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CD286 antibody

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Anti Human CD286 Antibody thumbnail image 1 Anti Human CD286 Antibody thumbnail image 2
Anti Human CD286 Antibody gallery image 1
Western blot analysis of whole cell lysate from Jurkat human T-cell leukemia cells probed with Rabbit anti CD286 (AHP1021) at 0.5(A), 1(B) and 2(C) ug/ml
Anti Human CD286 Antibody gallery image 2
Immunocytochemical staining of Jurkat cells with Rabbit anti Human CD286 (AHP1021)

Rabbit anti Human CD286

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1021
Datasheet
C WB
SDS
0.1 mg loader

Rabbit anti Human CD286 antibody recognizes human CD286, also known as Toll-like receptor 6 (TLR6), a member of the evolutionarily conserved toll-like receptor family (TLRs) and close homologue of CD281 (TLR1), that is expressed by monocytes, CD11c+ immature dendritic cells and endothelial cells of skin microvessels.

TLRs play an important role in the recognition of invading microbial pathogens during innate immunity, acting through adaptor molecules such as MyD88 and TRAF6. CD286 acts via a cytokine signaling pathway to induce the activation of NF-kappaB, through the formation of dimers with CD282 (TLR2) which coordinate macrophage activation by Gram-positive bacteria and zymosan. CD286/CD282 dimers are also involved in apoptotic responses through recognition of mycoplasmal lipoproteins.

Rabbit anti Human CD286 antibody does not recognize CD281 (TLR1).

Rabbit anti Human CD286 antibody has been shown as suitable for use in IHC on Jurkat cell cytospins at 2ug/ml.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human CD286 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A synthetic peptide corresponding to a 13 amino acid sequence located near the centre of human CD286.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9Y2C9
Entrez Gene
TLR6
GO Terms
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0042742 defense response to bacterium
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0006954 inflammatory response
GO:0007250 activation of NF-kappaB-inducing kinase activity
GO:0008063 Toll signaling pathway
GO:0030670 phagocytic vesicle membrane
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0042088 T-helper 1 type immune response
GO:0042496 detection of diacyl bacterial lipopeptide
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043507 positive regulation of JUN kinase activity
GO:0045087 innate immune response
GO:0045410 positive regulation of interleukin-6 biosynthetic process
GO:0071726 cellular response to diacyl bacterial lipopeptide
Regulatory
For research purposes only

Applications of CD286 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Western Blotting 0.5 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1021 detects a band of approximately 98kDa and 110kDa in human Jurkat cell lysates (predicted Mwt. 91.9kDa). Doublet band may be due to protein modification or the recognition of two different isoforms by the same gene.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Into, T. et al. (2004) Stimulation of human Toll-like receptor (TLR) 2 and TLR6 with membrane lipoproteins of Mycoplasma fermentans induces apoptotic cell death after NF-kappa B activation.
    Cell Microbiol. 6 (2): 187-99.
  2. Ozinsky, A. et al. (2000) The repertoire for pattern recognition of pathogens by the innate immune system is defined by cooperation between toll-like receptors.
    Proc Natl Acad Sci U S A. 97 (25): 13766-71.

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Request a different product with the CD286 specificity