CD289 antibody

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Anti Human CD289 Antibody thumbnail image 2

Anti Human CD289 Antibody gallery image 1 — Western blot analysis of whole cell lysate from Jurkat human T-cell leukemia cells probed with Rabbit anti Human CD289 (**AHP1022**) at 0.5(A), 1(B) and 2(C) ug/ml

Anti Human CD289 Antibody gallery image 2 — Immunocytochemical staining of Jurkat cells with Rabbit anti Human CD289 (**AHP1022**).

Rabbit anti Human CD289

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP1022	C WB			0.1 mg

Rabbit anti Human CD289 antibody recognizes human CD289, otherwise known as toll receptor 9, a member of the evolutionarily conserved toll-like receptor family (TLRs), which play an important role in the recognition of invading microbial pathogens during innate immunity.

CD289 is expressed preferentially by the spleen, lymph node, bone marrow and peripheral blood leucocytes and recognizes viral DNA and unmethylated CpG sequences in bacterial DNA. Unlike other TLRs, TLR9 is dependant upon the adaptor molecule MyD88 for signaling activation.

Rabbit anti Human CD289 antibody has been shown as suitable for use in IHC on human Jurkat cell cytospins @ 2ug/ml.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to human CD289 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.02%

Sodium Azide (NaN₃)

Immunogen

A synthetic peptide corresponding to a 15 amino acid sequence located near the centre of human CD289.

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.

More Information

UniProt: Q9NR96 Related reagents
Entrez Gene: TLR9 Related reagents
GO Terms: GO:0002237 response to molecule of bacterial origin; GO:0042742 defense response to bacterium; GO:0005764 lysosome; GO:0016021 integral to membrane; GO:0005576 extracellular region; GO:0004888 transmembrane receptor activity; GO:0005149 interleukin-1 receptor binding; GO:0005789 endoplasmic reticulum membrane; GO:0006954 inflammatory response; GO:0007252 I-kappaB phosphorylation; GO:0008286 insulin receptor signaling pathway; GO:0008543 fibroblast growth factor receptor signaling pathway; GO:0010008 endosome membrane; GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter; GO:0016323 basolateral plasma membrane; GO:0016324 apical plasma membrane; GO:0030277 maintenance of gastrointestinal epithelium; GO:0032009 early phagosome; GO:0032088 negative regulation of NF-kappaB transcription factor activity; GO:0032715 negative regulation of interleukin-6 production; GO:0032717 negative regulation of interleukin-8 production; GO:0032722 positive regulation of chemokine production; GO:0032728 positive regulation of interferon-beta production; GO:0032733 positive regulation of interleukin-10 production; GO:0032735 positive regulation of interleukin-12 production; GO:0032741 positive regulation of interleukin-18 production; GO:0032755 positive regulation of interleukin-6 production; GO:0032757 positive regulation of interleukin-8 production; GO:0032760 positive regulation of tumor necrosis factor production; GO:0034122 negative regulation of toll-like receptor signaling pathway; GO:0034123 positive regulation of toll-like receptor signaling pathway; GO:0035197 siRNA binding; GO:0042346 positive regulation of NF-kappaB import into nucleus; GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade; GO:0043507 positive regulation of JUN kinase activity; GO:0045078 positive regulation of interferon-gamma biosynthetic process; GO:0045087 innate immune response; GO:0045356 positive regulation of interferon-alpha biosynthetic process; GO:0045359 positive regulation of interferon-beta biosynthetic process; GO:0046330 positive regulation of JNK cascade; GO:0050729 positive regulation of inflammatory response; GO:0051092 positive regulation of NF-kappaB transcription factor activity; GO:0051770 positive regulation of nitric-oxide synthase biosynthetic process
Regulatory: For research purposes only

Applications of CD289 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Frozen
Western Blotting		0.5	1.0ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP1022 detects a band of approximately 95kDa in human Jurkat cell lysates . (Predicted Mwt. 115-120kDa).

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Sheep anti Rabbit IgG:Biotin	2AB02B	1 ml	C E P WB
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	1 mg	E WB
Goat anti Rabbit IgG (Fc):FITC	STAR121F	1 mg	F
Goat anti Rabbit IgG (Fc):HRP	STAR121P	1 mg	E WB
Goat anti Rabbit IgG (H/L):HRP	STAR124P	1 mg	C E WB
Sheep anti Rabbit IgG:FITC	STAR34B	1 mg	C F
Sheep anti Rabbit IgG:RPE	STAR35A	1 ml	F
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	0.1 mg	F IF WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	0.5 ml	WB*