Rabbit anti Human CD281 antibody detects an epitope within the N-terminal (NT) region of CD281, also known as toll like receptor 1 (TLR1). TLRs recognize pathogen associated molecular patterns (PAMPs) that are expressed on infectious agents, and serve as an important link between the innate and adaptive immune response through the production of cytokines. TLR1 is co-expressed with TLR2 on monocytes and dendritic cells where they form heterodimers that can recognize peptidoglycan and bacterial lipopeptides, and in particular triacylated lipoproteins.
TLR1 can also regulate other TLR responses; TLR1 can associate with TLR4 and inhibit its signalling in endothelial cells, and can associate with TLR2 to inhibit the TLR2/TLR6 mediated response to phenol-soluble modulin.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to CD281 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- A 16 amino acid peptide located near the amino terminus of human CD281.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
transmembrane receptor activity
integral to plasma membrane
phagocytic vesicle membrane
Toll-like receptor 1-Toll-like receptor 2 protein complex
detection of triacyl bacterial lipopeptide
positive regulation of tumor necrosis factor biosynthetic process
innate immune response
positive regulation of interleukin-6 biosynthetic process
protein heterodimerization activity
cellular response to triacyl bacterial lipopeptide
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD281 antibody
|Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Mouse spleen.
- Western Blotting
- Rabbit anti Human CD281 detects a band of approximately 90kDa in mouse spleen lysates.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Hajjar, A.M .et al. (2001) Cutting edge: functional interactions between toll-like receptor (TLR) 2 and TLR1 or TLR6 in response to phenol-soluble modulin.
J Immunol. 166 (1): 15-9.
Janssens, S. & Beyaert, R. (2003) Role of Toll-like receptors in pathogen recognition.
Clin Microbiol Rev. 16 (4): 637-46.
Spitzer JH et al. (2002) Toll-like receptor 1 inhibits Toll-like receptor 4 signaling in endothelial cells.
Eur J Immunol. 32 (4): 1182-7.
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