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CD289 antibody

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Anti Human CD289 Antibody thumbnail image 1 Anti Human CD289 Antibody thumbnail image 2
Anti Human CD289 Antibody gallery image 1
Western blot analysis of whole cell lysate from Jurkat human T-cell leukemia cells probed with Rabbit anti Human CD289 (AHP1022) at 0.5(A), 1(B) and 2(C) ug/ml
Anti Human CD289 Antibody gallery image 2
Immunocytochemical staining of Jurkat cells with Rabbit anti Human CD289 (AHP1022).

Rabbit anti Human CD289

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1022
Datasheet
C WB
SDS
0.1 mg loader

Rabbit anti Human CD289 antibody recognizes human CD289, otherwise known as toll receptor 9, a member of the evolutionarily conserved toll-like receptor family (TLRs), which play an important role in the recognition of invading microbial pathogens during innate immunity.

CD289 is expressed preferentially by the spleen, lymph node, bone marrow and peripheral blood leucocytes and recognizes viral DNA and unmethylated CpG sequences in bacterial DNA. Unlike other TLRs, TLR9 is dependant upon the adaptor molecule MyD88 for signaling activation.

Rabbit anti Human CD289 antibody has been shown as suitable for use in IHC on human Jurkat cell cytospins @ 2ug/ml.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human CD289 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
Immunogen
A synthetic peptide corresponding to a 15 amino acid sequence located near the centre of human CD289.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9NR96
Entrez Gene
TLR9
GO Terms
GO:0002237 response to molecule of bacterial origin
GO:0042742 defense response to bacterium
GO:0005764 lysosome
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0004888 transmembrane receptor activity
GO:0005149 interleukin-1 receptor binding
GO:0005789 endoplasmic reticulum membrane
GO:0006954 inflammatory response
GO:0007252 I-kappaB phosphorylation
GO:0008286 insulin receptor signaling pathway
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0010008 endosome membrane
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0016323 basolateral plasma membrane
GO:0016324 apical plasma membrane
GO:0030277 maintenance of gastrointestinal epithelium
GO:0032009 early phagosome
GO:0032088 negative regulation of NF-kappaB transcription factor activity
GO:0032715 negative regulation of interleukin-6 production
GO:0032717 negative regulation of interleukin-8 production
GO:0032722 positive regulation of chemokine production
GO:0032728 positive regulation of interferon-beta production
GO:0032733 positive regulation of interleukin-10 production
GO:0032735 positive regulation of interleukin-12 production
GO:0032741 positive regulation of interleukin-18 production
GO:0032755 positive regulation of interleukin-6 production
GO:0032757 positive regulation of interleukin-8 production
GO:0032760 positive regulation of tumor necrosis factor production
GO:0034122 negative regulation of toll-like receptor signaling pathway
GO:0034123 positive regulation of toll-like receptor signaling pathway
GO:0035197 siRNA binding
GO:0042346 positive regulation of NF-kappaB import into nucleus
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043507 positive regulation of JUN kinase activity
GO:0045078 positive regulation of interferon-gamma biosynthetic process
GO:0045087 innate immune response
GO:0045356 positive regulation of interferon-alpha biosynthetic process
GO:0045359 positive regulation of interferon-beta biosynthetic process
GO:0046330 positive regulation of JNK cascade
GO:0050729 positive regulation of inflammatory response
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051770 positive regulation of nitric-oxide synthase biosynthetic process
Regulatory
For research purposes only

Applications of CD289 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Western Blotting 0.5 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1022 detects a band of approximately 95kDa in human Jurkat cell lysates . (Predicted Mwt. 115-120kDa).
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Wagner, H. (2004) The immunobiology of the TLR9 subfamily.
    Trends Immunol. 25 (7): 381-6.
  2. McGettrick, A.F. & O'Neill, L.A. (2004) The expanding family of MyD88-like adaptors in Toll-like receptor signal transduction.
    Mol Immunol. 41 (6-7): 577-82.

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