CD256 antibody

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Anti Human CD256 Antibody thumbnail image 2

Anti Human CD256 Antibody gallery image 1 — Western blot analysis of whole cell lysates from Jurkat cells probed with Rabbit anti Human CD256 (**AHP953**) also known as April at 1(A), 2(B) and 4(C) ug/ml.

Anti Human CD256 Antibody gallery image 2 — Immunohistochemistry image of human prostate section stained with Rabbit anti Human CD256 antibody (**AHP953**).

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WB P

Rabbit anti Human CD256

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: CD256

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AHP953 Datasheet Datasheet SDS SDS	P WB	0.1 mg		Log in	Get Quote
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Rabbit anti Human CD256 antibody recognizes Tumor necrosis factor ligand superfamily member 13, also known as CD256, A proliferation-inducing ligand, APRIL, TNF- and APOL-related leukocyte expressed ligand 2 or TNF-related death ligand 1.

CD256 is a member of the tumour necrosis factor family, expressed in B cells and several tumour cells. The protein is involved in stimulating B and T cell proliferation, triggering humoral immune responses, activating NF-kappaB and inducing cell death. CD256 is thought to play a role in autoimmune diseases and cancer.

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Rat

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Antiserum Preparation: Antisera to human CD256 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.02% Sodium Azide
Immunogen: Synthetic peptide corresponding to amino acids 196 to 210 of human CD256.
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Rat
Mouse

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin			1/100
Western Blotting		1/250	1/1000

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP953 detects a band approximately 27kD in Jurkat cell lysates

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Synonyms: April
RRID: AB_567262
UniProt: O75888
Entrez Gene: TNFSF13
GO Terms: GO:0016020 membrane; GO:0007165 signal transduction; GO:0005125 cytokine activity; GO:0005164 tumor necrosis factor receptor binding; GO:0005615 extracellular space; GO:0005654 nucleoplasm; GO:0006955 immune response; GO:0008284 positive regulation of cell proliferation; GO:0016071 mRNA metabolic process; View More GO Terms; GO:0048298 positive regulation of isotype switching to IgA isotypes