CD281 antibody

100% Secure


Rabbit anti Human CD281 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1181 C WB 0.1 mg
Rabbit anti Human CD281 antibody detects an epitope within the N-terminal (NT) region of CD281, also known as toll like receptor 1 (TLR1). TLRs recognize pathogen associated molecular patterns (PAMPs) that are expressed on infectious agents, and serve as an important link between the innate and adaptive immune response through the production of cytokines. TLR1 is co-expressed with TLR2 on monocytes and dendritic cells where they form heterodimers that can recognize peptidoglycan and bacterial lipopeptides, and in particular triacylated lipoproteins.

TLR1 can also regulate other TLR responses; TLR1 can associate with TLR4 and inhibit its signalling in endothelial cells, and can associate with TLR2 to inhibit the TLR2/TLR6 mediated response to phenol-soluble modulin.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to CD281 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
Immunogen
A 16 amino acid peptide located near the amino terminus of human CD281.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q15399 Related reagents
Entrez Gene
TLR1 Related reagents
GO Terms
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0006954 inflammatory response
GO:0030670 phagocytic vesicle membrane
GO:0035354 Toll-like receptor 1-Toll-like receptor 2 protein complex
GO:0042116 macrophage activation
GO:0042495 detection of triacyl bacterial lipopeptide
GO:0042535 positive regulation of tumor necrosis factor biosynthetic process
GO:0045087 innate immune response
GO:0045410 positive regulation of interleukin-6 biosynthetic process
GO:0046982 protein heterodimerization activity
GO:0071727 cellular response to triacyl bacterial lipopeptide
Regulatory
For research purposes only

Applications of CD281 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 10ug/ml
Western Blotting 1 4ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Mouse spleen.
Western Blotting
Rabbit anti Human CD281 detects a band of approximately 90kDa in mouse spleen lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Hajjar, A.M .et al. (2001) Cutting edge: functional interactions between toll-like receptor (TLR) 2 and TLR1 or TLR6 in response to phenol-soluble modulin.
    J Immunol. 166 (1): 15-9.
  2. Janssens, S. & Beyaert, R. (2003) Role of Toll-like receptors in pathogen recognition.
    Clin Microbiol Rev. 16 (4): 637-46.
  3. Spitzer JH et al. (2002) Toll-like receptor 1 inhibits Toll-like receptor 4 signaling in endothelial cells.
    Eur J Immunol. 32 (4): 1182-7.