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CDX2 antibody

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Anti Human CDX2 Antibody gallery image 1
Western blotting analysis of human kidney lysate (35μg protein in RIPA buffer) probed with Goat anti Human CDX2 antibody (AHP1568) at 1.0μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence

Goat anti Human CDX2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1568
Datasheet
E WB
MSDS
0.1 mg

Goat anti Human CDX2 antibody recognizes human CDX2 (caudal-type homeobox protein 2), a nuclear protein and member of the caudal homeobox family, which acts as a regulator for intestinal epithelium gene transcription, and possibly for the differentiation and proliferation of intestinal epithelial cells.

Expression of CDX2 in intestinal-type gastric cancer, colorectal carcinomas and intestinal metaplasia of the stomach, may serve as an early marker of Barrett's oesophagus, the premalignant lesion of oesophageal adenocarcinoma.

Goat anti Human CDX2 antibody (AHP1568) is not expected to recognise CDX1 or CDX4.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Dog Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human CDX2 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence EQLSPGGQRRNLCE corresponding to the internal region of CDX2 (NP_001256.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
Q99626 Related reagents
Entrez Gene
CDX2 Related reagents
GO Terms
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0006366 transcription from RNA polymerase II promoter
GO:0009887 organ morphogenesis
GO:0030528 transcription regulator activity
GO:0043565 sequence-specific DNA binding
Regulatory
For research purposes only

Applications of CDX2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/4000
Western Blotting 1.0 3.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1568 detects a band of approximately 38kDa in human kidney and liver cell lysates.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
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