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Collagen I antibody

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Anti Human Collagen I Antibody thumbnail image 1 Anti Human Collagen I Antibody thumbnail image 2
Anti Human Collagen I Antibody gallery image 1
Published customer image:
Rabbit anti Human collagen I antibody (2150-0020) used for the evaluation of collagen I expression in chondral repair tissue by immunohistochemistry on formalin fixed paraffin embedded tissue sections
Image caption:
Histological stains. Histological stains of the biopsies of cartilage and underlying bone that were taken from the site of the chondral allograft implantation on the trochlea at nine months following the repair (repair tissue) and from the trochlea of a cadaver (healthy tissue, for comparison). Scale bars = 500 μm.

From: Hoffman JK, Geraghty S, Protzman NM.
Articular cartilage repair using marrow stimulation augmented with a viable chondral allograft: 9-month postoperative histological evaluation.
Case Rep Orthop. 2015;2015:617365.
Anti Human Collagen I Antibody gallery image 2
Published customer image:
Rabbit anti Human collagen I antibody (2150-0020) used for the evaluation of collagen I expression in chondral repair tissue by immunohistochemistry on formalin fixed paraffin embedded tissue sections
Image caption:
Histological stains after marrow stimulation. Histological stains of cartilage repair tissue collected from 43-year-old patient 8.2 months after marrow stimulation surgery that required revision. Section outlined in blue in H&E stain is fibrocartilage, while the remaining tissue is primarily hyaline-like cartilage. Scale bars = 500 μm.

From: Hoffman JK, Geraghty S, Protzman NM.
Articular cartilage repair using marrow stimulation augmented with a viable chondral allograft: 9-month postoperative histological evaluation.
Case Rep Orthop. 2015;2015:617365.

Rabbit anti Human Collagen I

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
2150-0020
Datasheet
C E P*
SDS
50 µg loader

Rabbit anti Human collagen I antibody recognizes human Collagen I, reacting with both native and heat denatured proteins, and has been cross-absorbed against Collagen types III, IV and V.

Product Details

Target Species
Human
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 1% BSA and 0.09% sodium azide is recommended.
Preparation
Purified IgG prepared by affinity chromatography on immobilized Collagen type I
Buffer Solution
0.15M Sodium Chloride
0.01M Sodium Phosphate
Preservative Stabilisers
1% Mannitol
1% Dextran
Immunogen
Collagen type I from human placenta.

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

UniProt
P02452
P08123
Entrez Gene
COL1A1
COL1A2
Regulatory
For research purposes only

Applications of Collagen I antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/3000
Immunohistology - Frozen 1/10 1/50
Immunohistology - Paraffin 1 1/10 1/50
  1. 1This product requires antigen retrieval using 1mg/ml pepsin in 0.5M acetic acid for 2 hours at 37oC and multiple buffer washes prior to staining of paraffin sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
100x Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml

Product Specific References

References for Collagen I antibody

  1. Evans, M.J. et al. (2002) Fibroblast growth factor-2 during postnatal development of the tracheal basement membrane zone.
    Am J Physiol Lung Cell Mol Physiol. 283 (6): L1263-70.
  2. Kim, H.J. & Im, G.I. (2010) The effects of ERK1/2 inhibitor on the chondrogenesis of bone marrow- and adipose tissue-derived multipotent mesenchymal stromal cells.
    Tissue Eng Part A. 16: 851-60.
  3. Lee, J.S. and Im, G.I. (2010) Influence of chondrocytes on the chondrogenic differentiation of adipose stem cells.
    Tissue Eng Part A. 16: 3569-77.
  4. Kim, Y.J. et al. (2008) PTHrP promotes chondrogenesis and suppresses hypertrophy from both bone marrow-derived and adipose tissue-derived MSCs.
    Biochem Biophys Res Commun. 373: 104-8
  5. Li M et al. (2013) Short periods of cyclic mechanical strain enhance triple-supplement directed osteogenesis and bone nodule formation by human embryonic stem cells in vitro.
    Tissue Eng Part A. 19 (19-20): 2130-7.
  6. Hoffman, J.K. et al. (2015) Articular cartilage repair using marrow stimulation augmented with a viable chondral allograft: 9-month postoperative histological evaluation.
    Case Rep Orthop. 2015: 617365.

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