Rabbit anti Human collagen I antibody recognizes human Collagen I, reacting with both native and heat denatured proteins, and has been cross-absorbed against Collagen types III, IV and V.
- Target Species
- Product Form
- Purified IgG - lyophilized
- Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 1% BSA and 0.09% sodium azide is recommended.
- Purified IgG prepared by affinity chromatography on immobilized Collagen type I
- Buffer Solution
- 0.1M Sodium Chloride
0.01M Sodium Phosphate
0.01M Sodium Borate
- Preservative Stabilisers
- 1% Mannitol
- Collagen type I from human placenta.
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
- Guaranteed until date of expiry. Please see product label.
- Entrez Gene
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Collagen I antibody
|Immunohistology - Frozen
|Immunohistology - Paraffin 1
- 1This product requires antigen retrieval using 1mg/ml pepsin in 0.5M acetic acid for 2 hours at 37oC and multiple buffer washes prior to staining of paraffin sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for Collagen I antibody
Evans, M.J. et al. (2002) Fibroblast growth factor-2 during postnatal development of the tracheal basement membrane zone.
Am J Physiol Lung Cell Mol Physiol. 283 (6): L1263-70.
Kim, H.J. & Im, G.I. (2010) The effects of ERK1/2 inhibitor on the chondrogenesis of bone marrow- and adipose tissue-derived multipotent mesenchymal stromal cells.
Tissue Eng Part A. 16: 851-60.
Lee, J.S. and Im, G.I. (2010) Influence of chondrocytes on the chondrogenic differentiation of adipose stem cells.
Tissue Eng Part A. 16: 3569-77.
Kim, Y.J. et al. (2008) PTHrP promotes chondrogenesis and suppresses hypertrophy from both bone marrow-derived and adipose tissue-derived MSCs.
Biochem Biophys Res Commun. 373: 104-8
Li M et al. (2013) Short periods of cyclic mechanical strain enhance triple-supplement directed osteogenesis and bone nodule formation by human embryonic stem cells in vitro.
Tissue Eng Part A. 19 (19-20): 2130-7.
Hoffman, J.K. et al. (2015) Articular cartilage repair using marrow stimulation augmented with a viable chondral allograft: 9-month postoperative histological evaluation.
Case Rep Orthop. 2015: 617365.
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