CDKN2A antibody

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Rabbit anti CDKN2A

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1488 P* WB 0.1 mg
Rabbit anti Human CDKN2A antibody recognizes human cyclin-dependent kinase inhibitor 2A (CDKN2A), a 16 kDa protein belonging to the CDKN2 cyclin dependent kinase inhibitor family.

CDKN2A acts as a negative regulator of cell proliferation by interacting strongly with CDK4 and CDK6, thus inhibiting their ability to interact with cyclin D and to phosphorylate the retinoblastoma protein.

CDKN2A is an important tumour suppressor and defects in its production results in tumour formation in a wide range of tissues, including cutaneous malignant melanoma and Li-Fraumeni syndrome.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human CDKN2A was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A 19 amino acid peptide located near the amino terminus of human CDKN2A.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P42771 Related reagents
Q8N726 Related reagents
Entrez Gene
CDKN2A Related reagents
CDKN2A Related reagents
GO Terms
GO:0000075 cell cycle checkpoint
GO:0000080 G1 phase of mitotic cell cycle
GO:0000082 G1/S transition of mitotic cell cycle
GO:0005515 protein binding
GO:0001953 negative regulation of cell-matrix adhesion
GO:0005829 cytosol
GO:0004861 cyclin-dependent protein kinase inhibitor activity
GO:0005634 nucleus
GO:0006917 induction of apoptosis
GO:0007050 cell cycle arrest
GO:0007265 Ras protein signal transduction
GO:0008285 negative regulation of cell proliferation
GO:0019901 protein kinase binding
GO:0030308 negative regulation of cell growth
GO:0032088 negative regulation of NF-kappaB transcription factor activity
GO:0034393 positive regulation of smooth muscle cell apoptosis
GO:0045736 negative regulation of cyclin-dependent protein kinase activity
GO:0051059 NF-kappaB binding
GO:0090399 replicative senescence
GO:2000111 positive regulation of macrophage apoptosis
GO:0000209 protein polyubiquitination
GO:0002039 p53 binding
GO:0003677 DNA binding
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0006309 DNA fragmentation involved in apoptotic nuclear change
GO:0006350 transcription
GO:0006364 rRNA processing
GO:0006469 negative regulation of protein kinase activity
GO:0006919 activation of caspase activity
GO:0008637 apoptotic mitochondrial changes
GO:0010389 regulation of G2/M transition of mitotic cell cycle
GO:0016563 transcription activator activity
GO:0050821 protein stabilization
GO:0030889 negative regulation of B cell proliferation
GO:0031648 protein destabilization
GO:0033088 negative regulation of immature T cell proliferation in thymus
GO:0043234 protein complex
GO:0043517 positive regulation of DNA damage response, signal transduction by p53 class mediator
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0046825 regulation of protein export from nucleus
GO:0048103 somatic stem cell division
GO:0051444 negative regulation of ubiquitin-protein ligase activity
GO:0055105 ubiquitin-protein ligase inhibitor activity
GO:0070215 MDM2 binding
GO:0070534 protein K63-linked ubiquitination
GO:0071158 positive regulation of cell cycle arrest
GO:0090398 cellular senescence
Regulatory
For research purposes only

Applications of CDKN2A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
Western Blotting 1 2ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1488 detects a band of approximately 20 kDa in mouse colon cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Stone, S. et al. (1995) Complex structure and regulation of the P16 (MTS1) locus.
    Cancer Res. 55 (14): 2988-94.
  2. Kamb, A. et al. (1994) Analysis of the p16 gene (CDKN2) as a candidate for the chromosome 9p melanoma susceptibility locus.
    Nat Genet. 8 (1): 23-6.
  3. Kratzke, R.A. et al. (1995) Immunohistochemical analysis of the p16INK4 cyclin-dependent kinase inhibitor in malignant mesothelioma.
    J Natl Cancer Inst. 87 (24): 1870-5.
  4. Stott, F.J. et al. (1998) The alternative product from the human CDKN2A locus, p14(ARF), participates in a regulatory feedback loop with p53 and MDM2.
    EMBO J. 17 (17): 5001-14.