CD281 antibody
Rabbit anti Human CD281 antibody detects an epitope within the N-terminal (NT) region of CD281, also known as toll like receptor 1 (TLR1). TLRs recognize pathogen associated molecular patterns (PAMPs) that are expressed on infectious agents, and serve as an important link between the innate and adaptive immune response through the production of cytokines. TLR1 is co-expressed with TLR2 on monocytes and dendritic cells where they form heterodimers that can recognize peptidoglycan and bacterial lipopeptides, and in particular triacylated lipoproteins.
TLR1 can also regulate other TLR responses; TLR1 can associate with TLR4 and inhibit its signalling in endothelial cells, and can associate with TLR2 to inhibit the TLR2/TLR6 mediated response to phenol-soluble modulin.
TLR1 can also regulate other TLR responses; TLR1 can associate with TLR4 and inhibit its signalling in endothelial cells, and can associate with TLR2 to inhibit the TLR2/TLR6 mediated response to phenol-soluble modulin.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to CD281 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide (NaN3) - Immunogen
- A 16 amino acid peptide located near the amino terminus of human CD281.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q15399
- Entrez Gene
- TLR1
- GO Terms
- GO:0004888 transmembrane receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006954 inflammatory response
- GO:0030670 phagocytic vesicle membrane
- GO:0035354 Toll-like receptor 1-Toll-like receptor 2 protein complex
- GO:0042116 macrophage activation
- GO:0042495 detection of triacyl bacterial lipopeptide
- GO:0042535 positive regulation of tumor necrosis factor biosynthetic process
- GO:0045087 innate immune response
- GO:0045410 positive regulation of interleukin-6 biosynthetic process
- GO:0046982 protein heterodimerization activity
- GO:0071727 cellular response to triacyl bacterial lipopeptide
- Regulatory
- For research purposes only
Applications of CD281 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | 10ug/ml | ||
| Western Blotting | 1 | 4ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Mouse spleen.
- Western Blotting
- Rabbit anti Human CD281 detects a band of approximately 90kDa in mouse spleen lysates.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
|
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Hajjar, A.M .et al. (2001) Cutting edge: functional interactions between toll-like receptor (TLR) 2 and TLR1 or TLR6 in response to phenol-soluble modulin.
J Immunol. 166 (1): 15-9. -
Janssens, S. & Beyaert, R. (2003) Role of Toll-like receptors in pathogen recognition.
Clin Microbiol Rev. 16 (4): 637-46. -
Spitzer JH et al. (2002) Toll-like receptor 1 inhibits Toll-like receptor 4 signaling in endothelial cells.
Eur J Immunol. 32 (4): 1182-7.
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