Rabbit anti Human CD289 antibody recognizes human CD289, otherwise known as toll receptor 9, a member of the evolutionarily conserved toll-like receptor family (TLRs), which play an important role in the recognition of invading microbial pathogens during innate immunity.
CD289 is expressed preferentially by the spleen, lymph node, bone marrow and peripheral blood leucocytes and recognizes viral DNA and unmethylated CpG sequences in bacterial DNA. Unlike other TLRs, TLR9 is dependant upon the adaptor molecule MyD88 for signaling activation.
Rabbit anti Human CD289 antibody has been shown as suitable for use in IHC on human Jurkat cell cytospins @ 2ug/ml.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human CD289 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- A synthetic peptide corresponding to a 15 amino acid sequence located near the centre of human CD289.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
response to molecule of bacterial origin
defense response to bacterium
integral to membrane
transmembrane receptor activity
interleukin-1 receptor binding
endoplasmic reticulum membrane
insulin receptor signaling pathway
fibroblast growth factor receptor signaling pathway
positive regulation of gene-specific transcription from RNA polymerase II promoter
basolateral plasma membrane
apical plasma membrane
maintenance of gastrointestinal epithelium
negative regulation of NF-kappaB transcription factor activity
negative regulation of interleukin-6 production
negative regulation of interleukin-8 production
positive regulation of chemokine production
positive regulation of interferon-beta production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-18 production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
positive regulation of tumor necrosis factor production
negative regulation of toll-like receptor signaling pathway
positive regulation of toll-like receptor signaling pathway
positive regulation of NF-kappaB import into nucleus
positive regulation of I-kappaB kinase/NF-kappaB cascade
positive regulation of JUN kinase activity
positive regulation of interferon-gamma biosynthetic process
innate immune response
positive regulation of interferon-alpha biosynthetic process
positive regulation of interferon-beta biosynthetic process
positive regulation of JNK cascade
positive regulation of inflammatory response
positive regulation of NF-kappaB transcription factor activity
positive regulation of nitric-oxide synthase biosynthetic process
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD289 antibody
|Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1022 detects a band of approximately 95kDa in human Jurkat cell lysates . (Predicted Mwt. 115-120kDa).
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Secondary Antibodies Available
Useful Reagents Available