I, reacting with both native and heat denatured proteins, and has been cross-absorbed against Collagen types III, IV and V.

Product Details

Target Species
Human
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 1% BSA and 0.09% sodium azide is recommended.
Preparation
Purified IgG prepared by affinity chromatography on immobilized Collagen type I
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
1% Dextran
1% Mannitol
Immunogen
Collagen type I from human placenta.

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P02452 Related reagents
P08123 Related reagents
Entrez Gene
COL1A1 Related reagents
COL1A2 Related reagents
Regulatory
For research purposes only

Applications of Collagen I antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/3000
Immunohistology - Frozen 1/10 1/50
Immunohistology - Paraffin 1 1/10 1/50
  1. 1This product requires antigen retrieval using 1mg/ml pepsin in 0.5M acetic acid for 2 hours at 37oC and multiple buffer washes prior to staining of paraffin sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P

Product Specific References

References for Collagen I antibody

  1. Evans, M.J. et al. (2002) Fibroblast growth factor-2 during postnatal development of the tracheal basement membrane zone.
    Am J Physiol Lung Cell Mol Physiol. 283 (6): L1263-70.
  2. Kim, H.J. & Im, G.I. (2010) The effects of ERK1/2 inhibitor on the chondrogenesis of bone marrow- and adipose tissue-derived multipotent mesenchymal stromal cells.
    Tissue Eng Part A. 16: 851-60.
  3. Lee, J.S. and Im, G.I. (2010) Influence of chondrocytes on the chondrogenic differentiation of adipose stem cells.
    Tissue Eng Part A. 16: 3569-77.
  4. Kim, Y.J. et al. (2008) PTHrP promotes chondrogenesis and suppresses hypertrophy from both bone marrow-derived and adipose tissue-derived MSCs.
    Biochem Biophys Res Commun. 373: 104-8
  5. Li M et al. (2013) Short periods of cyclic mechanical strain enhance triple-supplement directed osteogenesis and bone nodule formation by human embryonic stem cells in vitro.
    Tissue Eng Part A. 19 (19-20): 2130-7.
  6. Hoffman, J.K. et al. (2015) Articular cartilage repair using marrow stimulation augmented with a viable chondral allograft: 9-month postoperative histological evaluation.
    Case Rep Orthop. 2015: 617365.