Clusterin antibody

Rabbit anti Human Clusterin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Clusterin

Product Code Applications Pack Size List Price Your Price Qty
AHP1011
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P * WB 0.1 mg loader
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Rabbit anti Human clusterin antibody recognizes human clusterin, also known as apolipoprotein J, Ku70-binding protein 1 or TRPM-2. Clusterin is a ubiquitous glycoprotein which exists in at least two distinct isoforms; a proapoptotic nuclear form, of ~49 kDa, and a prosurvival secretory form with a molecular mass of 75-80 kDa. Clusterin is implicated in several diverse physiological processes including sperm maturation, cell differentiation, and apoptosis.

Clusterin upregulation has been reported in various human malignancies, including bladder, prostate, and breast tumours.

Following specific stress induced injury, secretory clusterin acts as a molecular chaperone, scavenging denatured proteins outside cells. Clusterin upregulated in Alzheimer’s disease (Miners et al. 2017), retinitis pigmentosa (Jomary et al 1993), glomerulonephritis, atherosclerosis and myocardial infarction (Rosenberg et al. 2002).

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Clusterin were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
Immunogen
Recombinant human clusterin isoform 1.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
Western Blotting 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1011 detects a band of approximately 60kDa in human brain tissue lysate.

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Further Reading

  1. Trougakos, I.P. & Gonos, E.S. (2002) Clusterin/apolipoprotein J in human aging and cancer.
    Int J Biochem Cell Biol. 34 (11): 1430-48.
  2. Calero, M. et al. (2005) Clusterin and Alzheimer's disease.
    Subcell Biochem. 38: 273-98.
  3. Leskov, K.S. et al. (2003) Synthesis and functional analyses of nuclear clusterin, a cell death protein.
    J Biol Chem. 278 (13): 11590-600.
  4. Carreras, I. et al. (2005) Upregulation of clusterin/apolipoprotein J in lactacystin-treated SH-SY5Y cells.
    J Neurosci Res. 79 (4): 495-502.

Synonyms
Apolipoprotein J
RRID
AB_2276520
UniProt
P10909
Entrez Gene
CLU
GO Terms
GO:0001836 release of cytochrome c from mitochondria
GO:0002576 platelet degranulation
GO:0009615 response to virus
GO:0005739 mitochondrion
GO:0006629 lipid metabolic process
GO:0006958 complement activation, classical pathway
GO:0051787 misfolded protein binding
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0032463 negative regulation of protein homooligomerization
GO:0034366 spherical high-density lipoprotein particle
GO:0043691 reverse cholesterol transport
GO:0045087 innate immune response
GO:0051788 response to misfolded protein
View more products with CLUSTERIN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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