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CD261 antibody

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Anti Human CD261 (N-Terminal) Antibody gallery image 1
Western blot analysis for whole cell lysates from HeLa (H), K562 (K) and Jurkat (J) cell lines probed with Rabbit anti Human CD261 (AHP438).

Rabbit anti Human CD261 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP438
Datasheet
WB
SDS
0.1 mg loader

Rabbit anti Human CD261 antibody recognizes the ~57 kDa apoptosis related protein designated Death receptor 4, also known as CD261 or DR4. CD261 is a cell surface protein with a death domain that has functional similarities to TNFR1 and CD95. The ligand for DR-4 is TRAIL, and thus CD261 has also been called TRAIL-RI. DR4 is expressed in most human tissues. In contrast to CD95, TNFR1 and DR3 it does not use FADD to transmit death signals.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to CD261 (DR4) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
Peptide from N-terminus of human CD261 (DR4)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
O00220
Entrez Gene
TNFRSF10A
GO Terms
GO:0016021 integral to membrane
GO:0005035 death receptor activity
GO:0006919 activation of caspase activity
GO:0007250 activation of NF-kappaB-inducing kinase activity
GO:0008134 transcription factor binding
GO:0008625 induction of apoptosis via death domain receptors
GO:0008656 caspase activator activity
GO:0045569 TRAIL binding
Regulatory
For research purposes only

Applications of CD261 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Pan, G. et al. (1997) The receptor for the cytotoxic ligand TRAIL.
    Science. 276 (5309): 111-3.

Apoptosis Resources

Apoptosis Phases

Apoptosis Webinar

Apoptosis Kits

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