Clusterin antibody
Rabbit anti Human clusterin antibody recognizes human clusterin, also known as apolipoprotein J, Ku70-binding protein 1 or TRPM-2. Clusterin is a ubiquitous glycoprotein which exists in at least two distinct isoforms; a proapoptotic nuclear form, of ~49 kDa, and a prosurvival secretory form with a molecular mass of 75-80 kDa. Clusterin is implicated in several diverse physiological processes including sperm maturation, cell differentiation, and apoptosis.
Clusterin upregulation has been reported in various human malignancies, including bladder, prostate, and breast tumours.
Following specific stress induced injury, secretory clusterin acts as a molecular chaperone, scavenging denatured proteins outside cells. Clusterin upregulated in Alzheimer’s disease (Miners et al. 2017), retinitis pigmentosa (Jomary et al 1993), glomerulonephritis, atherosclerosis and myocardial infarction (Rosenberg et al. 2002).
Clusterin upregulation has been reported in various human malignancies, including bladder, prostate, and breast tumours.
Following specific stress induced injury, secretory clusterin acts as a molecular chaperone, scavenging denatured proteins outside cells. Clusterin upregulated in Alzheimer’s disease (Miners et al. 2017), retinitis pigmentosa (Jomary et al 1993), glomerulonephritis, atherosclerosis and myocardial infarction (Rosenberg et al. 2002).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to Clusterin were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide (NaN3) - Immunogen
- Recombinant human clusterin isoform 1.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P10909
- Entrez Gene
- CLU
- GO Terms
- GO:0001836 release of cytochrome c from mitochondria
- GO:0002576 platelet degranulation
- GO:0009615 response to virus
- GO:0005739 mitochondrion
- GO:0006629 lipid metabolic process
- GO:0006958 complement activation, classical pathway
- GO:0051787 misfolded protein binding
- GO:0030168 platelet activation
- GO:0031093 platelet alpha granule lumen
- GO:0032463 negative regulation of protein homooligomerization
- GO:0034366 spherical high-density lipoprotein particle
- GO:0043691 reverse cholesterol transport
- GO:0045087 innate immune response
- GO:0051788 response to misfolded protein
- Regulatory
- For research purposes only
Applications of Clusterin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | |||
| Western Blotting | 0.5 | 1.0ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1011 detects a band of approximately 60kDa in human brain tissue lysate.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Trougakos, I.P. & Gonos, E.S. (2002) Clusterin/apolipoprotein J in human aging and cancer.
Int J Biochem Cell Biol. 34 (11): 1430-48. -
Calero, M. et al. (2005) Clusterin and Alzheimer's disease.
Subcell Biochem. 38: 273-98. -
Leskov, K.S. et al. (2003) Synthesis and functional analyses of nuclear clusterin, a cell death protein.
J Biol Chem. 278 (13): 11590-600. -
Carreras, I. et al. (2005) Upregulation of clusterin/apolipoprotein J in lactacystin-treated SH-SY5Y cells.
J Neurosci Res. 79 (4): 495-502.
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