CD193 antibody

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Rabbit anti Human CD193

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1010 C IF P* WB 0.1 mg
Rabbit anti Human CD193 antibody recognizes human CD193, otherwise known as CC chemokine receptor 3 (CCR3), a seven-helix transmembrane G-protein coupled receptor which, along with CCR5 and CXCR4, acts as a principal receptor for the fusion and entry of HIV into target cells. CD193 is expressed by eosinophils, basophils, mast cells, microglia, Th2 helper cells and some epithelial cells.

CD193 acts as a receptor for several CC chemokines including monocyte chemoattractant proteins (MCP) -1, -2, -3 and -4, MIP-1a, MIP-1β, and RANTES. The eotaxin subgroup of CC chemokines act as specific agonists for CD193, and this interaction has become a focus area for studies into the infection of microglia by HIV (He, J. et al. 1997) and allergy research (Holse, M. et al. 2006).

Rabbit anti Human CD193 antibody has been used successfully for the detection of human CD193 by immunohistochemistry on formalin fixed, paraffin embedded intervertebral disc tissue (Gruber et al. 2014).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human CD193 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to a 14 amino acid sequence located near human CD193 carboxy-terminus.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P51677 Related reagents
Entrez Gene
CCR3 Related reagents
GO Terms
GO:0007155 cell adhesion
GO:0005887 integral to plasma membrane
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0006968 cellular defense response
GO:0007188 G-protein signaling, coupled to cAMP nucleotide second messenger
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0044419 interspecies interaction between organisms
GO:0045766 positive regulation of angiogenesis
Regulatory
For research purposes only

Applications of CD193 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1 10ug/ml
Western Blotting 1ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human spleen.
Western Blotting
AHP1010 detects a band of approximately 55kDa in human spleen cell lysates (predicted Mwt. 41kDa)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Application Based External Images

Immunofluorescence

Immunohistology - Frozen

Product Specific References

References for CD193 antibody

  1. Wainwright, D.A. et al. (2009) Exacerbation of facial motoneuron loss after facial nerve axotomy in CCR3-deficient mice.
    ASN Neuro. 1 (5): e00024.
  2. Kallankari, H. et al. (2010) Chemokine CCL18 predicts intraventricular hemorrhage in very preterm infants.
    Ann Med. 42: 416-25.
  3. Gruber, H.E. et al. (2014) Production and expression of RANTES (CCL5) by human disc cells and modulation by IL-1-β and TNF-α in 3D culture.
    Exp Mol Pathol. 96: 133-8.

Further Reading

  1. Holse, M. et al. (2006) CCR3, CCR5, CCR8 and CXCR3 expression in memory T helper cells from allergic rhinitis patients, asymptomatically sensitized and healthy individuals.
    Clin Mol Allergy. 4: 6.
  2. Combadiere, C. et al. (1996) Cloning and functional expression of a human eosinophil CC chemokine receptor.
    J Biol Chem. 271 (18): 11034.
  3. He, J. et al. (1997) CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia.
    Nature. 385 (6617): 645-9.