Clusterin antibody

100% Secure


Rabbit anti Human Clusterin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1011
Datasheet
P* WB
MSDS
0.1 mg loader

Rabbit anti Human clusterin antibody recognizes human clusterin, also known as apolipoprotein J, Ku70-binding protein 1 or TRPM-2. Clusterin is a ubiquitous glycoprotein which exists in at least two distinct isoforms; a proapoptotic nuclear form, of ~49 kDa, and a prosurvival secretory form with a molecular mass of 75-80 kDa. Clusterin is implicated in several diverse physiological processes including sperm maturation, cell differentiation, and apoptosis.

Clusterin upregulation has been reported in various human malignancies, including bladder, prostate, and breast tumours.

Following specific stress induced injury, secretory clusterin acts as a molecular chaperone, scavenging denatured proteins outside cells. Clusterin upregulated in Alzheimer’s disease (Miners et al. 2017), retinitis pigmentosa (Jomary et al 1993), glomerulonephritis, atherosclerosis and myocardial infarction (Rosenberg et al. 2002).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Clusterin were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
Immunogen
Recombinant human clusterin isoform 1.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P10909
Entrez Gene
CLU
GO Terms
GO:0001836 release of cytochrome c from mitochondria
GO:0002576 platelet degranulation
GO:0009615 response to virus
GO:0005739 mitochondrion
GO:0006629 lipid metabolic process
GO:0006958 complement activation, classical pathway
GO:0051787 misfolded protein binding
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0032463 negative regulation of protein homooligomerization
GO:0034366 spherical high-density lipoprotein particle
GO:0043691 reverse cholesterol transport
GO:0045087 innate immune response
GO:0051788 response to misfolded protein
Regulatory
For research purposes only

Applications of Clusterin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
Western Blotting 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1011 detects a band of approximately 60kDa in human brain tissue lysate.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Trougakos, I.P. & Gonos, E.S. (2002) Clusterin/apolipoprotein J in human aging and cancer.
    Int J Biochem Cell Biol. 34 (11): 1430-48.
  2. Calero, M. et al. (2005) Clusterin and Alzheimer's disease.
    Subcell Biochem. 38: 273-98.
  3. Leskov, K.S. et al. (2003) Synthesis and functional analyses of nuclear clusterin, a cell death protein.
    J Biol Chem. 278 (13): 11590-600.
  4. Carreras, I. et al. (2005) Upregulation of clusterin/apolipoprotein J in lactacystin-treated SH-SY5Y cells.
    J Neurosci Res. 79 (4): 495-502.