CD286 antibody
Rabbit anti Human CD286 antibody recognizes human CD286, also known as Toll-like receptor 6 (TLR6), a member of the evolutionarily conserved toll-like receptor family (TLRs) and close homologue of CD281 (TLR1), that is expressed by monocytes, CD11c+ immature dendritic cells and endothelial cells of skin microvessels.
TLRs play an important role in the recognition of invading microbial pathogens during innate immunity, acting through adaptor molecules such as MyD88 and TRAF6. CD286 acts via a cytokine signaling pathway to induce the activation of NF-kappaB, through the formation of dimers with CD282 (TLR2) which coordinate macrophage activation by Gram-positive bacteria and zymosan. CD286/CD282 dimers are also involved in apoptotic responses through recognition of mycoplasmal lipoproteins.
Rabbit anti Human CD286 antibody does not recognize CD281 (TLR1).
Rabbit anti Human CD286 antibody has been shown as suitable for use in IHC on Jurkat cell cytospins at 2ug/ml.
TLRs play an important role in the recognition of invading microbial pathogens during innate immunity, acting through adaptor molecules such as MyD88 and TRAF6. CD286 acts via a cytokine signaling pathway to induce the activation of NF-kappaB, through the formation of dimers with CD282 (TLR2) which coordinate macrophage activation by Gram-positive bacteria and zymosan. CD286/CD282 dimers are also involved in apoptotic responses through recognition of mycoplasmal lipoproteins.
Rabbit anti Human CD286 antibody does not recognize CD281 (TLR1).
Rabbit anti Human CD286 antibody has been shown as suitable for use in IHC on Jurkat cell cytospins at 2ug/ml.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human CD286 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- A synthetic peptide corresponding to a 13 amino acid sequence located near the centre of human CD286.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9Y2C9
- Entrez Gene
- TLR6
- GO Terms
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0042742 defense response to bacterium
- GO:0004888 transmembrane receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006954 inflammatory response
- GO:0007250 activation of NF-kappaB-inducing kinase activity
- GO:0008063 Toll signaling pathway
- GO:0030670 phagocytic vesicle membrane
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0042088 T-helper 1 type immune response
- GO:0042496 detection of diacyl bacterial lipopeptide
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0043507 positive regulation of JUN kinase activity
- GO:0045087 innate immune response
- GO:0045410 positive regulation of interleukin-6 biosynthetic process
- GO:0071726 cellular response to diacyl bacterial lipopeptide
- Regulatory
- For research purposes only
Applications of CD286 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | |||
| Western Blotting | 0.5 | 1.0ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1021 detects a band of approximately 98kDa and 110kDa in human Jurkat cell lysates (predicted Mwt. 91.9kDa). Doublet band may be due to protein modification or the recognition of two different isoforms by the same gene.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Into, T. et al. (2004) Stimulation of human Toll-like receptor (TLR) 2 and TLR6 with membrane lipoproteins of Mycoplasma fermentans induces apoptotic cell death after NF-kappa B activation.
Cell Microbiol. 6 (2): 187-99. -
Ozinsky, A. et al. (2000) The repertoire for pattern recognition of pathogens by the innate immune system is defined by cooperation between toll-like receptors.
Proc Natl Acad Sci U S A. 97 (25): 13766-71.
